CAJ | 학술논문

目的：分析BRAFV600E基因突变在非小细胞肺癌阳性胸水中的表达情况。方法将胸水内查见肺腺癌细胞的病例24例其阳性胸水离心沉渣制作细胞块。分离细胞块样品 DNA ，使用扩增突变系统（ARMS ）方法进行 PCR 扩增，检测BRAFV600E基因突变情况。结果检测成功率为100％，突变检出率为8％，检出突变2例，男性及女性各1例，FAM 信号Ct值分别为18.7及17.8，STD的 FAM 信号Ct值为18。结论可以应用非小细胞肺癌阳性胸水代替肺癌肿瘤组织检测BRA FV600E基因突变，对指导晚期非小细胞肺癌病人靶向药物治疗具有重要指导意义。
목적：분석BRAFV600E기인돌변재비소세포폐암양성흉수중적표체정황。방법장흉수내사견폐선암세포적병례24례기양성흉수리심침사제작세포괴。분리세포괴양품 DNA ，사용확증돌변계통（ARMS ）방법진행 PCR 확증，검측BRAFV600E기인돌변정황。결과검측성공솔위100％，돌변검출솔위8％，검출돌변2례，남성급녀성각1례，FAM 신호Ct치분별위18.7급17.8，STD적 FAM 신호Ct치위18。결론가이응용비소세포폐암양성흉수대체폐암종류조직검측BRA FV600E기인돌변，대지도만기비소세포폐암병인파향약물치료구유중요지도의의。
Objective To investigate the BRAFV600E mutation in positive pleural effusion of non‐small cell lung cancer (NSCLC) .Methods 24 patients with the pleural effusion including lung adenocarcinoma cancer cells were selected ,whose posi‐tive pleural effusion was made for cell blocks by centrifugal sediment .DNA in cell blocks was separated to amplify DNA by the method of amplification refractory mutation system (ARMS) and detect gene mutations of BRAFV600E .Results Test success rate was 100% and mutation detection rate was 8% .2 cases mutation were checked out ,of which men and women each 1 case . FAM signal Ct value were respectively 18.7 and 17.8 ,STD FAM signals of Ct value was 18 .Conclusion NSCLC positive pleu‐ral effusion could take the place of tumor tissue to detect BRAFV600E gene mutation ,which was obviously important to guide the targeted drugs in terminal NSCLC patients .