武汉轻工大学学报
武漢輕工大學學報
무한경공대학학보
Journal of Wuhan Polytechnic University
2015年
2期
60-64
,共5页
菱角壳%气相色谱-质谱法( GC-MS)%邻苯二甲酸二丁酯( DBP)
蔆角殼%氣相色譜-質譜法( GC-MS)%鄰苯二甲痠二丁酯( DBP)
릉각각%기상색보-질보법( GC-MS)%린분이갑산이정지( DBP)
Trapa bispinosa Roxb%Gas chromatography-mass spectrometry (GC-MS)%Dibutyl phthalate (DBP)
用不同方法对菱角壳挥发油进行提取，比较邻苯二甲酸二丁酯（ DBP）的提取效果，为菱角壳挥发油化学成分分析提供参考依据。首先用水蒸气蒸馏法（ SD）和石油醚萃取法提取挥发油，用气相色谱—质谱联合分析法（ GC—MS）对提取物进行分析。结果显示：水蒸气蒸馏法与石油醚萃取法提取菱角壳挥发油获得的化合物分别有19种和10种，用水蒸气蒸馏法提取的化合物中含量最多的物质是1-甲基-2-吡咯烷酮，邻苯二甲酸二丁酯相对含量是3．60％，石油醚萃取法提取的化合物中含量最多的物质是正二十四烷，邻苯二甲酸二丁酯相对含量是9．61％。用石油醚萃取法能够从菱角壳中获得较高产率的具有抑制肿瘤细胞生长的邻苯二甲酸二丁酯（ DBP）。
用不同方法對蔆角殼揮髮油進行提取，比較鄰苯二甲痠二丁酯（ DBP）的提取效果，為蔆角殼揮髮油化學成分分析提供參攷依據。首先用水蒸氣蒸餾法（ SD）和石油醚萃取法提取揮髮油，用氣相色譜—質譜聯閤分析法（ GC—MS）對提取物進行分析。結果顯示：水蒸氣蒸餾法與石油醚萃取法提取蔆角殼揮髮油穫得的化閤物分彆有19種和10種，用水蒸氣蒸餾法提取的化閤物中含量最多的物質是1-甲基-2-吡咯烷酮，鄰苯二甲痠二丁酯相對含量是3．60％，石油醚萃取法提取的化閤物中含量最多的物質是正二十四烷，鄰苯二甲痠二丁酯相對含量是9．61％。用石油醚萃取法能夠從蔆角殼中穫得較高產率的具有抑製腫瘤細胞生長的鄰苯二甲痠二丁酯（ DBP）。
용불동방법대릉각각휘발유진행제취，비교린분이갑산이정지（ DBP）적제취효과，위릉각각휘발유화학성분분석제공삼고의거。수선용수증기증류법（ SD）화석유미췌취법제취휘발유，용기상색보—질보연합분석법（ GC—MS）대제취물진행분석。결과현시：수증기증류법여석유미췌취법제취릉각각휘발유획득적화합물분별유19충화10충，용수증기증류법제취적화합물중함량최다적물질시1-갑기-2-필각완동，린분이갑산이정지상대함량시3．60％，석유미췌취법제취적화합물중함량최다적물질시정이십사완，린분이갑산이정지상대함량시9．61％。용석유미췌취법능구종릉각각중획득교고산솔적구유억제종류세포생장적린분이갑산이정지（ DBP）。
The objective of this study is to use different methods to extract the volatile oil from water chestnut shell and compare the effect of extraction of Dibutyl phthalate ( DBP) and provides the reference for the analysis of chem-ical constituents of volatile oil from water chestnut shell.Methods:First of all,the volatile oil extracted from Trapa bispinosa Roxb by steam distillation ( SD) and petroleum ether extraction is analyzed by gas chromatography-mass spectrometry ( GC-MS) .Results:The number of chemical compound which is extracted from Trapa bispinosa Roxb by steam distillation method and petroleum ether extraction were 19 and 10, The most compounds extracted from Trapa bispinosa Roxb by water steam distillation method is 1-methyl-2-pyrrolidone, The relative content of dibutyl phthalate(DBP)is 3.60%, The most compounds extracted from Trapa bispinosa Roxb by petroleum ether extraction method was the Tetracosane, the relative content of dibutyl phthalate ( DBP) is 9.61%.Conclusion: Dibutyl phthalate ( DBP) could inhibit the growth of tumor and the higher yield of Dibutyl phthalate ( DBP) can be ob-tained from Trapa bispinosa Roxb by petroleum ether extraction.
