食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
6期
2337-2341
,共5页
反相高效液相色谱法%超高效液相色谱法%饮料%咖啡因
反相高效液相色譜法%超高效液相色譜法%飲料%咖啡因
반상고효액상색보법%초고효액상색보법%음료%가배인
reversed high performance liquid chromatography%ultra-high performance liquid chromatography%beverages%caffeine
目的：对原反相高效液相色谱(RP-HPLC)方法进行转化并优化，建立超高效液相色谱(UPLC)方法，为饮料中咖啡因的含量测定提供更快速、高效、灵敏和环保的方法。方法分别采用RP-HPLC法和UPLC法测定饮料中咖啡因的含量，并对比二者的精密度、稳定性、重复性和加标回收率。结果2种方法所得8种市售饮料中咖啡因的含量测定结果变化趋势一致，但 UPLC法含量测定结果比RP-HPLC法含量测定结果偏高，二者精密度、回收率均符合要求。结论 UPLC 法可以成功替代 RP-HPLC 法，为饮料中咖啡因的快速定性定量分析与检测提供新的途径。
目的：對原反相高效液相色譜(RP-HPLC)方法進行轉化併優化，建立超高效液相色譜(UPLC)方法，為飲料中咖啡因的含量測定提供更快速、高效、靈敏和環保的方法。方法分彆採用RP-HPLC法和UPLC法測定飲料中咖啡因的含量，併對比二者的精密度、穩定性、重複性和加標迴收率。結果2種方法所得8種市售飲料中咖啡因的含量測定結果變化趨勢一緻，但 UPLC法含量測定結果比RP-HPLC法含量測定結果偏高，二者精密度、迴收率均符閤要求。結論 UPLC 法可以成功替代 RP-HPLC 法，為飲料中咖啡因的快速定性定量分析與檢測提供新的途徑。
목적：대원반상고효액상색보(RP-HPLC)방법진행전화병우화，건립초고효액상색보(UPLC)방법，위음료중가배인적함량측정제공경쾌속、고효、령민화배보적방법。방법분별채용RP-HPLC법화UPLC법측정음료중가배인적함량，병대비이자적정밀도、은정성、중복성화가표회수솔。결과2충방법소득8충시수음료중가배인적함량측정결과변화추세일치，단 UPLC법함량측정결과비RP-HPLC법함량측정결과편고，이자정밀도、회수솔균부합요구。결론 UPLC 법가이성공체대 RP-HPLC 법，위음료중가배인적쾌속정성정량분석여검측제공신적도경。
Objective To establish an ultra performance liquid chromatography (UPLC) method to replace the reversed high performance liquid chromatography (RP-HPLC) for a faster, more sensitive and more environmental friendly detection for caffeine content in beverages. Methods Both RP-HPLC and UPLC methods were used in the determination of caffeine content in 8 kinds of beverages, and the precision, stability, repeatability and recovery rates of the 2 methods were compared. Results Caffeine content determined in 8 beverages by the 2 methods had a similar trend, but the content of caffeine determined by UPLC was higher than that by RP-HPLC. Both methods showed good precision and recovery rates. Conclusion UPLC method as a new detection method can replace RP-HPLC in the quantitative analysis of caffeine in beverages.
