食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
6期
2297-2302
,共6页
李平%谢体波%易重任%魏丽丽%王大敏%张建宇%欧翔%陈芳%罗先坤
李平%謝體波%易重任%魏麗麗%王大敏%張建宇%歐翔%陳芳%囉先坤
리평%사체파%역중임%위려려%왕대민%장건우%구상%진방%라선곤
黄曲霉毒素M1%酶联免疫法%高效液相色谱法%牛奶
黃麯黴毒素M1%酶聯免疫法%高效液相色譜法%牛奶
황곡매독소M1%매련면역법%고효액상색보법%우내
aflatoxin M1%enzyme linked immune sorbent assay%high performance liquid chromatography%milk
目的:为验证本公司生产的黄曲霉毒素 M1酶联免疫法(ELISA)检测试剂盒的检测效果。方法用ELISA检测试剂盒对牛奶、酸奶、奶粉和干酪等4种样品中黄曲霉毒素M1的残留量进行检测,并与高效液相色谱荧光检测法进行对照。结果该试剂盒对4种样品的最低检测限分别为0.039、0.192、0.306、0.199μg/kg(L),试剂盒板内板间变异系数均小于5%;对4种样品做加标回收试验,其回收率均在93%~120%之间,变异系数均小于10%;该方法与高效液相色谱法检测实际样品的阴、阳性判断结果一致。结论该方法稳定、可靠、灵敏,可满足食品中黄曲霉毒素M1残留快速检测的需要。
目的:為驗證本公司生產的黃麯黴毒素 M1酶聯免疫法(ELISA)檢測試劑盒的檢測效果。方法用ELISA檢測試劑盒對牛奶、痠奶、奶粉和榦酪等4種樣品中黃麯黴毒素M1的殘留量進行檢測,併與高效液相色譜熒光檢測法進行對照。結果該試劑盒對4種樣品的最低檢測限分彆為0.039、0.192、0.306、0.199μg/kg(L),試劑盒闆內闆間變異繫數均小于5%;對4種樣品做加標迴收試驗,其迴收率均在93%~120%之間,變異繫數均小于10%;該方法與高效液相色譜法檢測實際樣品的陰、暘性判斷結果一緻。結論該方法穩定、可靠、靈敏,可滿足食品中黃麯黴毒素M1殘留快速檢測的需要。
목적:위험증본공사생산적황곡매독소 M1매련면역법(ELISA)검측시제합적검측효과。방법용ELISA검측시제합대우내、산내、내분화간락등4충양품중황곡매독소M1적잔류량진행검측,병여고효액상색보형광검측법진행대조。결과해시제합대4충양품적최저검측한분별위0.039、0.192、0.306、0.199μg/kg(L),시제합판내판간변이계수균소우5%;대4충양품주가표회수시험,기회수솔균재93%~120%지간,변이계수균소우10%;해방법여고효액상색보법검측실제양품적음、양성판단결과일치。결론해방법은정、가고、령민,가만족식품중황곡매독소M1잔류쾌속검측적수요。
Objective To verify the test effect of aflatoxin M1 enzyme-linked immune sorbent assay (ELISA) detection kit developed by Beijing Kwinbon biotechnology company. Methods The method of ELISA was used to detect the residues of aflatoxin M1 in milk, yoghurt, milk powder and cheese, and compared with high performance liquid chromatography (HPLC). Results The results showed that the minimum detection limitation of the kit for the 4 kinds of samples were 0.039, 0.192, 0.306, and 0.199 μg/kg (L), the coefficient of variation was less than 5%. The recovery of the method was in the range of 93%~120%, and the coefficient of variation was less than 10%. The judgments of positive and negative by ELISA were the same as those of HPLC. Conclusion This method was efficient, reliable and sensitive, and could meet the fast-test for the determination of aflatoxin M1 residues in food.