草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2015年
6期
150-158
,共9页
黄玉梅%张杨雪%刘庆林%黄胜岚%刘盼
黃玉梅%張楊雪%劉慶林%黃勝嵐%劉盼
황옥매%장양설%류경림%황성람%류반
孔雀草%园林植物%水浸提液%化感作用
孔雀草%園林植物%水浸提液%化感作用
공작초%완림식물%수침제액%화감작용
Tagetes patula%garden plants%aqueous extracts%allelopathy
以4种园林植物石竹、千叶蓍、二月兰、鸡冠花作为受体,采用室内培养皿法,研究了不同浓度孔雀草水浸提液(0,12.5,25.0,50.0,100.0 mg/mL)对4种园林植物种子萌发及幼苗生长的化感作用,旨在为孔雀草在园林植物配置中的应用提供科学依据。结果表明,孔雀草水浸提液对4种园林植物种子萌发及生长有明显影响,且4种园林植物间存在一定差异。孔雀草水浸提液对石竹、二月兰、鸡冠花种子萌发均表现为抑制作用,对千叶蓍种子则表现为一定的促进作用;孔雀草水浸提液对石竹幼苗表现为抑制作用,而对二月兰和鸡冠花幼苗表现为“低促高抑”,浓度为100.0 mg/mL 时,抑制作用最强;千叶蓍幼苗生长在浸提液浓度为50.0 mg/mL 时仍表现为显著的促进作用,仅在100.0 mg/mL 时受到一定程度抑制;孔雀草水浸提液对石竹、鸡冠花和二月兰幼苗叶绿素含量基本都表现为抑制作用,千叶蓍仅在100.0 mg/mL 时表现为抑制;二月兰、鸡冠花幼苗 POD 活性在中低浓度时有不同程度升高,100.0 mg/mL 时呈下降趋势,石竹幼苗 POD 活性随浸提液浓度升高持续下降,千叶蓍则先下降后升高;石竹和鸡冠花幼苗 MDA 含量随浸提液浓度升高而升高,千叶蓍和二月兰则先下降后升高。
以4種園林植物石竹、韆葉蓍、二月蘭、鷄冠花作為受體,採用室內培養皿法,研究瞭不同濃度孔雀草水浸提液(0,12.5,25.0,50.0,100.0 mg/mL)對4種園林植物種子萌髮及幼苗生長的化感作用,旨在為孔雀草在園林植物配置中的應用提供科學依據。結果錶明,孔雀草水浸提液對4種園林植物種子萌髮及生長有明顯影響,且4種園林植物間存在一定差異。孔雀草水浸提液對石竹、二月蘭、鷄冠花種子萌髮均錶現為抑製作用,對韆葉蓍種子則錶現為一定的促進作用;孔雀草水浸提液對石竹幼苗錶現為抑製作用,而對二月蘭和鷄冠花幼苗錶現為“低促高抑”,濃度為100.0 mg/mL 時,抑製作用最彊;韆葉蓍幼苗生長在浸提液濃度為50.0 mg/mL 時仍錶現為顯著的促進作用,僅在100.0 mg/mL 時受到一定程度抑製;孔雀草水浸提液對石竹、鷄冠花和二月蘭幼苗葉綠素含量基本都錶現為抑製作用,韆葉蓍僅在100.0 mg/mL 時錶現為抑製;二月蘭、鷄冠花幼苗 POD 活性在中低濃度時有不同程度升高,100.0 mg/mL 時呈下降趨勢,石竹幼苗 POD 活性隨浸提液濃度升高持續下降,韆葉蓍則先下降後升高;石竹和鷄冠花幼苗 MDA 含量隨浸提液濃度升高而升高,韆葉蓍和二月蘭則先下降後升高。
이4충완림식물석죽、천협시、이월란、계관화작위수체,채용실내배양명법,연구료불동농도공작초수침제액(0,12.5,25.0,50.0,100.0 mg/mL)대4충완림식물충자맹발급유묘생장적화감작용,지재위공작초재완림식물배치중적응용제공과학의거。결과표명,공작초수침제액대4충완림식물충자맹발급생장유명현영향,차4충완림식물간존재일정차이。공작초수침제액대석죽、이월란、계관화충자맹발균표현위억제작용,대천협시충자칙표현위일정적촉진작용;공작초수침제액대석죽유묘표현위억제작용,이대이월란화계관화유묘표현위“저촉고억”,농도위100.0 mg/mL 시,억제작용최강;천협시유묘생장재침제액농도위50.0 mg/mL 시잉표현위현저적촉진작용,부재100.0 mg/mL 시수도일정정도억제;공작초수침제액대석죽、계관화화이월란유묘협록소함량기본도표현위억제작용,천협시부재100.0 mg/mL 시표현위억제;이월란、계관화유묘 POD 활성재중저농도시유불동정도승고,100.0 mg/mL 시정하강추세,석죽유묘 POD 활성수침제액농도승고지속하강,천협시칙선하강후승고;석죽화계관화유묘 MDA 함량수침제액농도승고이승고,천협시화이월란칙선하강후승고。
In order to provide scientific theoretical basis for the application of Tagetes patula in the garden plants configuration,the aqueous extracts from Tagetes patula was used to search the allelopathic effects on seed germination and seedling growth of Dianthus chinensis ,Achillea millefolium ,Orychophragmus viola-ceus ,Celosia cristata using petridishs with filter papers.Results showed that the aqueous extracts from Tagetes patula had significant allelopathic effects on seed germination and seedling growth of four garden plants.The aqueous extracts from Tagetes patula inhibited seed germination of D .chinensis ,O.violaceus and C.cristata,on the contrary,inceased seed germination of A.millefolium in a degree.The extracts also inhibi-ted seedling growth of D .chinensis at all concentrations,but promoted at the lower concentrations and inhibi-ted at the higher ones the seedling growth of O.violaceus and C.cristata.The inhibition was the most inten-sive by treatment with 100.0 mg/mL of the aqueous extracts.Whereas,the growth of A.millefolium was pro-moted significantly at 12.5,25.0,50.0 mg/mL,and inhibited only by treatment with 100.0 mg/mL.The chlorophyll content in these four garden plants were reduced by 23.46%,42.56%,14.75% and 58.28%, when the aqueous extracts concentration was 100.0 mg/mL.The extracts increased the activities of peroxidase(POD)of O.violaceus and C.cristata at the lower concentration,while decreased the activities of POD at 100.0 mg/mL.The activity of POD of D .chinensis declined at all concentrations,while A.millefolium de-clined earlier then ascended later.The content of MDA of D .chinensis and C.cristata ascended at all concen-trations,whereas A.millefolium and O.violaceus declined earlier then ascended later.It implied that T.pat-ula had different allelopathic effects on different garden plants and “concentration effect”was found in this research.