CAJ | 학술논문

以自制的小型单电渗析装置于实验室制备氨基葡萄糖。经不同时间存放后氨基葡萄糖（Glucosamine, G-NH2）发生褐变反应，分别对无色、淡黄色、黑色的G-NH2进行抗氧化性实验。结果发现，G-NH2随着存放时间的延长颜色加深，其1,1-二苯基-2-三硝基苯肼（1,6-Bis(diphenylphosphino)hexane, DPPH）清除能力大幅增强，5 mg/mL时DPPH清除能力达到峰值97.09%；同样其还原力也随之增强，1 mg/mL浓度的G-NH2还原铁氰化钾在700 nm波长下的吸光值由0.566上升到1.9698，是同浓度谷胱甘肽（Glutathione, GSH）相应值的2.8倍；而·OH清除能力随着G-NH2颜色的加深急剧下降，且具有一定浓度依赖性，实验浓度为5 mg/mL时，无色G-NH2清除·OH能力为74.06%，淡黄色G-NH2清除率为16.46%，呈现黑色时已不具备·OH清除能力。
이자제적소형단전삼석장치우실험실제비안기포도당。경불동시간존방후안기포도당（Glucosamine, G-NH2）발생갈변반응，분별대무색、담황색、흑색적G-NH2진행항양화성실험。결과발현，G-NH2수착존방시간적연장안색가심，기1,1-이분기-2-삼초기분정（1,6-Bis(diphenylphosphino)hexane, DPPH）청제능력대폭증강，5 mg/mL시DPPH청제능력체도봉치97.09%；동양기환원력야수지증강，1 mg/mL농도적G-NH2환원철청화갑재700 nm파장하적흡광치유0.566상승도1.9698，시동농도곡광감태（Glutathione, GSH）상응치적2.8배；이·OH청제능력수착G-NH2안색적가심급극하강，차구유일정농도의뢰성，실험농도위5 mg/mL시，무색G-NH2청제·OH능력위74.06%，담황색G-NH2청제솔위16.46%，정현흑색시이불구비·OH청제능력。
Glucosamine was prepared by minitype electrodialyzer designed and produced in laboratory. Natural oxidation products of glucosamine were prepared by storing G-NH2 in the same environment with different periods of time. The reaction changed G-NH2 from colorless to yellow, even black. Antioxidant experiment results suggested that G-NH2’s ability of scavenging the DPPH free radicals increased over time and reached to 97.01%at the concentration of 5 mg/mL. Its ability of reducing was strengthened. With the concentration of 1 mg/mL, A700 nm wavelength absorbance value increased from 0.566 to 1.9698, which was 2.8 times of that of the same concentration of GSH. However, the scavenging activities of hydroxyl free radical fell sharply with the deepening of G-NH2 color, in a concentration dependent manner. The scavenging activities of hydroxyl free radical of colorless G-NH2 at 5 mg/mL was 74.06%, the pale yellow’s was 16.46%, the black’s had no function.