烟草科技
煙草科技
연초과기
TOBACCO SCIENCE & TECHNOLOGY
2015年
6期
1-8
,共8页
李泽锋%魏攀%夏玉珍%王中%武明珠%王燃%罗朝鹏%金立锋%杨军%林福呈%李锋
李澤鋒%魏攀%夏玉珍%王中%武明珠%王燃%囉朝鵬%金立鋒%楊軍%林福呈%李鋒
리택봉%위반%하옥진%왕중%무명주%왕연%라조붕%금립봉%양군%림복정%리봉
普通烟草%林烟草%绒毛状烟草%牻牛儿基牻牛儿基焦磷酸合成酶(GGPPS)%基因家族%全基因组
普通煙草%林煙草%絨毛狀煙草%牻牛兒基牻牛兒基焦燐痠閤成酶(GGPPS)%基因傢族%全基因組
보통연초%림연초%융모상연초%방우인기방우인기초린산합성매(GGPPS)%기인가족%전기인조
Nicotiana tabacum%Nicotiana sylvestris%Nicotiana tomentosiformis%Geranylgeranyl pyrophosphate synthase(GGPPS)%Gene family%Whole genome
为了揭示烟草牻牛儿基牻牛儿基焦磷酸合成酶（Geranylgeranyl pyrophosphate synthase， GGPPS）基因家族的特征和功能，利用生物信息学方法对烟草GGPPS家族进行了全基因组查找、鉴定及相关分析。在普通烟草中共鉴定出9个成员（大亚基7个、小亚基2个），林烟草、绒毛状烟草中分别鉴定出5个成员（大亚基4个、小亚基1个）。GGPPS的开放阅读框长度为999~1119 bp，编码332~372个氨基酸，蛋白质分子量为36.2~40.7 kD，等电点为5.41~8.32。大亚基和小亚基基因分别含有1个和2个外显子；大亚基与小亚基均具有DD（XX）1-2D和CXXXC保守性基序，但存在一定的差异。在进化过程中，烟草GGPPS家族可能发生了串联重复复制和片断复制；普通烟草GGPPS家族可能发生了基因丢失，NtabGGPPS1-1和NtabGGPPS1-2较其他成员进化速率快，同时可能受到了净化选择。
為瞭揭示煙草牻牛兒基牻牛兒基焦燐痠閤成酶（Geranylgeranyl pyrophosphate synthase， GGPPS）基因傢族的特徵和功能，利用生物信息學方法對煙草GGPPS傢族進行瞭全基因組查找、鑒定及相關分析。在普通煙草中共鑒定齣9箇成員（大亞基7箇、小亞基2箇），林煙草、絨毛狀煙草中分彆鑒定齣5箇成員（大亞基4箇、小亞基1箇）。GGPPS的開放閱讀框長度為999~1119 bp，編碼332~372箇氨基痠，蛋白質分子量為36.2~40.7 kD，等電點為5.41~8.32。大亞基和小亞基基因分彆含有1箇和2箇外顯子；大亞基與小亞基均具有DD（XX）1-2D和CXXXC保守性基序，但存在一定的差異。在進化過程中，煙草GGPPS傢族可能髮生瞭串聯重複複製和片斷複製；普通煙草GGPPS傢族可能髮生瞭基因丟失，NtabGGPPS1-1和NtabGGPPS1-2較其他成員進化速率快，同時可能受到瞭淨化選擇。
위료게시연초방우인기방우인기초린산합성매（Geranylgeranyl pyrophosphate synthase， GGPPS）기인가족적특정화공능，이용생물신식학방법대연초GGPPS가족진행료전기인조사조、감정급상관분석。재보통연초중공감정출9개성원（대아기7개、소아기2개），림연초、융모상연초중분별감정출5개성원（대아기4개、소아기1개）。GGPPS적개방열독광장도위999~1119 bp，편마332~372개안기산，단백질분자량위36.2~40.7 kD，등전점위5.41~8.32。대아기화소아기기인분별함유1개화2개외현자；대아기여소아기균구유DD（XX）1-2D화CXXXC보수성기서，단존재일정적차이。재진화과정중，연초GGPPS가족가능발생료천련중복복제화편단복제；보통연초GGPPS가족가능발생료기인주실，NtabGGPPS1-1화NtabGGPPS1-2교기타성원진화속솔쾌，동시가능수도료정화선택。
In order to reveal the characteristics and functions of GGPPS (Geranylgeranyl pyrophosphate synthase)gene family in tobacco, the GGPPS family in Nicotiana tabacum(N. tabacum), Nicotiana sylvestris (N. sylvestris) and Nicotiana tomentosiformis(N. tomentosiformis) were searched, identified and analyzed in whole genome level through in silico methods. Nine members (seven large subunits and two small subunits) in N. tabacum, and five ones (four large subunits and one small subunit) in N. sylvestris and N. tomentosiformis were identified respectively. The open reading frames of GGPPS were 999-1 119 bp in <br> length, coded 333-372 amino acids with protein molecular weights of 36.3-40.7 kDa and pI in the range of 5.41-8.32. Small subunit genes consisted of two exons, while there was only one exon in large subunit genes. Both genes comprised DD(XX)1-2D and CXXXC conservative motifs, though certain differences existed. In the course of evolution, GGPPS family in tobacco might have undergone both tandem duplication and segmental duplication, and gene loss event probably occurred in N. tabacum. In addition, NtabGGPPS1-1 and NtabGGPPS1-2 evolved faster than the other ones and might be under purifying selection.
