中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2015年
6期
421-425
,共5页
李润祥%冯承恩%梁碧华%田歆%朱慧兰
李潤祥%馮承恩%樑碧華%田歆%硃慧蘭
리윤상%풍승은%량벽화%전흠%주혜란
荨麻疹%疾病模型,动物%小鼠%皮炎,变应性接触性%二硝基苯类%二硝基氟苯
蕁痳疹%疾病模型,動物%小鼠%皮炎,變應性接觸性%二硝基苯類%二硝基氟苯
담마진%질병모형,동물%소서%피염,변응성접촉성%이초기분류%이초기불분
Urticaria%Disease models,animal%Mice%Dermatitis,allergic contact%Dinitrobenzenes%Dinitro-chlorobenzene
目的 建立小鼠免疫性接触性荨麻疹动物模型.方法 将60只BALB/c小鼠随机分5组:抗二硝基苯酚IgE单克隆抗体(抗DNP IgE)+二硝基氟苯(DNFB)组和抗DNP IgE+偏苯三酸酐(TMA)组小鼠尾静脉注射抗DNP IgE,24 h后分别在小鼠耳部涂DNFB和TMA;DNFB组、TMA组、生理氯化钠液(NS)组小鼠尾静脉注射NS,24 h后分别在小鼠双耳涂DNFB、TMA、NS,观察小鼠在14d内的风团、瘙痒情况、肥大细胞脱颗粒数、脾脏指数和皮肤病理.结果 抗DNP IgE+ DNFB组全部(12/12)和抗DNP IgE+ TMA组部分小鼠(8/12)产生风团;抗DNP IgE+ DNFB组及抗DNP IgE+ TMA组小鼠的搔抓次数[30 min时分别为(31.58±3.58)次/h,(22.17±3.81)次/h]、肥大细胞脱颗粒率[(70.21±26.01)％,(54.25±39.57)％]及脾脏指数(7.54±1.56,7.87±1.18)与NS组[(2.00±0.85)次/h,(14.45±6.79)％,5.37±1.16]相比均显著增加(F值分别为437.86、14.41、4.29,均P＜0.01);DNFB组、TMA组及NS组均无风团产生.结论 通过抗DNP IgE诱导及DNFB激发后可快速、较稳定地建立小鼠免疫性接触性荨麻疹动物模型.
目的 建立小鼠免疫性接觸性蕁痳疹動物模型.方法 將60隻BALB/c小鼠隨機分5組:抗二硝基苯酚IgE單剋隆抗體(抗DNP IgE)+二硝基氟苯(DNFB)組和抗DNP IgE+偏苯三痠酐(TMA)組小鼠尾靜脈註射抗DNP IgE,24 h後分彆在小鼠耳部塗DNFB和TMA;DNFB組、TMA組、生理氯化鈉液(NS)組小鼠尾靜脈註射NS,24 h後分彆在小鼠雙耳塗DNFB、TMA、NS,觀察小鼠在14d內的風糰、瘙癢情況、肥大細胞脫顆粒數、脾髒指數和皮膚病理.結果 抗DNP IgE+ DNFB組全部(12/12)和抗DNP IgE+ TMA組部分小鼠(8/12)產生風糰;抗DNP IgE+ DNFB組及抗DNP IgE+ TMA組小鼠的搔抓次數[30 min時分彆為(31.58±3.58)次/h,(22.17±3.81)次/h]、肥大細胞脫顆粒率[(70.21±26.01)％,(54.25±39.57)％]及脾髒指數(7.54±1.56,7.87±1.18)與NS組[(2.00±0.85)次/h,(14.45±6.79)％,5.37±1.16]相比均顯著增加(F值分彆為437.86、14.41、4.29,均P＜0.01);DNFB組、TMA組及NS組均無風糰產生.結論 通過抗DNP IgE誘導及DNFB激髮後可快速、較穩定地建立小鼠免疫性接觸性蕁痳疹動物模型.
목적 건립소서면역성접촉성담마진동물모형.방법 장60지BALB/c소서수궤분5조:항이초기분분IgE단극륭항체(항DNP IgE)+이초기불분(DNFB)조화항DNP IgE+편분삼산항(TMA)조소서미정맥주사항DNP IgE,24 h후분별재소서이부도DNFB화TMA;DNFB조、TMA조、생리록화납액(NS)조소서미정맥주사NS,24 h후분별재소서쌍이도DNFB、TMA、NS,관찰소서재14d내적풍단、소양정황、비대세포탈과립수、비장지수화피부병리.결과 항DNP IgE+ DNFB조전부(12/12)화항DNP IgE+ TMA조부분소서(8/12)산생풍단;항DNP IgE+ DNFB조급항DNP IgE+ TMA조소서적소조차수[30 min시분별위(31.58±3.58)차/h,(22.17±3.81)차/h]、비대세포탈과립솔[(70.21±26.01)％,(54.25±39.57)％]급비장지수(7.54±1.56,7.87±1.18)여NS조[(2.00±0.85)차/h,(14.45±6.79)％,5.37±1.16]상비균현저증가(F치분별위437.86、14.41、4.29,균P＜0.01);DNFB조、TMA조급NS조균무풍단산생.결론 통과항DNP IgE유도급DNFB격발후가쾌속、교은정지건립소서면역성접촉성담마진동물모형.
Objective To establish an animal model for immunological contact urticaria in mice.Methods A total of 60 BALB/c mice were randomly and equally divided into 5 groups:anti-dinitrophenol IgE monoclonal antibody (anti-DNP IgE) + 2,4-dinitrofluorobenzene (DNFB) group and anti-DNP IgE + trimellitic anhydride (TMA) group both injected with anti-DNP IgE via tail veins firstly,followed by topical treatment with DNFB and TMA respectively on the ears at 24 hours after the injection,DNFB group,TMA group and normal saline (NS) group all injected with NS via the tail vein firstly,followed by topical treatment with DNFB,TMA and NS on the ears 24 hours after the injection.In the following 14 days,mice were observed daily for the appearance of wheals and for scratching behavior.All the mice were sacrificed at the end of the study followed by determination of the percentage of degranulated mast cells and spleen index as well as observation of pathological changes.Results Wheals were observed in all the mice (12/12) in the anti-DNP IgE + DNFB group,some mice (8/12) in the anti-DNP IgE + TMA group,but not observed in any mice in the other 3 groups.Compared with the NS group,both the anti-DNP IgE + DNFB group and anti-DNP IgE + TMA group showed a significant increase in the percentage of degranulated mast cells (70.21％ ± 26.01％ and 54.25％ ± 39.57％ vs.14.45％ ±6.79％,F=14.41,P=0.000),spleen index (7.54 ± 1.56 and 7.87 ± 1.18 vs.5.37 ± 1.16,F=4.29,P=0.004) and scratching frequency ((31.58 ± 3.58)/h and (22.17 ± 3.81)/h vs.(2.00 ± 0.85)/h at 30 minutes,F =437.86,P ＜ 0.01).Conclusion A stable mouse model for immunological contact urticaria can be established quickly by sensitization with anti-DNP IgE and challenge with DNFB.
