食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
6期
2288-2296
,共9页
李越山%徐艳阳%孙永薇%史爱莹%李楠
李越山%徐豔暘%孫永薇%史愛瑩%李楠
리월산%서염양%손영미%사애형%리남
黑豆%酯酶%氧乐果%检测%响应面法
黑豆%酯酶%氧樂果%檢測%響應麵法
흑두%지매%양악과%검측%향응면법
black soybean%esterase%omethoate%detection%response surface methodology
目的：研究以氧乐果为代表的有机磷农药残留检测条件。方法以黑豆酯酶为酶源,α-乙酸萘酯为底物,采用酶抑制法,考察黑豆酯酶稀释倍数、显色时间、反应温度3个因素对黑豆酯酶活力测定的影响。然后通过3因素3水平响应面法优化黑豆酯酶活力的测定条件,并确定氧乐果对黑豆酯酶的最佳抑制时间和检测的灵敏度。结果当黑豆酯酶稀释倍数为18,显色反应时间为10 min,反应温度为41℃时,黑豆酯酶活力测定的吸光度值达到0.811。氧乐果抑制黑豆酯酶的最佳时间是4 min。绘制氧乐果对黑豆酯酶的标准曲线,得到最低检测限为0.1μg/mL。结论本研究获得了以黑豆酯酶抑制法检测氧乐果残留的最佳条件,其检测限低于有机磷农药氧乐果残留检测的最低限值要求(0.2μg/mL)。因此,以黑豆酯酶为酶源的酶抑制法可以作为有机磷农药氧乐果残留快速检测的一种有效手段。
目的：研究以氧樂果為代錶的有機燐農藥殘留檢測條件。方法以黑豆酯酶為酶源,α-乙痠萘酯為底物,採用酶抑製法,攷察黑豆酯酶稀釋倍數、顯色時間、反應溫度3箇因素對黑豆酯酶活力測定的影響。然後通過3因素3水平響應麵法優化黑豆酯酶活力的測定條件,併確定氧樂果對黑豆酯酶的最佳抑製時間和檢測的靈敏度。結果噹黑豆酯酶稀釋倍數為18,顯色反應時間為10 min,反應溫度為41℃時,黑豆酯酶活力測定的吸光度值達到0.811。氧樂果抑製黑豆酯酶的最佳時間是4 min。繪製氧樂果對黑豆酯酶的標準麯線,得到最低檢測限為0.1μg/mL。結論本研究穫得瞭以黑豆酯酶抑製法檢測氧樂果殘留的最佳條件,其檢測限低于有機燐農藥氧樂果殘留檢測的最低限值要求(0.2μg/mL)。因此,以黑豆酯酶為酶源的酶抑製法可以作為有機燐農藥氧樂果殘留快速檢測的一種有效手段。
목적：연구이양악과위대표적유궤린농약잔류검측조건。방법이흑두지매위매원,α-을산내지위저물,채용매억제법,고찰흑두지매희석배수、현색시간、반응온도3개인소대흑두지매활력측정적영향。연후통과3인소3수평향응면법우화흑두지매활력적측정조건,병학정양악과대흑두지매적최가억제시간화검측적령민도。결과당흑두지매희석배수위18,현색반응시간위10 min,반응온도위41℃시,흑두지매활력측정적흡광도치체도0.811。양악과억제흑두지매적최가시간시4 min。회제양악과대흑두지매적표준곡선,득도최저검측한위0.1μg/mL。결론본연구획득료이흑두지매억제법검측양악과잔류적최가조건,기검측한저우유궤린농약양악과잔류검측적최저한치요구(0.2μg/mL)。인차,이흑두지매위매원적매억제법가이작위유궤린농약양악과잔류쾌속검측적일충유효수단。
Objective To discuss detection conditions of omethoate residue, which is one of the most frequently-used organophosphorus pesticides. Methods Enzymatic inhibition method was applied and black soybean esterase was chosen as enzymatic resource, andα-naphthyl acetate as the substrate. Effects of dilution ratio of black soybean esterase, reaction time and reaction temperature on the determination of black soybean esterase activity were studied. Then optimum determination conditions, including the optimal inhibition period and detection sensitivity, were obtained by three-factor and three-level response surface methodology. Results The best condition was as follows: dilution ratio was 18 times, reaction time was 10 min and reaction temperature was 41℃. Under these conditions, the optimum absolution density reached 0.811. The optimal inhibition period was 4 min. Standard curve of inhibition of omethoate to black soybeans esterase was ploted, and the lowest detection limit was 0.1μg/mL, which was lower than the limit value of detection standard for organophosphorus pesticides residues. Conclusion The optimal detection conditions of omethoate residues for black soybean esterase were obtained, and the detection limit was lower than that (0.2 μg/mL) of organophosphorus pesticide residues detection requirements. The enzyme inhibition method based on black soybean esterase can be an effective method of rapid detection for organophosphorus pesticides residues since its lowest detection limit meets the threshold limit value requirement.
