CAJ | 학술논문

目的：探讨尿素加盐酸胍裂解精子的有效性。方法将80例少弱畸精子症患者按随机数字表法分成两组，每组40例，采集精液标本提取精子，分别应用尿素加盐酸胍裂解法或尿素裂解法对精子进行裂解。结果尿素加盐酸胍组得到蛋白质浓度、蛋白质点分别为（1．25±0．13）μg／μl、（864±52）个，均明显高于尿素组的（0．74±0．15）μg／μl、（718±63）个（P＜0．05）。结论尿素加盐酸胍裂解法能得到更丰富、更全面的精子蛋白质种类，并提高蛋白质含量，使双向电泳图谱更加完善，更有利于寻找差异蛋白质。
목적：탐토뇨소가염산고렬해정자적유효성。방법장80례소약기정자증환자안수궤수자표법분성량조，매조40례，채집정액표본제취정자，분별응용뇨소가염산고렬해법혹뇨소렬해법대정자진행렬해。결과뇨소가염산고조득도단백질농도、단백질점분별위（1．25±0．13）μg／μl、（864±52）개，균명현고우뇨소조적（0．74±0．15）μg／μl、（718±63）개（P＜0．05）。결론뇨소가염산고렬해법능득도경봉부、경전면적정자단백질충류，병제고단백질함량，사쌍향전영도보경가완선，경유리우심조차이단백질。
Objective To investigate the effectiveness of urea and guanidine hydrochloride on sperm fragmentation.Methods Eighty patients with oligoasthenoteratozoospermia were randomly divided into two groups according to the random number table, with 40 cases in each group.Patient′s sperms were collected to extract sperm cells.Urea alone and guanidine hydrochloride plus urea pyrolysis were applied to sperm fragmentation,respectively.Results The protein concentration and protein spot in the urea and guanidine hydrochloride group were significantly higher than those in the urea group(( 1.25 ±0.13)μg/μl vs.(0.74 ±0.15)μg/μl,(864 ±52) vs.(718 ±63), P<0.05).Conclusion The fragmentation with urea and guanidine hydrochloride achieves richer and more comprehensive sperm protein species,a higher protein content, and more perfect two-dimensional gel electrophoresis profiles, which might be conducive to finding the differential proteins.