食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
6期
2356-2360
,共5页
杨祖伟%陈晓霞%孙浩洋%游景水
楊祖偉%陳曉霞%孫浩洋%遊景水
양조위%진효하%손호양%유경수
羰基价%影响因素%2,4-二硝基苯肼
羰基價%影響因素%2,4-二硝基苯肼
탄기개%영향인소%2,4-이초기분정
carbonyl group value%influence factor%2,4-dinitrophenyl-hydrazine
目的：对羰基价测定影响因素进行考察。方法采用GB/T 5009.37-2003食用植物油卫生标准的分析方法,采用2,4-二硝基苯肼比色法,分别考察测定结果的精密度,显色液稳定性,不同浓度的三氯乙酸溶液、氢氧化钾溶液、2,4-二硝基苯肼溶液、无醛乙醇以及不同的水浴时间、温度对羰基价测定结果的影响。结果该方法的精密度为0.9%～2.4%,显色液在0~90 min内的吸光度呈较快的下降趋势,羰基价的测定结果会随三氯乙酸浓度(3.0~5.0 g/100 mL)、2,4-二硝基苯肼浓度(40~60 mg/100 mL)、水浴时间(20~40 min)、水浴温度(50~70℃)的增大而增大,会随氢氧化钾浓度(3.0~5.0 g/100 mL)的增大而降低、90%无醛乙醇会使显色液发生浑浊而无法测定。结论羰基价的检测应严格按照GB/T5009.37-2003中规定的条件进行测定。
目的：對羰基價測定影響因素進行攷察。方法採用GB/T 5009.37-2003食用植物油衛生標準的分析方法,採用2,4-二硝基苯肼比色法,分彆攷察測定結果的精密度,顯色液穩定性,不同濃度的三氯乙痠溶液、氫氧化鉀溶液、2,4-二硝基苯肼溶液、無醛乙醇以及不同的水浴時間、溫度對羰基價測定結果的影響。結果該方法的精密度為0.9%～2.4%,顯色液在0~90 min內的吸光度呈較快的下降趨勢,羰基價的測定結果會隨三氯乙痠濃度(3.0~5.0 g/100 mL)、2,4-二硝基苯肼濃度(40~60 mg/100 mL)、水浴時間(20~40 min)、水浴溫度(50~70℃)的增大而增大,會隨氫氧化鉀濃度(3.0~5.0 g/100 mL)的增大而降低、90%無醛乙醇會使顯色液髮生渾濁而無法測定。結論羰基價的檢測應嚴格按照GB/T5009.37-2003中規定的條件進行測定。
목적：대탄기개측정영향인소진행고찰。방법채용GB/T 5009.37-2003식용식물유위생표준적분석방법,채용2,4-이초기분정비색법,분별고찰측정결과적정밀도,현색액은정성,불동농도적삼록을산용액、경양화갑용액、2,4-이초기분정용액、무철을순이급불동적수욕시간、온도대탄기개측정결과적영향。결과해방법적정밀도위0.9%～2.4%,현색액재0~90 min내적흡광도정교쾌적하강추세,탄기개적측정결과회수삼록을산농도(3.0~5.0 g/100 mL)、2,4-이초기분정농도(40~60 mg/100 mL)、수욕시간(20~40 min)、수욕온도(50~70℃)적증대이증대,회수경양화갑농도(3.0~5.0 g/100 mL)적증대이강저、90%무철을순회사현색액발생혼탁이무법측정。결론탄기개적검측응엄격안조GB/T5009.37-2003중규정적조건진행측정。
Objective To investigate the impact factors in the measurement of carbonyl value. Methods The GB/T 5009.37-2003 Method for analysis of hygienic standard of edible oils was applied to determine the impact factors in the measurement of carbonyl value. The 2,4-dinitrophenylhydrazine colorimetric measurement was adopted, precision test and color liquid stability were studied, the impact of different concentrations of TCA solution, potassium hydroxide solution, 2,4-dinitrophenylhydrazine solution, aldehyde-free ethanol and different water bath time, temperature on carbonyl value were investigated. Result This method showed a good precision of 0.9%~2.4%, color absorbance showed a rapid decline in 90 min, carbonyl value would be increased when the impact factor of TCA concentration (3.0~5.0 g/100 mL), 2,4-dinitro-phenylhydrazine concentration (40~60 mg/100 mL), water bath time (20~40 min) and bath temperature (50~70 ℃) had raised. Meanwhile, carbonyl value decreased as the concentration of potassium hydroxide (3.0~5.0 g/100 mL) dropped, and 90% aldehyde-free ethanol made color liquid could not be measured due to the turbidity. Conclusion The detection of carbonyl value should be strictly carried out under the conditions prescribed in GB/T 5009.37-2003.
