CAJ | 학술논문

目的：建立检测分析水产品中氯霉素残留量的气相色谱-串联质谱联用(GC-MS/MS)的方法。方法样品采用乙酸乙酯提取、经过C18固相萃取柱净化，用硅烷化试剂衍生化，采用GC-MS/MS分析时，氯霉素在15 min内流出。结果对样品添加两种浓度的加标质控，回收率在80%以上，相对标准偏差(RSD)在6.2%以内。在0.5~10 mg/L质量浓度范围内呈线性关系，相关系数大于0.99。结论该方法可用于氯霉素残留的准确定量分析，灵敏度、精密度和准确度均能满足渔药残留检测分析要求。
목적：건립검측분석수산품중록매소잔류량적기상색보-천련질보련용(GC-MS/MS)적방법。방법양품채용을산을지제취、경과C18고상췌취주정화，용규완화시제연생화，채용GC-MS/MS분석시，록매소재15 min내류출。결과대양품첨가량충농도적가표질공，회수솔재80%이상，상대표준편차(RSD)재6.2%이내。재0.5~10 mg/L질량농도범위내정선성관계，상관계수대우0.99。결론해방법가용우록매소잔류적준학정량분석，령민도、정밀도화준학도균능만족어약잔류검측분석요구。
ABSTRACT:Objective To establish a determination method of chloramphenicol (CAP) in aquatic products by gas chromatography-tandem mass spectrometry (GC-MS/MS). Methods The samples were extracted by ethyl acetate, cleaned up by C18 SPE column, and derived with silanization reagent, then the extracts were determined by MS/MS detector based on SCAN and MRM mode. Results CAP was well separated within 15 min. This method showed that the spiked average recoveries above 80%, the RSD ranged within 6.2%, and the calibration of those pesticides were linearity within the range of 0.5~10 mg/L (r2>0.99). Conclusion The method is accurate and sensitive, it is suitable for analysis of CAP residues in aquatic products.