CAJ | 학술논문

目的研究孕鼠产前慢性应激(PS)对子代大鼠学习记忆的影响及其可能的分子机制.方法通过SD孕鼠妊娠第14-21天每天进行3次束缚,每次45 min,建立PS模型,仔鼠为研究对象标记为PS组,无束缚孕鼠的仔鼠为对照组,每组又分为雌雄组,各20只.利用Morris水迷宫分析系统检测学习记忆能力,放射免疫法测定血清基础皮质酮(COR)和促皮质素(ACTH)水平,通过高尔基染色法观察海马CA1区神经元树突棘的形态和密度变化,并进行统计学分析.结果在Morris水迷宫测试中,雌性PS组的逃逸潜伏期(EL)明显长于雌性对照组,差异具有统计学意义(F =4.533,P＜0.05);第5天的EL差异具有统计学意义(=2.788,P＜0.01).雄性PS组的EL明显长于雄性对照组,差异具有统计学意义(F=6.101,P＜0.05);第2天EL差异具有统计学意义(t =3.051,P＜0.01).在空间探索实验中,PS组的目标象限停留时间与同性别对照组相似,差异无统计学意义(P＞0.05).PS组子代雌性大鼠的血清基础COR水平较同性别对照组升高,差异具有统计学意义(t =3.658,P＜0.01);PS组子代雄性大鼠的血清基础ACTH水平较同性别对照组升高,差异具有统计学意义(t=2.319,P ＜0.05).高尔基染色显示PS组仔鼠海马CA1区锥体神经元树突形态简单化,表现为树突分支减少,树突棘密度减少,与对照组比较差异具有统计学意义(t=-3.072,P＜0.01).结论孕鼠产前慢性束缚应激引起仔鼠下丘脑-垂体-肾上腺轴功能紊乱,损害仔鼠空间学习记忆能力,可能与仔鼠海马CA1区神经元树突棘的形态变化和密度降低有关.
목적 연구잉서산전만성응격(PS)대자대대서학습기억적영향급기가능적분자궤제.방법 통과SD잉서임신제14-21천매천진행3차속박,매차45 min,건립PS모형,자서위연구대상표기위PS조,무속박잉서적자서위대조조,매조우분위자웅조,각20지.이용Morris수미궁분석계통검측학습기억능력,방사면역법측정혈청기출피질동(COR)화촉피질소(ACTH)수평,통과고이기염색법관찰해마CA1구신경원수돌극적형태화밀도변화,병진행통계학분석.결과 재Morris수미궁측시중,자성PS조적도일잠복기(EL)명현장우자성대조조,차이구유통계학의의(F =4.533,P＜0.05);제5천적EL차이구유통계학의의(=2.788,P＜0.01).웅성PS조적EL명현장우웅성대조조,차이구유통계학의의(F=6.101,P＜0.05);제2천EL차이구유통계학의의(t =3.051,P＜0.01).재공간탐색실험중,PS조적목표상한정류시간여동성별대조조상사,차이무통계학의의(P＞0.05).PS조자대자성대서적혈청기출COR수평교동성별대조조승고,차이구유통계학의의(t =3.658,P＜0.01);PS조자대웅성대서적혈청기출ACTH수평교동성별대조조승고,차이구유통계학의의(t=2.319,P ＜0.05).고이기염색현시PS조자서해마CA1구추체신경원수돌형태간단화,표현위수돌분지감소,수돌극밀도감소,여대조조비교차이구유통계학의의(t=-3.072,P＜0.01).결론 잉서산전만성속박응격인기자서하구뇌-수체-신상선축공능문란,손해자서공간학습기억능력,가능여자서해마CA1구신경원수돌극적형태변화화밀도강저유관.
Objective To study the influence of pregnant rats' prenatal chronic stress (PS) on learning and memory of their offspring rats and its possible molecular mechanisms.Methods Pregnant females were individually restrained for 45 min 3 times a day during pregnancy from day 14 to day 21.Control pregnant females were left undisturbed in their home cages.The rat offsprings were randomly assigned to PS group or control group.Males and females were kept for the study separately.The learning and memory of the developing rat offspring in the Morris water maze were examined.The basal levels of corticosterone (COR) and adreno-cortico-tropic-hormone (ACTH) were analyzed by using radioimmunoassay.The Golgi-Cox impregnation technique was used to compare density and morphology of the CA1 hippocampal dendritic spines.Results The escape latency (EL) to find the platform in the control group was significantly less than that in the PS group in female rat offspring (F =4.533,P ＜ 0.05),and the difference was statistically significant on the 5th day (t =2.788,P ＜ 0.01).EL to find the platform in the control group was significantly less than that in the PS group in male rat offspring (F =6.101,P ＜0.05),and the difference was statistically significant on the second day (t =3.051,P ＜ 0.01).In the space exploration experiments of the water maze,the retention time observed for the control group and the PS group in the goal quadrant was similar(P ＞ 0.05).The basal levels of the serum COR in the PS group were higher than those in the control group of female rat offspring(t =3.658,P ＜ 0.01) and the basal levels of the serum ACTH in the PS group were higher than those in the control group of male rat offsprings(t =2.319,P ＜ 0.05).A simplified pattern was observed in the CA1 hippocampal dendritic spines in the PS group,showing a less extent of dendritic arborization and the density was significantly lower than that in the control group(t =-3.072,P ＜ 0.01).Conclusions Altered function of the hypothalamic-pituitary-adrenal axis in the offspring mediates the cognitive alterations observed following prenatal stress should to be associated with the lower density and simplified pattern of CA1 dendritic spines.