CAJ | 학술논문

目的：观察川芎嗪联合顺铂对Lewis肺腺癌小鼠瘤组织中的缺氧诱导因子1α（ HIF－1α）、碱性成纤维细胞生长因子（ b －FGF ）、血管生成抑制蛋白（ VASH－1）变化并探讨其抗肿瘤机制。方法将48只小鼠模型随机分成4组，对照组、顺铂组（2 mg· kg－1）、川芎嗪组（100 mg· kg－1）和联合组（顺铂组2 mg· kg－1＋川芎嗪组100 mg· kg－1），每组12只。给药2周，处死小鼠，称量瘤重，计算抑瘤率；用免疫组化法检测各组HIF－1α、b－FGF、VASH－1的表达。结果与对照组相比，实验组的抑瘤率均明显提高（P＜0.05），联合组抑瘤率最高；与对照组相比，3个给药组的HIF－1α、b－FGF、VASH－1表达均明显降低（P＜0.05）。 HIF－1α与b－FGF成正相关（P＜0.05），b－FGF与VASH－1成正相关（P＜0.05），HIF－1α与VASH－1无相关。结论川芎嗪联合顺铂降低HIF－1α的表达，进而降低b－FGF与VASH－1的表达，协同抑制肿瘤生长。
목적：관찰천궁진연합순박대Lewis폐선암소서류조직중적결양유도인자1α（ HIF－1α）、감성성섬유세포생장인자（ b －FGF ）、혈관생성억제단백（ VASH－1）변화병탐토기항종류궤제。방법장48지소서모형수궤분성4조，대조조、순박조（2 mg· kg－1）、천궁진조（100 mg· kg－1）화연합조（순박조2 mg· kg－1＋천궁진조100 mg· kg－1），매조12지。급약2주，처사소서，칭량류중，계산억류솔；용면역조화법검측각조HIF－1α、b－FGF、VASH－1적표체。결과여대조조상비，실험조적억류솔균명현제고（P＜0.05），연합조억류솔최고；여대조조상비，3개급약조적HIF－1α、b－FGF、VASH－1표체균명현강저（P＜0.05）。 HIF－1α여b－FGF성정상관（P＜0.05），b－FGF여VASH－1성정상관（P＜0.05），HIF－1α여VASH－1무상관。결론천궁진연합순박강저HIF－1α적표체，진이강저b－FGF여VASH－1적표체，협동억제종류생장。
Objective To observe the effect of Tetramethylpyrazine combined with cisplatin on Lewis lung adenocarcinoma by the change of hypoxia-inducible factor 1 alpha ( HIF -1α) , basic fibroblast growth factorb ( b-FGF) ,vasohibin-1 ( VASH-1 ) in mice tumor tissues and explore the mechanism of anti -tumor.Methods Forty -eight mice were randomly divided into 4 groups, control group, cisplatin group, Tetramethylpyrazine group and combined group, 12 rats in each group, cisplatin at a dose of 2 mg· kg -1 , Tetramethylpyrazine at a dose of 100 mg· kg-1 , combination group( the doses as above).All mice were sac-rificed after treatment for two weeks and these tumors were obtained.The weighed, and the tumor inhibitory was calculated.The expressions of HIF-1α, b -FGF and VASH -1 were determined by immunohisto-chemistry after two weeks administration.Results Compared with the control group, the tumor inhibition rate in experimental groups was signi-ficantly increased ( P<0.05 ) , and was highest in combination group.The expression of HIF -1α, b -FGF and VASH -1 in the cisplatin group, Tetramethylpyrazine group and combined group were decreased obviously compared with control group( P<0.05).And the levels of the expression were the lowest in the combined group and the highest in control group.HIF -1αis positively correlated with b -FGF ( P <0.05 ) , b -FGF is positively correlated with VASH-1 ( P <0.05 ) , HIF-1αis uncorrelated with VASH-1.Conclusion Tetramethylpyrazine combined with cisplatin decreased the expression of HIF-1α, thereby reducing the expression of b-FGF and VASH-1 , inhibiting the tumor growth synergistically.