化学研究与应用
化學研究與應用
화학연구여응용
CHEMICAL RESEARCH AND APPLICATION
2015年
6期
882-885
,共4页
卢定强%孙生柏%凌岫泉%王琦%夏芙洁
盧定彊%孫生柏%凌岫泉%王琦%夏芙潔
로정강%손생백%릉수천%왕기%하부길
西那卡塞%对映体拆分%Chiralpak AD-H%高效液相色谱
西那卡塞%對映體拆分%Chiralpak AD-H%高效液相色譜
서나잡새%대영체탁분%Chiralpak AD-H%고효액상색보
cinacalcet%enantiomeric separation%Chiralpak AD-H%HPLC
建立了一种快速、准确的高效液相色谱分析方法。使用Chiralpak AD-H手性色谱柱,检测波长224nm,考察了流动相中极性调节剂的体积分数、流动相中三乙胺的体积分数、柱温及流速对西那卡塞对映体拆分的影响。确立了最佳拆分条件:流动相为正己烷-异丙醇-三乙胺(90∶10∶0.1,V/V/V);流速为1.0 mL·min-1;柱温为30℃。在此优化实验条件下,得到西那卡塞对映体的出峰顺序为:先S体,后R体。所建立的方法简单准确,重复性好,可用于西那卡塞的质量研究和控制。
建立瞭一種快速、準確的高效液相色譜分析方法。使用Chiralpak AD-H手性色譜柱,檢測波長224nm,攷察瞭流動相中極性調節劑的體積分數、流動相中三乙胺的體積分數、柱溫及流速對西那卡塞對映體拆分的影響。確立瞭最佳拆分條件:流動相為正己烷-異丙醇-三乙胺(90∶10∶0.1,V/V/V);流速為1.0 mL·min-1;柱溫為30℃。在此優化實驗條件下,得到西那卡塞對映體的齣峰順序為:先S體,後R體。所建立的方法簡單準確,重複性好,可用于西那卡塞的質量研究和控製。
건립료일충쾌속、준학적고효액상색보분석방법。사용Chiralpak AD-H수성색보주,검측파장224nm,고찰료류동상중겁성조절제적체적분수、류동상중삼을알적체적분수、주온급류속대서나잡새대영체탁분적영향。학립료최가탁분조건:류동상위정기완-이병순-삼을알(90∶10∶0.1,V/V/V);류속위1.0 mL·min-1;주온위30℃。재차우화실험조건하,득도서나잡새대영체적출봉순서위:선S체,후R체。소건립적방법간단준학,중복성호,가용우서나잡새적질량연구화공제。
A quantitative analysis method for the determination of cinacalcet enantiomers by HPLC had been developed. The enanti-omers of cinacalcet were separated on a Chiralpak AD-H column and the UV detection wavelength was 224 nm. The influences of concentration of organic solvent,proportion of triethylamine,column temperature and flow rate on the enantiomeric separation were investigated. The optimal chromatographic conditions were as follows:the mobile phase was hexane-isopropanol-triethylamine(90∶10∶0. 1,V/V/V);the flow rate was 1. 0 mL·min-1;the column temperature was 30℃. Under the optimal conditions,the elution order of two enantiomers was S-cinacalcet before R-cinacalcet. It was simple,accurate,and reproducible HPLC method,and could be used for the analysis and quality control of cinacalcet.