CAJ | 학술논문

目的：探讨姜黄素预处理对小鼠急性脊髓损伤后神经功能的保护作用及其可能机制。方法小鼠随机分为4组，假手术组、模型组、对照组（甲强龙，40 mg· kg－1）和实验组（姜黄素，200 mg· kg－1）。造模前30 min，分别腹腔注射相应药物，用改良的Allen′s 法建立急性脊髓损伤模型后，进行行为学评价，用Western blot法测定脊髓核因子E2相关因子2（Nrf2）蛋白表达，用酶联免疫吸附法（ELISA）测定血清肿瘤坏死因子α（TNF－α）和白细胞介素6（IL－6）的表达。结果与模型组比较，实验组小鼠的后肢功能及斜板实验评分显著增加，Nrf2和TNF－α表达均明显改善，IL－6表达在8 h减少，但之后的时间点无明显差别。结论预防性给予姜黄素，可以通过上调Nrf2、下调炎症因子对小鼠起到神经功能的保护作用。
목적：탐토강황소예처리대소서급성척수손상후신경공능적보호작용급기가능궤제。방법소서수궤분위4조，가수술조、모형조、대조조（갑강룡，40 mg· kg－1）화실험조（강황소，200 mg· kg－1）。조모전30 min，분별복강주사상응약물，용개량적Allen′s 법건립급성척수손상모형후，진행행위학평개，용Western blot법측정척수핵인자E2상관인자2（Nrf2）단백표체，용매련면역흡부법（ELISA）측정혈청종류배사인자α（TNF－α）화백세포개소6（IL－6）적표체。결과여모형조비교，실험조소서적후지공능급사판실험평분현저증가，Nrf2화TNF－α표체균명현개선，IL－6표체재8 h감소，단지후적시간점무명현차별。결론예방성급여강황소，가이통과상조Nrf2、하조염증인자대소서기도신경공능적보호작용。
Objective To explore the effect and mechanism of curcumin on neuronal protective function in acute spinal cord model ( ASCI) mice. Methods Divide healthy and clean class mice into sham -operated group,model group, control group ( methylprednisolone, 40 mg · kg-1 ) and treatment group ( curcumin 200 mg · kg-1 ).Each mice respectively received injection in intraperitoneal with drugs at 30 min before the sur-gery.Then, experimental animal models was established following im-proved Allen′s model.The behavior of the model mice was performed.The nuclear factor E2-related faetor 2 ( Nrf 2 ) antibody expression was examined with Western blot.And expression of tumor necrosis factor-α( TNF-α) and interleukin-6 ( IL-6 ) were examined with enzyme-linked immunosorbent assay.Results Compared to model group, beha-vior score,neurologic function,the expression of Nrf 2 and TNF -αin treatment group achieved significant improvement.The IL-6 expression in treatment group only revealed weaker in 8 h, but no notable difference compared to model group after 8 h.Conclusion The preliminary study shows that the way of curcumin preconditioning to improve neurologic function may by decreasing inflammatory factor and raising the expression of Nrf2.