蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
ACTA ACADEMIAE MEDICINAE BENGBU
2015年
6期
708-711
,共4页
陈臻瑶%巢柳荫%仲明星%薄秀梅
陳臻瑤%巢柳蔭%仲明星%薄秀梅
진진요%소류음%중명성%박수매
动脉粥样硬化%普罗布考%塞来昔布%大鼠
動脈粥樣硬化%普囉佈攷%塞來昔佈%大鼠
동맥죽양경화%보라포고%새래석포%대서
atherosclerosis%probucol%celecoxib%rat
目的::探讨普罗布考与塞来昔布联合应用对大鼠动脉粥样硬化的影响。方法:SD雄性大鼠40只,随机分为正常组、模型组、普罗布考组、塞来昔布组及普罗布考与塞来昔布联合组(联合组),每组各8只。正常组给予基础饲料喂养,模型组为建立动脉粥样硬化模型,予以高脂饲料+维生素D3+动脉内膜球囊损伤术,普罗布考组、塞来昔布组、联合组同法造模后分别予以普罗布考、塞来昔布、普罗布考联合塞来昔布灌胃。药物干预12周后,检测血清总胆固醇( TC)、三酰甘油( TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C),酶联免疫吸附试验法检测血清C反应蛋白(CRP)和正五聚体蛋白3(PTX3),HE染色观察胸主动脉的病理形态改变并计算动脉内膜、中膜厚度及内膜/中膜厚度比。结果:与正常组比较,模型组、普罗布考组、塞来昔布组和联合组的TC、LDL-C、CRP均明显升高(P<0.01);与模型组比较,普罗布考组和联合组的TC、LDL-C、CRP、PTX3均显著降低(P<0.01);与正常组比较,普罗布考组、塞来昔布组和联合组的内膜厚度、中膜厚度、内膜/中膜厚度比均明显增加(P<0.01),普罗布考组、塞来昔布组和联合组与模型组比较,内膜厚度、内膜/中膜厚度比均显著减小,中膜厚度增加(P<0.01)。结论:普罗布考和塞来昔布联合应用可明显降低动脉粥样硬化大鼠血脂和炎性因子水平,有一定的抗动脉粥样硬化作用。
目的::探討普囉佈攷與塞來昔佈聯閤應用對大鼠動脈粥樣硬化的影響。方法:SD雄性大鼠40隻,隨機分為正常組、模型組、普囉佈攷組、塞來昔佈組及普囉佈攷與塞來昔佈聯閤組(聯閤組),每組各8隻。正常組給予基礎飼料餵養,模型組為建立動脈粥樣硬化模型,予以高脂飼料+維生素D3+動脈內膜毬囊損傷術,普囉佈攷組、塞來昔佈組、聯閤組同法造模後分彆予以普囉佈攷、塞來昔佈、普囉佈攷聯閤塞來昔佈灌胃。藥物榦預12週後,檢測血清總膽固醇( TC)、三酰甘油( TG)、低密度脂蛋白膽固醇(LDL-C)、高密度脂蛋白膽固醇(HDL-C),酶聯免疫吸附試驗法檢測血清C反應蛋白(CRP)和正五聚體蛋白3(PTX3),HE染色觀察胸主動脈的病理形態改變併計算動脈內膜、中膜厚度及內膜/中膜厚度比。結果:與正常組比較,模型組、普囉佈攷組、塞來昔佈組和聯閤組的TC、LDL-C、CRP均明顯升高(P<0.01);與模型組比較,普囉佈攷組和聯閤組的TC、LDL-C、CRP、PTX3均顯著降低(P<0.01);與正常組比較,普囉佈攷組、塞來昔佈組和聯閤組的內膜厚度、中膜厚度、內膜/中膜厚度比均明顯增加(P<0.01),普囉佈攷組、塞來昔佈組和聯閤組與模型組比較,內膜厚度、內膜/中膜厚度比均顯著減小,中膜厚度增加(P<0.01)。結論:普囉佈攷和塞來昔佈聯閤應用可明顯降低動脈粥樣硬化大鼠血脂和炎性因子水平,有一定的抗動脈粥樣硬化作用。
목적::탐토보라포고여새래석포연합응용대대서동맥죽양경화적영향。방법:SD웅성대서40지,수궤분위정상조、모형조、보라포고조、새래석포조급보라포고여새래석포연합조(연합조),매조각8지。정상조급여기출사료위양,모형조위건립동맥죽양경화모형,여이고지사료+유생소D3+동맥내막구낭손상술,보라포고조、새래석포조、연합조동법조모후분별여이보라포고、새래석포、보라포고연합새래석포관위。약물간예12주후,검측혈청총담고순( TC)、삼선감유( TG)、저밀도지단백담고순(LDL-C)、고밀도지단백담고순(HDL-C),매련면역흡부시험법검측혈청C반응단백(CRP)화정오취체단백3(PTX3),HE염색관찰흉주동맥적병리형태개변병계산동맥내막、중막후도급내막/중막후도비。결과:여정상조비교,모형조、보라포고조、새래석포조화연합조적TC、LDL-C、CRP균명현승고(P<0.01);여모형조비교,보라포고조화연합조적TC、LDL-C、CRP、PTX3균현저강저(P<0.01);여정상조비교,보라포고조、새래석포조화연합조적내막후도、중막후도、내막/중막후도비균명현증가(P<0.01),보라포고조、새래석포조화연합조여모형조비교,내막후도、내막/중막후도비균현저감소,중막후도증가(P<0.01)。결론:보라포고화새래석포연합응용가명현강저동맥죽양경화대서혈지화염성인자수평,유일정적항동맥죽양경화작용。
Objective:To investigate the effects of probucol combined with celecoxib on atherosclerosis in rats. Methods:Forty male SD rats were randomly divided into the normal group, model group, probucol group, celecoxib group and probucol combined with celecoxib group(8 rats each group). Normal group was fed with standard forage. Rat model with atherosclerosis was established,and treated with high lipid,Vitamin D3 plus balloon injury of endarterium. The probucol group,celecoxib group and probucol combined with celecoxib group were treated with oral gavage,respectively. After 12 weeks of drug intervention in all groups,the serum levels of total cholesterol( TC) ,triglyceride( TG) ,low density lipoprotein-cholesterol( LDL-C) and high density lipoprotein-cholesterol( HDL-C) were detected,the levels of serum C-reactive protein(CRP) and Pentraxin3(PTX3) were determined by ELISA,and the pathomorphological changes of thoracic aorta were measured using HE staining and the thickness of arterial intima and media and thickness ratio of intima to media were calculated. Results:Compared with the normal group,the serum levels of TC,LDL-C and CRP in model group,probucol group,celecoxib group and probucol combined with celecoxib group significantly increased(P<0. 01). Compared with the model group, the serum levels of TC,LDL-C,CRP and PTX3 in probucol group and probucol combined with celecoxib group significantly decreased (P<0. 01). Compared with the normal group,the thickness of arterial intima and media,and thickness ratio of intima to media in probucol group,celecoxib group and probucol combined with celecoxib group significantly increased(P<0. 01). Compared with the model group,the thickness of arterial intima and thickness ratio of intima to media significantly decreased and the thickness of arterial media significantly increased in probucol group,celecoxib group and probucol combined with celecoxib group(P<0. 01). Conclusions:The combination use of probucol and celecoxib can significantly decreased the levels of serum lipids and inflammatory factors in rats with atherosclerosis,which has certain effects on anti-atherosclerosis.