西南军医
西南軍醫
서남군의
JOURNAL OF MILITARY SURGEON IN SOUTHWEST CHINA
2015年
3期
269-273
,共5页
角膜碱烧伤%色素上皮衍生因子%纳米粒%药物缓释%角膜新生血管
角膜堿燒傷%色素上皮衍生因子%納米粒%藥物緩釋%角膜新生血管
각막감소상%색소상피연생인자%납미립%약물완석%각막신생혈관
cornea alkali burn%pigment epithelium-derived factor (PEDF)%nanoparticle%drug release%neovascularization
目的：应用离子交联法制备色素上皮衍生因子壳聚糖（PEDF/CS）纳米粒,考察其体外性质及其在兔角膜碱烧伤中对角膜新生血管的影响，探讨其作用机制。方法通过离子交联法制备PEDF/CS纳米粒，考察纳米粒粒径、包封率、zeta电位及体外释放量，建立兔角膜碱烧伤模型，右眼作为实验眼。单纯随机抽样法，分为生理盐水对照组(A组)、PEDF滴眼液组(B组)、PEDF/CS纳米粒组(C组)，每组20只。用药后7、14、21、28d大体观察及HE染色角膜新生血管情况，并使用RT-PCR方法检测各组角膜组织中VEGF的表达。结果 PEDF/CS纳米粒大部分呈规则的球型，粒径为（354±1.6）nm，zeta电位为（22.5±0.7）mV，包封率为（76.15±2.80）%，体系较为稳定，具有缓释功能。明显下调兔创伤角膜中的VEGF的表达促进角膜修复，各时间点各组间差异具有统计学意义（P＜0.01）。结论色素上皮衍生因子壳聚糖纳米粒滴眼液通过下调兔创伤角膜中的VEGF的表达，可以减少角膜新生血管的生成。
目的：應用離子交聯法製備色素上皮衍生因子殼聚糖（PEDF/CS）納米粒,攷察其體外性質及其在兔角膜堿燒傷中對角膜新生血管的影響，探討其作用機製。方法通過離子交聯法製備PEDF/CS納米粒，攷察納米粒粒徑、包封率、zeta電位及體外釋放量，建立兔角膜堿燒傷模型，右眼作為實驗眼。單純隨機抽樣法，分為生理鹽水對照組(A組)、PEDF滴眼液組(B組)、PEDF/CS納米粒組(C組)，每組20隻。用藥後7、14、21、28d大體觀察及HE染色角膜新生血管情況，併使用RT-PCR方法檢測各組角膜組織中VEGF的錶達。結果 PEDF/CS納米粒大部分呈規則的毬型，粒徑為（354±1.6）nm，zeta電位為（22.5±0.7）mV，包封率為（76.15±2.80）%，體繫較為穩定，具有緩釋功能。明顯下調兔創傷角膜中的VEGF的錶達促進角膜脩複，各時間點各組間差異具有統計學意義（P＜0.01）。結論色素上皮衍生因子殼聚糖納米粒滴眼液通過下調兔創傷角膜中的VEGF的錶達，可以減少角膜新生血管的生成。
목적：응용리자교련법제비색소상피연생인자각취당（PEDF/CS）납미립,고찰기체외성질급기재토각막감소상중대각막신생혈관적영향，탐토기작용궤제。방법통과리자교련법제비PEDF/CS납미립，고찰납미립립경、포봉솔、zeta전위급체외석방량，건립토각막감소상모형，우안작위실험안。단순수궤추양법，분위생리염수대조조(A조)、PEDF적안액조(B조)、PEDF/CS납미립조(C조)，매조20지。용약후7、14、21、28d대체관찰급HE염색각막신생혈관정황，병사용RT-PCR방법검측각조각막조직중VEGF적표체。결과 PEDF/CS납미립대부분정규칙적구형，립경위（354±1.6）nm，zeta전위위（22.5±0.7）mV，포봉솔위（76.15±2.80）%，체계교위은정，구유완석공능。명현하조토창상각막중적VEGF적표체촉진각막수복，각시간점각조간차이구유통계학의의（P＜0.01）。결론색소상피연생인자각취당납미립적안액통과하조토창상각막중적VEGF적표체，가이감소각막신생혈관적생성。
Objective To prepare the pigment epithelium-derived factor/chitosan (PEDF/CS) nanoparticles with lonic crosslinking method, to investigate its properties in vitro and its influence on corneal neovascularization in rabbit cornea alkali burn, and to discuss its mechanism. Methods PEDF/CS nanoparticles were prepared with lonic crosslinking method, the nanoparticle size, encapsulation rate, zeta-potential and drug release in vitro were investigated;rabbit cornea alkali burn models were built in 60 New Zealand rabbits, the right eyes were chosen to be the experimental eye; the 60 rabbits were randomly divided into 3 groups: group A (saline control group), group B (PEDF eye drop group) and group C ( PEDF/CS nanoparticles group), 20 rabbits in each;at the time points of 7, 14, 21 and 28 days after drug giving, corneal neovascularization was observed by HE staining and VEGF expression in cornea tissue in each group was detected by RT-PCR. Results Most PEDF/CS nanoparticles were in uniform sphere with the particle diameter of 354±1.6nm, zeta-potential of 22.5 ± 0.7mV and encapsulation rate of (76.15 ± 2.80)%, the system was relatively stable with sustained-releasing func-tion;VEGF expression was obviously down-regulated, which promoted the corneal tissue reparation;the differences among groups at each time point were of statistical significance (P<0.01). Conclusions PEDF/CS nanoparticle eye drop can reduce corneal neovascular-ization by down-regulating VEGF expression in rabbit injured cornea.
