温州医科大学学报
溫州醫科大學學報
온주의과대학학보
Journal of Wenzhou Medical University
2015年
6期
437-439,442
,共4页
李燕明%佟训靓%许宏涛%田埂%郎继东%肖飞%蔡虻
李燕明%佟訓靚%許宏濤%田埂%郎繼東%肖飛%蔡虻
리연명%동훈정%허굉도%전경%랑계동%초비%채맹
高通量测序%医院感染%曲霉%隐球菌%念珠菌
高通量測序%醫院感染%麯黴%隱毬菌%唸珠菌
고통량측서%의원감염%곡매%은구균%념주균
high-throughput sequence%hospital infection%aspergillus%cryptococcus%candida
目的：应用Illumina高通量测序技术对医院内空气环境进行检测，了解医院内不同科室空气中常见微生物的分布特点。方法：利用恒流负压收集器采集北京医院门诊、急诊、外科监护室、呼吸监护室4个科室的空气样本，提取微生物DNA样本，利用Illumina高通量测序技术分析不同科室空气环境中微生物的种类和相对含量。结果：高通量测序的方法可一次性检出上千种微生物，如真菌、细菌、病毒，其优势在于对真菌的鉴定。在外科监护室、呼吸监护室，致病性真菌占空气中真菌的比例较高，分别为60.37%、81.59%，而在门诊、急诊，该比例稍低，分别为53.25%、58.98%。在真菌菌属分类中，霉菌中的曲霉，酵母菌中的隐球菌和念珠菌所占比例较高。结论：高通量测序技术应用于医院环境监测，可提供更多机会性感染的病原菌信息，对改进医院环境监控的技术方法及提高医院感染的监控水平提供实验基础。
目的：應用Illumina高通量測序技術對醫院內空氣環境進行檢測，瞭解醫院內不同科室空氣中常見微生物的分佈特點。方法：利用恆流負壓收集器採集北京醫院門診、急診、外科鑑護室、呼吸鑑護室4箇科室的空氣樣本，提取微生物DNA樣本，利用Illumina高通量測序技術分析不同科室空氣環境中微生物的種類和相對含量。結果：高通量測序的方法可一次性檢齣上韆種微生物，如真菌、細菌、病毒，其優勢在于對真菌的鑒定。在外科鑑護室、呼吸鑑護室，緻病性真菌佔空氣中真菌的比例較高，分彆為60.37%、81.59%，而在門診、急診，該比例稍低，分彆為53.25%、58.98%。在真菌菌屬分類中，黴菌中的麯黴，酵母菌中的隱毬菌和唸珠菌所佔比例較高。結論：高通量測序技術應用于醫院環境鑑測，可提供更多機會性感染的病原菌信息，對改進醫院環境鑑控的技術方法及提高醫院感染的鑑控水平提供實驗基礎。
목적：응용Illumina고통량측서기술대의원내공기배경진행검측，료해의원내불동과실공기중상견미생물적분포특점。방법：이용항류부압수집기채집북경의원문진、급진、외과감호실、호흡감호실4개과실적공기양본，제취미생물DNA양본，이용Illumina고통량측서기술분석불동과실공기배경중미생물적충류화상대함량。결과：고통량측서적방법가일차성검출상천충미생물，여진균、세균、병독，기우세재우대진균적감정。재외과감호실、호흡감호실，치병성진균점공기중진균적비례교고，분별위60.37%、81.59%，이재문진、급진，해비례초저，분별위53.25%、58.98%。재진균균속분류중，매균중적곡매，효모균중적은구균화념주균소점비례교고。결론：고통량측서기술응용우의원배경감측，가제공경다궤회성감염적병원균신식，대개진의원배경감공적기술방법급제고의원감염적감공수평제공실험기출。
Objective: To monitor microorganisms in air borne environment in hospital and to analysis the distribution characters of microorganisms in different departments in hospital.Methods: Air samplers were utilized in four typical departments of hospital for airborne microorganisms’ collection. Microorganisms were identiifed by DNA sequencing analysis by Illumina MiSeq and HiSeq 2000 sequencing systems.Results: The airborne microorganisms from four departments were analyzed for microbiome sequencing and classified as fungi,species, bacteria species and viruses species. The ratio of pathogenic fungi in fungi was different in four departments and the ratio in two intensive care units were higher than its in outpatient department and emergency department. At the species level of fungi, ratio of yeast, mycotic fungus and dimorphic fungi in different depart-ment was shown. The relative ratio of aspergillus in mycotic fungus and the relative ratio of cryptococcus and candida in yeast was shown,respectively.Conclusion: Novel method was utilized in monitoring the air borne microorganisms in hospital environment and the relative ratio of common opportunistic pathogenwas satisifed. It is provided experiment based information in improving the methodology in monitoring hospital infection.
