CAJ | 학술논문

对检测白酒中微量甜味剂（安赛蜜、糖精钠、甜蜜素、阿斯巴甜），建立了一种利用超高效液相色谱-三重四级杆串联质谱仪（ESI离子源）的检测方法。样品经酒精前处理后，以BEH C18色谱柱为分离柱，0.1%（v/v）乙酸-1 mmol/L乙酸铵溶液∶乙腈为流动相进行梯度洗脱，经超高效液相色谱（UPLC）分离，采用电喷雾串联四级杆质谱进行多反应监测模式（MRM）进行检测。4种甜味剂线性范围在50～250 ng/mL内，拟合所得线性相关系数均大于0.997。在3个加标水平下，得到样品平均回收率为97.1%～111.3%，相对标准偏差（RSD）均控制在5%以内。该方法简单快捷、准确可靠，可用于白酒中4种甜味剂的同时检测。
대검측백주중미량첨미제（안새밀、당정납、첨밀소、아사파첨），건립료일충이용초고효액상색보-삼중사급간천련질보의（ESI리자원）적검측방법。양품경주정전처리후，이BEH C18색보주위분리주，0.1%（v/v）을산-1 mmol/L을산안용액∶을정위류동상진행제도세탈，경초고효액상색보（UPLC）분리，채용전분무천련사급간질보진행다반응감측모식（MRM）진행검측。4충첨미제선성범위재50～250 ng/mL내，의합소득선성상관계수균대우0.997。재3개가표수평하，득도양품평균회수솔위97.1%～111.3%，상대표준편차（RSD）균공제재5%이내。해방법간단쾌첩、준학가고，가용우백주중4충첨미제적동시검측。
In order to detect the trace sweeteners in Baijiu(liquor) (acesulfame potassium, soluble saccharin, sodium cyclamate and aspartame), a method using UPLC-MS/MS had been developed in this study. Liquor samples were pre-treated by alcohol, then BEH C18 chromatographic column was used as separation column, and 0.1 % (v/v) acetic acid-1mmol/L ammonium acetate solution/acetonitrile were used as mobile phase for gradient elution. After UPLC separation, MS/MS was adopted for multiple reaction monitoring mode (MRM). The experimental re-sults suggested that the linear range of standard curves for those 4 sweeteners was from 50 to 250 ng/mL, and the linear relationship was great-er than 0.997. The spiked recoveries were within 97.1%~111.3%in three levels of standard addition among linear range of standard curves. And RSD was controlled below 5%. Such method was simple, quick, accurate and reliable, and it could be used for simultaneous detection of the 4 sweeteners in Baijiu(liquor).