CAJ | 학술논문

目的：：对多囊卵巢综合征( Polycystic ovary syndrome,PCOS)模型大鼠与卵巢局部及单卵泡中抗苗勒管素( Anti-mullerian hormone,AMH)表达与胰岛素抵抗( Insulin resistance,IR)之间的相关性进行研究。方法：采用DHEA皮下注射造模建立PCOS大鼠模型,然后计算胰岛素抵抗稳态模型( Homeostasis model assessment of insulin resistance,HOMA-IR)值,根据HOMA-IR值将所筛选对象分成单纯PCOS组,PCOS合并IR组和对照组,采用免疫组化法检测模型鼠、正常鼠血清AMH的表达及单卵泡及卵巢局部大鼠胰岛素受体及AMH表达。结果：PCOS大鼠无论是否合并IR LH、T、Flns及HOMA-IR明显高于对照组,两组差异有统计学意义( P<0．05)；而PCOS合并IR组T、Flns及HOMA-IR较对照组明显升高,两组差异亦有统计学意义(P<0．05)。经过Pearson 相关性分析结果显示卵巢局部AMH 表达强度与胰岛素受体表达强度无明显相关性(r=0．015,P>0．05)；而卵巢局部AMH表达与血清胰岛素明显相关性(r=0．671,P=0．003)；单卵泡AMH表达强度与胰岛素受体表达强度无明显相关性(r=0．011,P>0．05)。而对血清AMH的表达与HOMA-IR进行Pearson相关性分析结果显示：HOMA-IR与血清 AMH 之间存在明显的正相关性(r=0．683,P=0．001)。结论：PCOS大鼠AMH与血清胰岛素水平呈正相关,卵巢局部AMH表达与胰岛素受体有正相关性,而单卵泡AMH表达与胰岛素受体无明显相关性,对于是否与高雄激素有关,还需要进一步研究。
목적：：대다낭란소종합정( Polycystic ovary syndrome,PCOS)모형대서여란소국부급단란포중항묘륵관소( Anti-mullerian hormone,AMH)표체여이도소저항( Insulin resistance,IR)지간적상관성진행연구。방법：채용DHEA피하주사조모건립PCOS대서모형,연후계산이도소저항은태모형( Homeostasis model assessment of insulin resistance,HOMA-IR)치,근거HOMA-IR치장소사선대상분성단순PCOS조,PCOS합병IR조화대조조,채용면역조화법검측모형서、정상서혈청AMH적표체급단란포급란소국부대서이도소수체급AMH표체。결과：PCOS대서무론시부합병IR LH、T、Flns급HOMA-IR명현고우대조조,량조차이유통계학의의( P<0．05)；이PCOS합병IR조T、Flns급HOMA-IR교대조조명현승고,량조차이역유통계학의의(P<0．05)。경과Pearson 상관성분석결과현시란소국부AMH 표체강도여이도소수체표체강도무명현상관성(r=0．015,P>0．05)；이란소국부AMH표체여혈청이도소명현상관성(r=0．671,P=0．003)；단란포AMH표체강도여이도소수체표체강도무명현상관성(r=0．011,P>0．05)。이대혈청AMH적표체여HOMA-IR진행Pearson상관성분석결과현시：HOMA-IR여혈청 AMH 지간존재명현적정상관성(r=0．683,P=0．001)。결론：PCOS대서AMH여혈청이도소수평정정상관,란소국부AMH표체여이도소수체유정상관성,이단란포AMH표체여이도소수체무명현상관성,대우시부여고웅격소유관,환수요진일보연구。
Objective:To study the correlation between the expression of anti-mullerian hormone( AMH) and insulin resistance (IR)degree in serum and local ovary and single follicle induced by polycystic ovary syndrome(PCOS)model. Methods: PCOS rat model was constructed by DHEA method. and then calculate the homeostasis model assessment of insulin resistance( HOMA-IR) val-ues. according to HOMA-IR value ,the object is simple screening PCOS group,PCOS merger IR group and control group were detected by immunohistochemistry model mice, the expression of AMH and insulin receptor in serum and single follicle and ovary were checked. Results:The serum FINS level and HOMA-IR was significantly higher in PCOS with IR group compared with that in control group and PCOS without IR group(P<0. 05);while the combined IR PCOS group T,Flns and HOMA-IR was significantly higher in the control group(P <0. 05);Pearson correlation analysis showed that local ovarian AMH expression of insulin receptor expression was no significant correlation(r=0. 015,P>0. 05);ovary AMH expression and serum insulin significantly was correlated(r=0. 671,P=0. 003),a single follicle AMH expression of insulin receptor expression was no significant correlation(r=0. 011,P>0. 05);HOMA-IR and serum AMH had significant positive correlation(r=0. 683,P=0. 001). Conclusion: AMH were positively correlated with serum insulin levels in PCOS rat. The expression of AMH in partial ovarian was positively correlated with the insulin receptor, while the expression of AMH in single follicle was no significant correlation with the insulin receptor. Howerer,whether the expression of AMH is correlation with hyperandrogenism has need further study.