蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
ACTA ACADEMIAE MEDICINAE BENGBU
2015年
6期
712-715
,共4页
李小双%刘丽%罗菲菲%解启莲
李小雙%劉麗%囉菲菲%解啟蓮
리소쌍%류려%라비비%해계련
地塞米松%脓毒症%髓源抑制性细胞%小鼠
地塞米鬆%膿毒癥%髓源抑製性細胞%小鼠
지새미송%농독증%수원억제성세포%소서
dexamethasone%sepsis%myeloid-derived suppressor cell%mice
目的：：观察小剂量地塞米松对脂多糖(LPS)诱导的脓毒症小鼠CD11b+Gr-1+髓源抑制性细胞(MDSCs)的影响。方法：将145只BALB/c雄性小鼠随机分成正常对照组(NC组)35只,脓毒症组(SE组)和小剂量地塞米松干预组(SD组)各55只；SE组和SD组腹腔注射LPS 10 mg/kg制备脓毒症小鼠模型,NC组腹腔注射等量0.9%氯化钠注射液。 SD组于注射LPS 2 h后经尾静脉注入地塞米松0.3 mg/kg,SE组和NC组经尾静脉注入等量0.9%氯化钠注射液。注射LPS后时刻记为0 h,于6、12、24 h检测血清干扰素-γ及白细胞介素-10水平,于12、24、48、72 h检测小鼠脾脏及骨髓中CD11b+Gr-1+ MDSCs的百分比。结果：与NC组比较,SD组及SE组在实验6 h干扰素-γ和12 h白细胞介素-10水平均增加(P<0.05~P<0.01)；SD组与SE组差异亦有统计学意义(P<0.05)。与NC组比较,SE组脾脏MDSCs百分比在12~72 h均明显升高(P<0.01),SD组在12~48 h亦均升高(P<0.01)；而SE组和SD组在24~72 h差异亦均有统计学意义(P<0.01)。与NC组比较,SE组和SD组骨髓MDSCs百分比在48 h和72 h均升高(P<0.05~P<0.01)；而SE组和SD组在48 h和72 h差异亦均有统计学意义(P<0.05和P<0.01)。结论：小剂量地塞米松可抑制LPS诱导的脓毒症小鼠MDSCs的产生及聚集。
目的：：觀察小劑量地塞米鬆對脂多糖(LPS)誘導的膿毒癥小鼠CD11b+Gr-1+髓源抑製性細胞(MDSCs)的影響。方法：將145隻BALB/c雄性小鼠隨機分成正常對照組(NC組)35隻,膿毒癥組(SE組)和小劑量地塞米鬆榦預組(SD組)各55隻；SE組和SD組腹腔註射LPS 10 mg/kg製備膿毒癥小鼠模型,NC組腹腔註射等量0.9%氯化鈉註射液。 SD組于註射LPS 2 h後經尾靜脈註入地塞米鬆0.3 mg/kg,SE組和NC組經尾靜脈註入等量0.9%氯化鈉註射液。註射LPS後時刻記為0 h,于6、12、24 h檢測血清榦擾素-γ及白細胞介素-10水平,于12、24、48、72 h檢測小鼠脾髒及骨髓中CD11b+Gr-1+ MDSCs的百分比。結果：與NC組比較,SD組及SE組在實驗6 h榦擾素-γ和12 h白細胞介素-10水平均增加(P<0.05~P<0.01)；SD組與SE組差異亦有統計學意義(P<0.05)。與NC組比較,SE組脾髒MDSCs百分比在12~72 h均明顯升高(P<0.01),SD組在12~48 h亦均升高(P<0.01)；而SE組和SD組在24~72 h差異亦均有統計學意義(P<0.01)。與NC組比較,SE組和SD組骨髓MDSCs百分比在48 h和72 h均升高(P<0.05~P<0.01)；而SE組和SD組在48 h和72 h差異亦均有統計學意義(P<0.05和P<0.01)。結論：小劑量地塞米鬆可抑製LPS誘導的膿毒癥小鼠MDSCs的產生及聚集。
목적：：관찰소제량지새미송대지다당(LPS)유도적농독증소서CD11b+Gr-1+수원억제성세포(MDSCs)적영향。방법：장145지BALB/c웅성소서수궤분성정상대조조(NC조)35지,농독증조(SE조)화소제량지새미송간예조(SD조)각55지；SE조화SD조복강주사LPS 10 mg/kg제비농독증소서모형,NC조복강주사등량0.9%록화납주사액。 SD조우주사LPS 2 h후경미정맥주입지새미송0.3 mg/kg,SE조화NC조경미정맥주입등량0.9%록화납주사액。주사LPS후시각기위0 h,우6、12、24 h검측혈청간우소-γ급백세포개소-10수평,우12、24、48、72 h검측소서비장급골수중CD11b+Gr-1+ MDSCs적백분비。결과：여NC조비교,SD조급SE조재실험6 h간우소-γ화12 h백세포개소-10수평균증가(P<0.05~P<0.01)；SD조여SE조차이역유통계학의의(P<0.05)。여NC조비교,SE조비장MDSCs백분비재12~72 h균명현승고(P<0.01),SD조재12~48 h역균승고(P<0.01)；이SE조화SD조재24~72 h차이역균유통계학의의(P<0.01)。여NC조비교,SE조화SD조골수MDSCs백분비재48 h화72 h균승고(P<0.05~P<0.01)；이SE조화SD조재48 h화72 h차이역균유통계학의의(P<0.05화P<0.01)。결론：소제량지새미송가억제LPS유도적농독증소서MDSCs적산생급취집。
Objective:To investigate the effects of low dose of dexamethasone on CD11b+ Gr-1 + myeloid-derived suppressor cells ( MDSCs) in septic mice induced by lipopolysaccharide( LPS) . Methods:The septic mice model was established by the intraperitoneal injection of LPS in BALB/c mice. The mice were randomly divided into the normal control group( NC group) ,sepsis group( SE group) and sepsis combined with dexamethasone group(SD group). The levels of serum interferon-γ and interleukin-10 were detected at 6,12 and 24 hours. The percentage of CD11b+ Gr-1 + MDSCs in spleen and bone marrow of mice were detected at 12,24,48 and 72 hours. Results:Compared with the NC group,the levels of interferon-γ at 6 hours and interleukin-10 at 12 hours in SE group and SD group increased significantly(P <0. 05 to P <0. 01),and the difference of which between SE group and SD group was not statistically different( P<0. 05). Compared with the NC group,the percentages of MDSCs in spleen at 12 to 72 hours in SE group and at 12 to 48 hours in SD group increased significantly( P<0. 01),and the differences of the percentage of MDSCs at 24 to 72 hours between SE group and SD group were statistically different( P<0. 05). Compared with the NC group,the percentages of MDSCs in bone marrow of SE and SD group increased significantly at 48 hours and 72 hours( P<0. 05 to P<0. 01). the differences of which at 48 and 72 hours between SE and SD groups were statistically significant(P<0. 05 and P<0. 01). Conclusions:Low dose of dexamethasone can inhibit the production and accumulation of MDSCs in septic mice induced by LPS.
