CAJ | 학술논문

目的：探讨基因芯片技术在结核病临床快速诊断中的应用价值。方法应用基因芯片技术对74例临床标本进行链霉素、乙胺丁醇、异烟肼和利福平耐药检测，以罗氏(L-J)绝对浓度法药敏试验为金标准，对基因芯片技术的检测效果进行评价。结果有39例结果完全符合，符合率为52.7%；基因芯片技术检测链霉素、异烟肼、利福平和乙胺丁醇的结核菌药物敏感试验的敏感性分别为91.2%、57.7%、87.3%和78.1%，特异性均为100%，其中异烟肼和乙胺丁醇结果的差异有统计学意义。结论应用基因芯片技术检测四种抗结核药物敏感性试验，可弥补传统药敏试验方法耗时长的不足，为临床使用链霉素、乙胺丁醇、利福平和异烟肼提供预见性用药建议，可与罗氏金标准方法互为补充检测。
목적：탐토기인심편기술재결핵병림상쾌속진단중적응용개치。방법응용기인심편기술대74례림상표본진행련매소、을알정순、이연정화리복평내약검측，이라씨(L-J)절대농도법약민시험위금표준，대기인심편기술적검측효과진행평개。결과유39례결과완전부합，부합솔위52.7%；기인심편기술검측련매소、이연정、리복평화을알정순적결핵균약물민감시험적민감성분별위91.2%、57.7%、87.3%화78.1%，특이성균위100%，기중이연정화을알정순결과적차이유통계학의의。결론응용기인심편기술검측사충항결핵약물민감성시험，가미보전통약민시험방법모시장적불족，위림상사용련매소、을알정순、리복평화이연정제공예견성용약건의，가여라씨금표준방법호위보충검측。
Objective To explore the application value of gene chip technology in the rapid diagnosis of tuberculosis in clinic. Methods The application of gene chip technology in clinical specimens of 74 cases of streptomycin , ethambutol , isoniazid and ri-fampin resistance detection by L-J absolute concentration method, Roche drug sensitivity test as a gold standard, to evaluate the effect of gene chip detection technology. Results 39 cases results in full compliance, the coincidence rate was 52. 7% ; the sensitivity of gene chip technology to detect streptomycin ,isoniazid, rifampicin and ethambutol tuberculosis drug sensitivity test were respectively 91. 2% , 57. 7% , 87. 3% and 78. 1% , the specificity was 100% , the difference was statistically significant among the isoniazid and ethambutol results. Conclusion The four kinds of anti tuberculosis drug susceptibility test by gene chip technique, which can make up the traditional test method of drug sensitivity of time-consuming shortage, streptomycin, ethambutol, isoniazid and rifampin provide predictability recommendations for clinical use, and L-J goldstandard method of complementary detection. .