中华眼视光学与视觉科学杂志
中華眼視光學與視覺科學雜誌
중화안시광학여시각과학잡지
CHINESE JOURNAL OF OPTOMETRY OPHTHALMOLOGY AND VISUAL SCIENCE
2015年
6期
331-334
,共4页
辐射,电离%巩膜成纤维细胞%基质金属蛋白酶2%胶原Ⅰ型
輻射,電離%鞏膜成纖維細胞%基質金屬蛋白酶2%膠原Ⅰ型
복사,전리%공막성섬유세포%기질금속단백매2%효원Ⅰ형
Radiation,ionizing%Human fetal sclera fibroblast%Matrix metalloproteinase 2%Collagen type Ⅰ
目的 研究极低频电磁场(ELF-EMF)作用下人胚胎眼巩膜成纤维细胞(HFSF)中基质金属蛋白酶-2(MMP-2)活性与蛋白表达的变化以及ELF-EMF对Ⅰ型胶原(Collagen Ⅰ)合成的影响.方法 实验研究.根据是否暴露于50 Hz、0.2 mT电磁场或加入MMP-2特异性抑制剂(sc-204092),将实验细胞分为对照组、辐照组和抑制剂组.明胶酶谱法检测各组HFSF中MMP-2酶活性的变化;Western Blot法检测各组HFSF中MMP-2、Collagen Ⅰ蛋白水平的变化.采用单因素方差分析进行数据分析.结果 3组HFSF细胞中MMP-2酶活性、MMP-2、Collagen Ⅰ蛋白表达比较差异均具有统计学意义(F=14.96、139.88、63.46,P均＜0.01);辐照组较对照组MMP-2酶活性和蛋白表达升高(P＜0.05),Collagen Ⅰ蛋白表达下降(P＜0.01);抑制剂组较辐照组MMP-2酶活性和蛋白表达下降(P＜0.01),Collagen Ⅰ蛋白表达升高(P＜0.05).结论 ELF-EMF可能通过影响体外培养的HFSF中MMP-2的酶活性和蛋白表达来调节Collagen Ⅰ的合成.
目的 研究極低頻電磁場(ELF-EMF)作用下人胚胎眼鞏膜成纖維細胞(HFSF)中基質金屬蛋白酶-2(MMP-2)活性與蛋白錶達的變化以及ELF-EMF對Ⅰ型膠原(Collagen Ⅰ)閤成的影響.方法 實驗研究.根據是否暴露于50 Hz、0.2 mT電磁場或加入MMP-2特異性抑製劑(sc-204092),將實驗細胞分為對照組、輻照組和抑製劑組.明膠酶譜法檢測各組HFSF中MMP-2酶活性的變化;Western Blot法檢測各組HFSF中MMP-2、Collagen Ⅰ蛋白水平的變化.採用單因素方差分析進行數據分析.結果 3組HFSF細胞中MMP-2酶活性、MMP-2、Collagen Ⅰ蛋白錶達比較差異均具有統計學意義(F=14.96、139.88、63.46,P均＜0.01);輻照組較對照組MMP-2酶活性和蛋白錶達升高(P＜0.05),Collagen Ⅰ蛋白錶達下降(P＜0.01);抑製劑組較輻照組MMP-2酶活性和蛋白錶達下降(P＜0.01),Collagen Ⅰ蛋白錶達升高(P＜0.05).結論 ELF-EMF可能通過影響體外培養的HFSF中MMP-2的酶活性和蛋白錶達來調節Collagen Ⅰ的閤成.
목적 연구겁저빈전자장(ELF-EMF)작용하인배태안공막성섬유세포(HFSF)중기질금속단백매-2(MMP-2)활성여단백표체적변화이급ELF-EMF대Ⅰ형효원(Collagen Ⅰ)합성적영향.방법 실험연구.근거시부폭로우50 Hz、0.2 mT전자장혹가입MMP-2특이성억제제(sc-204092),장실험세포분위대조조、복조조화억제제조.명효매보법검측각조HFSF중MMP-2매활성적변화;Western Blot법검측각조HFSF중MMP-2、Collagen Ⅰ단백수평적변화.채용단인소방차분석진행수거분석.결과 3조HFSF세포중MMP-2매활성、MMP-2、Collagen Ⅰ단백표체비교차이균구유통계학의의(F=14.96、139.88、63.46,P균＜0.01);복조조교대조조MMP-2매활성화단백표체승고(P＜0.05),Collagen Ⅰ단백표체하강(P＜0.01);억제제조교복조조MMP-2매활성화단백표체하강(P＜0.01),Collagen Ⅰ단백표체승고(P＜0.05).결론 ELF-EMF가능통과영향체외배양적HFSF중MMP-2적매활성화단백표체래조절Collagen Ⅰ적합성.
Objective To observe the matrix metalloproteinase 2 (MMP-2) activity and protein expression in human fetal scleral fibroblasts (HFSFs) exposed to an extremely low frequency electromagnetic field (ELF-EMF) and its impact on collagen Ⅰ synthesis.Methods This was an experimental study.HFSFs were cultured and divided into a control group,radiation group and inhibitor group according to their exposure to 50 Hz,0.2 mT electromagnetic field or joint MMP-2 specific inhibitors (sc-204092).MMP-2 activity was measured by gelatin enzymography.MMP-2 and collagen Ⅰ protein expression were detected by Western Blot.A single factor analysis of variance was used to determine whether there was a difference among these groups.Results There were significant differences between the three groups (F=14.96,139.88,63.46,P＜0.01).Compared to the control group,MMP-2 activity and protein expression increased in the radiation group (P＜0.05) and collagen Ⅰ protein expression decreased (P＜0.01).Compared to the radiation group,MMP-2 activity and protein expression decreased in the inhibitor group (P＜0.01) and collagen Ⅰ protein expression increased (P＜0.05).Conclusion ELF-EMF may regulate the synthesis of collagen Ⅰ by affecting the activity and protein expression of MMP-2.
