CAJ | 학술논문

目的：探讨胃癌患者血清胃蛋白酶原（ PG）和抗幽门螺杆菌抗体（ HpAb）联合检测作为胃癌标志物用于临床普查的可行性。方法根据纤维胃镜和病理组织细胞学诊断入选的病例分为三组：正常对照组50例，胃癌组114例，慢性萎缩性胃炎（ CAG）组203例，采用双抗体夹心酶联免疫吸附的方法检测胃蛋白酶原Ⅰ（ PGⅠ）和胃蛋白酶原Ⅱ（ PGⅡ）的水平，计算出PGR（ PGⅠ／PGⅡ），采用尿素酶抗体胶体金法定性检测抗幽门螺杆菌抗体。结果胃癌组PGⅠ与PGR水平明显低于正常对照组，两组比较差异具有统计学意义（P＜0.01）；PGⅡ的含量变化不大，两组比较差异无统计学意义（P＞0.05）；CAG组PGⅠ与PGR水平明显低于正常对照组，两组比较差异具有统计学意义（ P＜0.01）；PGⅡ的含量变化不大，两组比较差异无统计学意义（ P＞0.05）；胃癌组与CAG组PGⅠ、PGR比较差异无统计学意义（ P＞0.05）胃癌组与CAG组的抗HP抗体的阳性率明显高于正常对照组，差异有统计学意义（ P＜0.01）；而胃癌组与CAG组比较差异无统计学意义（ P＞0.05）。结论血清PG和抗幽门螺杆菌抗体（ HpAb）两者联合检测同时阴性可以85％排除胃癌的诊断，作为大规模人群中早期胃癌筛查的有效指标是可行的。
목적：탐토위암환자혈청위단백매원（ PG）화항유문라간균항체（ HpAb）연합검측작위위암표지물용우림상보사적가행성。방법근거섬유위경화병리조직세포학진단입선적병례분위삼조：정상대조조50례，위암조114례，만성위축성위염（ CAG）조203례，채용쌍항체협심매련면역흡부적방법검측위단백매원Ⅰ（ PGⅠ）화위단백매원Ⅱ（ PGⅡ）적수평，계산출PGR（ PGⅠ／PGⅡ），채용뇨소매항체효체금법정성검측항유문라간균항체。결과위암조PGⅠ여PGR수평명현저우정상대조조，량조비교차이구유통계학의의（P＜0.01）；PGⅡ적함량변화불대，량조비교차이무통계학의의（P＞0.05）；CAG조PGⅠ여PGR수평명현저우정상대조조，량조비교차이구유통계학의의（ P＜0.01）；PGⅡ적함량변화불대，량조비교차이무통계학의의（ P＞0.05）；위암조여CAG조PGⅠ、PGR비교차이무통계학의의（ P＞0.05）위암조여CAG조적항HP항체적양성솔명현고우정상대조조，차이유통계학의의（ P＜0.01）；이위암조여CAG조비교차이무통계학의의（ P＞0.05）。결론혈청PG화항유문라간균항체（ HpAb）량자연합검측동시음성가이85％배제위암적진단，작위대규모인군중조기위암사사적유효지표시가행적。
Objectives To investigate clinical survey feasibility of the gastric cancer patients serum pepsinogen ( PG) and Heli-cobacter pylori antibody ( HpAb) as a marker for the detection of gastric cancer.Methods Enrolled patients were divided into three groups:normal control group of 50 cases, gastric cancer 114 cases, chronic atrophic gastritis ( CAG) group of 203 cases, ac-cording to the fibre gastroscopy and pathological cytology diagnosis.Using double antibody sandwich enzyme linked immunosorbent assay for detection of pepsinogenⅠ( PGⅠ) and pepsinogenⅡ( PGⅡ) level, calculate the PGR ( PGⅠ /PGⅡ) , using urease antibody colloidal gold method to qualitatively detect antibodies against Helicobacter pylori.Results The gastric cancer group’ s PG I and PGR levels were significantly lower than the normal control group, with significant differences between the two groups( P<0.01);the content change of PGⅡis little, no significant differences between the two groups (P>0.05);CAG group’s PG Ⅰand PGR levels were significantly lower than the normal control group, with significant difference between the two group ( P <0.01);the PG II content changes were little, no significant differences between the two groups (P>0.05);PGR and PGⅠhad no statistical significance in gastric cancer group and CAG group( P>0.05).Anti HP antibody positive rate in gastric cancer group and the CAG group was significantly higher than that in the normal control group, the difference was statistically significant (P<0.01);but it had no significant differences in gastric cancer group and CAG group ( P>0.05).Conclusions If combined detection of the serum PG and anti Helicobacter pylori antibody ( HpAb) are both negative, 85%gastric cancer can be discharged.it is feasible, as an effective indicator for early gastric cancer screening for large population.