CAJ | 학술논문

目的：研究烯酰吗啉在橙子和芹菜中的基质效应，以准确测定橙子和芹菜中的烯酰吗啉。方法利用超高效液相色谱-串联质谱法进行测定。橙子和芹菜样品利用乙腈提取后直接进样分析，并采用正离子多反应监测模式，外标法定量，比较了甲醇-水、甲醇-0.1%(V：V)甲酸水溶液、甲醇-1 mmol/L乙酸铵水溶液3种流动相组成对烯酰吗啉基质效应的影响情况。同时也比较了纯甲醇溶剂和样品空白基质配制的标准对烯酰吗啉基质效应的影响。结果利用样品空白基质配制的标准能够有效地抑制烯酰吗啉的基质效应，而测定橙子和芹菜基质中烯酰吗啉时，使用甲醇-0.1%(V：V)甲酸水溶液作为流动相，可在一定程度上抑制烯酰吗啉的基质效应。结论本方法快速简便，为烯酰吗啉在其他果蔬中的基质效应研究提供参考。
목적：연구희선마람재등자화근채중적기질효응，이준학측정등자화근채중적희선마람。방법이용초고효액상색보-천련질보법진행측정。등자화근채양품이용을정제취후직접진양분석，병채용정리자다반응감측모식，외표법정량，비교료갑순-수、갑순-0.1%(V：V)갑산수용액、갑순-1 mmol/L을산안수용액3충류동상조성대희선마람기질효응적영향정황。동시야비교료순갑순용제화양품공백기질배제적표준대희선마람기질효응적영향。결과이용양품공백기질배제적표준능구유효지억제희선마람적기질효응，이측정등자화근채기질중희선마람시，사용갑순-0.1%(V：V)갑산수용액작위류동상，가재일정정도상억제희선마람적기질효응。결론본방법쾌속간편，위희선마람재기타과소중적기질효응연구제공삼고。
Objective To increase the accuracy of dimethomorph detection in orange and celery by studying the matrix effect. Methods The detection were conducted on an ultra performance liquid chromatography-tandem mass spectrometry, the effect of 3 kinds of mobile phases including methanol-water, methanol-0.1% (V:V) formic acid solution and methanol-1 mmol/L ammonium acetate solution on the matrix effects were compared. Dimethomorph in orange and celery samples was extracted by acetonitrile. Positive ion mode and multiple reaction monitoring were used in the analysis and external standard method was used for quantification. Also, the effect of preparation methods of standard solutions including diluted with methanol and blank matrix were studied. Results The matrix effects of dimethomorph were effectively inhibited when the standard solution was diluted with blank matrix, and the matrix effects of dimethomorph were partly inhibited when the mobile phase was methanol-0.1%(V:V) formic acid solution in determination of dimethomorph in oranges and celery. Conclusions This method is simple and fast, which can provide a reference for the study of matrix effects of dimethomorph in other fruits and vegetables.