现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2015年
13期
1879-1882
,共4页
骞佩%崔兆勋%王亚芳%庞海林%聂娜%张贺龙%刘理礼
鶱珮%崔兆勛%王亞芳%龐海林%聶娜%張賀龍%劉理禮
건패%최조훈%왕아방%방해림%섭나%장하룡%류리례
Sema3A%胃癌%临床意义
Sema3A%胃癌%臨床意義
Sema3A%위암%림상의의
Sema3A%gastric cancer%clinical significance
目的：探讨Sema3A在胃癌中的表达及意义。方法：利用酶联免疫吸附法（ ELISA）检测正常健康人及胃癌患者血清中Sema3A表达的差异性。应用实时荧光定量聚合酶链反应（ Real time PCR）检测胃癌细胞系、人正常胃黏膜细胞中Sema3A mRNA的表达差异。应用免疫印迹法（ Western Blot）检测胃癌细胞系、人正常胃黏膜细胞中Sema3A 蛋白的表达差异。结果：与正常健康对照者相比，Sema3A在胃癌患者血清中表达下降（P＜0.001），且与胃癌患者的年龄、肿瘤分化程度相关（P＜0.05）。人转移潜能胃癌细胞株MKN-28M、MKN-28NM中Sema3A mRNA和蛋白表达量明显低于人正常胃黏膜细胞（ P＜0.05），且在高转移潜能MKN-28M细胞中表达量最低（ P＜0.05）。结论：与正常对照组相比，Sema3A在胃癌患者血清、人胃癌细胞株中均表达下降，且在胃癌高转移潜能MKN-28M细胞中表达最低，提示Sema3A在胃癌的发生发展中具有重要作用，可能扮演着抑癌基因的作用。
目的：探討Sema3A在胃癌中的錶達及意義。方法：利用酶聯免疫吸附法（ ELISA）檢測正常健康人及胃癌患者血清中Sema3A錶達的差異性。應用實時熒光定量聚閤酶鏈反應（ Real time PCR）檢測胃癌細胞繫、人正常胃黏膜細胞中Sema3A mRNA的錶達差異。應用免疫印跡法（ Western Blot）檢測胃癌細胞繫、人正常胃黏膜細胞中Sema3A 蛋白的錶達差異。結果：與正常健康對照者相比，Sema3A在胃癌患者血清中錶達下降（P＜0.001），且與胃癌患者的年齡、腫瘤分化程度相關（P＜0.05）。人轉移潛能胃癌細胞株MKN-28M、MKN-28NM中Sema3A mRNA和蛋白錶達量明顯低于人正常胃黏膜細胞（ P＜0.05），且在高轉移潛能MKN-28M細胞中錶達量最低（ P＜0.05）。結論：與正常對照組相比，Sema3A在胃癌患者血清、人胃癌細胞株中均錶達下降，且在胃癌高轉移潛能MKN-28M細胞中錶達最低，提示Sema3A在胃癌的髮生髮展中具有重要作用，可能扮縯著抑癌基因的作用。
목적：탐토Sema3A재위암중적표체급의의。방법：이용매련면역흡부법（ ELISA）검측정상건강인급위암환자혈청중Sema3A표체적차이성。응용실시형광정량취합매련반응（ Real time PCR）검측위암세포계、인정상위점막세포중Sema3A mRNA적표체차이。응용면역인적법（ Western Blot）검측위암세포계、인정상위점막세포중Sema3A 단백적표체차이。결과：여정상건강대조자상비，Sema3A재위암환자혈청중표체하강（P＜0.001），차여위암환자적년령、종류분화정도상관（P＜0.05）。인전이잠능위암세포주MKN-28M、MKN-28NM중Sema3A mRNA화단백표체량명현저우인정상위점막세포（ P＜0.05），차재고전이잠능MKN-28M세포중표체량최저（ P＜0.05）。결론：여정상대조조상비，Sema3A재위암환자혈청、인위암세포주중균표체하강，차재위암고전이잠능MKN-28M세포중표체최저，제시Sema3A재위암적발생발전중구유중요작용，가능분연착억암기인적작용。
Objective:To investigate the exPression of SemaPhorin 3A in gastric carcinoma and its clinical signifi-cance. Methods:The concentration of Sema3A in the serum was detected by the enzyme-linked immune sorbent as-say(ELISA)in 104 Patients with gastric cancer,20 healthy controls. Sema3A was detected in high and low metastatic gastric cancer cell lines through RT-qPCR and Western Blot method. Results:Sema3A exPression in gastric cancer Patients serum decreased(P<0. 001)and was associated with stomach cancer Patients age and tumor differentiation degree(P<0. 05). Gastric cancer transferred cell lines MKN-28M,MKN-28NM in Sema3A mRNA and Protein exPression was significantly less than the normal human umbilical vein endothelial cells(P<0. 01),but not in SGC-7901 cell lines(P>0. 05). In addition,the exPressing of Sema3A was quantity minimum in MKN-28M cells. Con-clusion:The exPression of Sema3A declined in gastric cancer Patients serum and gastric cancer cell lines,which are metastatic Potential. In addition,the exPression of Sema3A was quantity minimum in high metastasis Potential gastric cancer MKN-28M cells. Sema3A,as a tumor suPPressor,Plays an imPortant role in inhibiting gastric cancer develoP-ment.
