CAJ | 학술논문

目的：分析我国2012年腹水标本来源细菌分布及抗菌药敏感状况。方法2012年从557家三级医院腹水标本中分离细菌，常规方法培养分离腹水标本中的细菌，用纸片法、微量稀释法或 E －test 法测定细菌药物敏感性。结果共分离出10252株细菌，排在前5位的分别是大肠埃希菌（3215株，31.4％），肺炎克雷伯菌（860株，8.4％），屎肠球菌（703株，6.9％），铜绿假单胞菌（548株，5.3％）和表皮葡萄球菌（531株，5.2％）。金黄色葡萄球菌对苯唑西林耐药率为47.4％。未发现对万古霉素和利奈唑胺耐药的葡萄球菌。粪肠球菌和屎肠球菌对万古霉素耐药率分别为1.4％和2.5％。大肠埃希菌、肺炎克雷伯菌和阴沟肠杆菌对头孢曲松的敏感率分别为28.2％，50.8％和41.0％；对美罗培南的敏感率分别为96.7％，88.8％和96.5％。铜绿假单胞菌对碳青霉烯类、头孢他啶和头孢吡肟的敏感率超过60％，对喹诺酮类药物敏感率超过75％。鲍曼不动杆菌对碳青霉烯类药敏感率低于40％，对阿米卡星敏感率为50.8％。结论三级医院患者腹水标本分离细菌中最常见病原菌为大肠埃希菌和肺炎克雷伯菌，不同病原菌对抗菌药敏感性不同。
목적：분석아국2012년복수표본래원세균분포급항균약민감상황。방법2012년종557가삼급의원복수표본중분리세균，상규방법배양분리복수표본중적세균，용지편법、미량희석법혹 E －test 법측정세균약물민감성。결과공분리출10252주세균，배재전5위적분별시대장애희균（3215주，31.4％），폐염극뢰백균（860주，8.4％），시장구균（703주，6.9％），동록가단포균（548주，5.3％）화표피포도구균（531주，5.2％）。금황색포도구균대분서서림내약솔위47.4％。미발현대만고매소화리내서알내약적포도구균。분장구균화시장구균대만고매소내약솔분별위1.4％화2.5％。대장애희균、폐염극뢰백균화음구장간균대두포곡송적민감솔분별위28.2％，50.8％화41.0％；대미라배남적민감솔분별위96.7％，88.8％화96.5％。동록가단포균대탄청매희류、두포타정화두포필우적민감솔초과60％，대규낙동류약물민감솔초과75％。포만불동간균대탄청매희류약민감솔저우40％，대아미잡성민감솔위50.8％。결론삼급의원환자복수표본분리세균중최상견병원균위대장애희균화폐염극뢰백균，불동병원균대항균약민감성불동。
Objective To investigate the bacterial distribution and resis-tance to antibiotic isolated from peritoneal fluid in China, 2012.Methods The bacteria isolated from peritoneal fluid in the tertiary hospitals were routinely identified.Disc diffusion test, minimun ihibitory concentration ( MIC) test and E-test were used to detect the antimicro-bial sensitivi-ty.Results Total of 10252 pathogenic strains were collec-ted from peri-toneal fluid specimens from 557 tertiary hospitals, which included Esche-richia coli 3215 strains ( 31.4%) , Klebsiella pneumoniae 860 strains ( 8.4%) , Enterococcus faecium 730 strains ( 6.9%) , Pseu-domonas aeruginosa 548 strains ( 5.3%) and Staphylococcus epidermidis 531 strains (5.2%).The resistance rate of Staphylococcus aureus strains to oxacillin were 47.4%, no vancomycin and linezolide resistant isolate were found.The rates of susceptibility of Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacaeisolates to ceftriaxone were 28.2%, 50.8%and 41.0%, respectively;to meropenem were 96.7%, 88.8%and 96.5%, respectively.The rates of susceptibility of Pseudomonas aeruginosa to carbapenem, ceftazidime and cefepime were >60%, to quinolone was >75%.The rates of susceptibility of Acinetobacter baumannii isolates to cabarpenem were <40%, to amikacin were 50.8%.Conclusions The two most common species from peritoneal fluid in tertiary hospital were Escherichia coli and Klebsiella pneumonia.There were some difference in bacterial resistance to different antibiotics.