山东体育科技
山東體育科技
산동체육과기
SHANDONG SPORTS SCIENCE & TECHNOLOGY
2015年
3期
87-89
,共3页
耐力运动%氧化应激%心肌组织%SD 大鼠
耐力運動%氧化應激%心肌組織%SD 大鼠
내력운동%양화응격%심기조직%SD 대서
endurance exercise training%oxidative stress%myocardial tissue%SD rats
目的:观察不同强度耐力训练对雄性大鼠的心肌氧化应激水平影响,探讨增强心肌抗氧化能力的适宜训练方式。方法:选用6周龄健康雄性大鼠40只,建立大鼠耐力跑台模型。将选取的大鼠随机分为四组:安静对照组(C 组,10只);低强度组(L 组,10只);中等强度组(M组,10只);高强度组(H 组,10只)。四组进行为期6周的跑台实验后,用生化方法分别检测各组大鼠的氧化应激相关指标包括:心肌组织的超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH -Px)活力以及丙二醛(MDA)的含量。结果:四组不同强度耐力运动训练6周后,结果发现,与安静对照组相比,心肌组织的 SOD 活力在中强度组显著上升,而在高强度组显著下降(P <0.01);GSH -Px 活力在低强度组和中等强度组显著上升,而在高强度组显著下降(P <0.05或 P <0.01);与安静对照组相比,高强度组的心肌组织 MDA 水平显著增高(P <0.01)。结论:低中强度的耐力训练能有效提高心肌的抗氧化能力,尤其是中等强度的耐力训练。
目的:觀察不同彊度耐力訓練對雄性大鼠的心肌氧化應激水平影響,探討增彊心肌抗氧化能力的適宜訓練方式。方法:選用6週齡健康雄性大鼠40隻,建立大鼠耐力跑檯模型。將選取的大鼠隨機分為四組:安靜對照組(C 組,10隻);低彊度組(L 組,10隻);中等彊度組(M組,10隻);高彊度組(H 組,10隻)。四組進行為期6週的跑檯實驗後,用生化方法分彆檢測各組大鼠的氧化應激相關指標包括:心肌組織的超氧化物歧化酶(SOD)活力、穀胱甘肽過氧化物酶(GSH -Px)活力以及丙二醛(MDA)的含量。結果:四組不同彊度耐力運動訓練6週後,結果髮現,與安靜對照組相比,心肌組織的 SOD 活力在中彊度組顯著上升,而在高彊度組顯著下降(P <0.01);GSH -Px 活力在低彊度組和中等彊度組顯著上升,而在高彊度組顯著下降(P <0.05或 P <0.01);與安靜對照組相比,高彊度組的心肌組織 MDA 水平顯著增高(P <0.01)。結論:低中彊度的耐力訓練能有效提高心肌的抗氧化能力,尤其是中等彊度的耐力訓練。
목적:관찰불동강도내력훈련대웅성대서적심기양화응격수평영향,탐토증강심기항양화능력적괄의훈련방식。방법:선용6주령건강웅성대서40지,건립대서내력포태모형。장선취적대서수궤분위사조:안정대조조(C 조,10지);저강도조(L 조,10지);중등강도조(M조,10지);고강도조(H 조,10지)。사조진행위기6주적포태실험후,용생화방법분별검측각조대서적양화응격상관지표포괄:심기조직적초양화물기화매(SOD)활력、곡광감태과양화물매(GSH -Px)활력이급병이철(MDA)적함량。결과:사조불동강도내력운동훈련6주후,결과발현,여안정대조조상비,심기조직적 SOD 활력재중강도조현저상승,이재고강도조현저하강(P <0.01);GSH -Px 활력재저강도조화중등강도조현저상승,이재고강도조현저하강(P <0.05혹 P <0.01);여안정대조조상비,고강도조적심기조직 MDA 수평현저증고(P <0.01)。결론:저중강도적내력훈련능유효제고심기적항양화능력,우기시중등강도적내력훈련。
Objective:To analyze the effects of different types of endurance training on oxidative stress of myocardial tissue in Sprague -Dawley rats,and thereby provide rational movement scheme to improve the myocardial antioxidant capacity.Methods:A total of 40 male SD rats (6 weeks)were enrolled in the study.The study consisted of control group (n =10 ),low intensity exercise group (n =10),moderate intensity exercise group (n =10)and highly intensity exercise group (n =10).The endurance exercise training groups exercised for 6 weeks and the control group maintained their daily eating habits.Suzperoxide dismutase (SOD)activity,glutathione peroxidase (GSH -Px)activity and malondialdehyde (MDA) content were measured after the 6 weeks experiment.Results:After 6 weeks endurance exercise training intervention,compared with control group,the SOD activity were higher in moderate intensity exercise group (P <0.01 ),and lighter in highly intensity exercise group (P <0.01),the GSH -Px activity were higher in low and moderate intensity exercise group(P <0.05 or P <0.01),and lighter in highly intensity exercise group(P <0.01).Moreover,the levels of MDA significantly enhanced in highly intensity exercise group(P <0.01).Conclusion:The low and moderate intensity exercise groups were effective in improving the myocardial antioxidant capacity of rats,particularly in moderate intensity exercise group.