CAJ | 학술논문

以甘肃红豆草为材料，采用 RT－PCR 法克隆无色花青素还原酶 LAR 基因，分析该功能基因在甘肃红豆草不同器官表达的差异性。结果表明，克隆的甘肃红豆草 LAR 基因，与 GenBank 已报道 LAR 基因（登录号：HM152981）序列相似性达到98．34％，其 ORF 长为1089 bp，编码362个氨基酸残基；其编码的氨基酸序列与不同属豆科物种的相似性存在较大的差异。基因序列已注册到 GenBank，序列登录号为 KP013623。LAR 基因在甘肃红豆草不同器官表达差异较大；其中叶中表达量最高，其次是花和果，茎中表达量最低，这与器官间缩合单宁含量的变化规律一致。推测其表达与甘肃红豆草缩合单宁器官间的积累差异有关。这些研究结果也为探索缩合单宁积累和表达调控的机制提供基础。
이감숙홍두초위재료，채용 RT－PCR 법극륭무색화청소환원매 LAR 기인，분석해공능기인재감숙홍두초불동기관표체적차이성。결과표명，극륭적감숙홍두초 LAR 기인，여 GenBank 이보도 LAR 기인（등록호：HM152981）서렬상사성체도98．34％，기 ORF 장위1089 bp，편마362개안기산잔기；기편마적안기산서렬여불동속두과물충적상사성존재교대적차이。기인서렬이주책도 GenBank，서렬등록호위 KP013623。LAR 기인재감숙홍두초불동기관표체차이교대；기중협중표체량최고，기차시화화과，경중표체량최저，저여기관간축합단저함량적변화규률일치。추측기표체여감숙홍두초축합단저기관간적적루차이유관。저사연구결과야위탐색축합단저적루화표체조공적궤제제공기출。
A leucoanthocyanidin reductase (LAR)gene was cloned using RT-PCR from Onobrychis viciifolia (sainfoin)cv.Gansu,and the level of LAR gene expression was analyzed in different plant organs.The LAR gene sequence from O.viciifolia cv.Gansu shared a high level of similarity (98.34% homology)with an O. viciifolia LAR gene in GenBank (accession No:HM152980).However,the coding sequence of amino acids is obviously different from those in other genera of the Fabaceae.The open reading frame (ORF)comprised 1089 bp,encoding 362 amino acid residues.The sequence of the LAR gene from the present study was submitted to GenBank with the accession number KP013623.LAR expression differed between plant organs of O.viciifolia cv.Gansu.LAR expression was highest in leaves,followed by flowers and fruit,and was lowest in stem tis-sues,which corresponded to concentration of condensed tannins in different organs of O.viciifolia cv.Gansu. It is inferred that LAR gene expression is in some way involved in condensed tannin accumulation in O.viciifoliacv.Gansu.These results provide fundamental information and a method for exploring the regulation mecha-nism of condensed tannin accumulation.