食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
6期
2391-2398
,共8页
钟佳耘%严洁%王娅%周忠良
鐘佳耘%嚴潔%王婭%週忠良
종가운%엄길%왕아%주충량
一次性竹筷%浸出液%斑马鱼%胚胎发育%畸形%仔鱼死亡率
一次性竹筷%浸齣液%斑馬魚%胚胎髮育%畸形%仔魚死亡率
일차성죽쾌%침출액%반마어%배태발육%기형%자어사망솔
disposable chopsticks%leachate%zebrafish%embryonic development%malformation%larvae mortality
目的：观察市售某合格品牌一次性竹筷浸出液对斑马鱼胚胎发育的毒性效应。方法将一次性竹筷称重,用过滤自来水浸泡24 h,配制5.0、7.5、11.2、16.8、25.0 mg/mL 5个不同浓度的一次性竹筷浸出液。选取成年健康斑马鱼将雌雄按1：2的比例进行交配产卵,分别用不同浓度的竹筷浸出液对受精卵(1枚受精卵/培养皿)进行暴露孵化实验,设空白对照。通过显微成像技术观察一次性竹筷浸出液暴露对斑马鱼胚胎发育形态、孵化率、仔鱼畸形率与仔鱼死亡率的影响。在总周期96 h内每隔12 h观察一次。结果对照组仔鱼以及浓度在11.2 mg/mL以下的各浸筷液组仔鱼无畸形,无死亡。高浓度浸出液(16.8 mg/mL和25 mg/mL)中孵化的斑马鱼表现出胚胎发育明显延迟。虽然96 h 总孵化率在不同组间无差异,但是高浓度竹筷浸出液中孵出的斑马鱼仔鱼出现高度畸形,96 h 仔鱼畸形率分别为67.65%和61.29%；仔鱼死亡率的增加呈现剂量依赖性,分别为61.76%和100%。结论市售某合格品牌一次性竹筷浸出液可导致斑马鱼胚胎发育延迟、仔鱼畸形和早期死亡。
目的：觀察市售某閤格品牌一次性竹筷浸齣液對斑馬魚胚胎髮育的毒性效應。方法將一次性竹筷稱重,用過濾自來水浸泡24 h,配製5.0、7.5、11.2、16.8、25.0 mg/mL 5箇不同濃度的一次性竹筷浸齣液。選取成年健康斑馬魚將雌雄按1：2的比例進行交配產卵,分彆用不同濃度的竹筷浸齣液對受精卵(1枚受精卵/培養皿)進行暴露孵化實驗,設空白對照。通過顯微成像技術觀察一次性竹筷浸齣液暴露對斑馬魚胚胎髮育形態、孵化率、仔魚畸形率與仔魚死亡率的影響。在總週期96 h內每隔12 h觀察一次。結果對照組仔魚以及濃度在11.2 mg/mL以下的各浸筷液組仔魚無畸形,無死亡。高濃度浸齣液(16.8 mg/mL和25 mg/mL)中孵化的斑馬魚錶現齣胚胎髮育明顯延遲。雖然96 h 總孵化率在不同組間無差異,但是高濃度竹筷浸齣液中孵齣的斑馬魚仔魚齣現高度畸形,96 h 仔魚畸形率分彆為67.65%和61.29%；仔魚死亡率的增加呈現劑量依賴性,分彆為61.76%和100%。結論市售某閤格品牌一次性竹筷浸齣液可導緻斑馬魚胚胎髮育延遲、仔魚畸形和早期死亡。
목적：관찰시수모합격품패일차성죽쾌침출액대반마어배태발육적독성효응。방법장일차성죽쾌칭중,용과려자래수침포24 h,배제5.0、7.5、11.2、16.8、25.0 mg/mL 5개불동농도적일차성죽쾌침출액。선취성년건강반마어장자웅안1：2적비례진행교배산란,분별용불동농도적죽쾌침출액대수정란(1매수정란/배양명)진행폭로부화실험,설공백대조。통과현미성상기술관찰일차성죽쾌침출액폭로대반마어배태발육형태、부화솔、자어기형솔여자어사망솔적영향。재총주기96 h내매격12 h관찰일차。결과대조조자어이급농도재11.2 mg/mL이하적각침쾌액조자어무기형,무사망。고농도침출액(16.8 mg/mL화25 mg/mL)중부화적반마어표현출배태발육명현연지。수연96 h 총부화솔재불동조간무차이,단시고농도죽쾌침출액중부출적반마어자어출현고도기형,96 h 자어기형솔분별위67.65%화61.29%；자어사망솔적증가정현제량의뢰성,분별위61.76%화100%。결론시수모합격품패일차성죽쾌침출액가도치반마어배태발육연지、자어기형화조기사망。
ABSTRACT:Objective To observe the toxic effects of the leachate of qualified disposable chopsticks from commercially available products on the embryonic development in zebrafish (Danio rerio). Methods The bamboo chopsticks were weighted, soaked in filtered tap water for 24 h to make the leachate of disposable chopsticks of 5 different concentrations: 5.0, 7.5, 11.2 16.8 and 25.0 mg/mL. Healthy adult parent zebrafish were chosen (female:male=1:2) to mate and the fertilized eggs were hatched (1 egg/petri dish) in different concentrations of chopsticks leachate to conduct the exposure experiment. The microscopic imaging technique was used to observe the effect of disposable chopsticks’ leachate on embryo development, hatchability, larvae malformation and mortality. Each egg was observed every 12 h for a total period of 96 h. Results There was no malformation and death in control group and leachate groups under 11.2 mg/mL. Zebrafish hatching in high concentration of leachate (16.8 mg/mL and 25 mg/mL) showed significantly delayed embryonic development. Although there was no difference in total hatchability rate at 96 h among different groups, highly deformed were found in the zebrafish larvae hatched in high concentration of chopsticks leachate. The malformation rates at 96 h in group 16.8 mg/mL and group 25 mg/mL were 67.65%and 61.29%, respectively. The larvae mortality was increased dose-dependently with 61.76% and 100% respectively. Conclusion The leachate from the commercially available disposable chopsticks of certain qualified brand could result in delayed embryo development, malformation and early death of zebrafish larvae.
