CAJ | 학술논문

目的：：探讨非综合征型唇腭裂患儿血清丙型肝炎病毒抗体(抗-HCV)化学发光法( CLIA)出现假阳性的原因。方法：选取4050例非综合征型唇腭裂患儿作为病例组，以同期的8547例手术患儿为对照组，先采用CLIA检测抗-HCV；有反应性样本采用免疫印迹法( RIBA)及荧光定量PCR( FQ-PCR)确证；病例组有反应性病例又采用CLIA进行抗-HCV追踪监测至转阴；分析比较其转阴前后血清免疫球蛋白G( IgG)、类风湿因子( RF)及自身抗体( ANA)等检测结果的差异。结果：病例组抗-HCV有反应性样本的检出率及假阳性率均高于对照组，对比差异有统计学意义( P<0．05)；病例组检出的42例抗-HCV弱反应性样本均为假阳性，经1个月~19个月追踪监测均转为阴性，转阴前后血清IgG、RF、ANA等检测结果，对比差异无统计学意义(P>0．05)。结论：非综合征型唇腭裂病例抗-HCV CLIA法出现假阳性的原因可能与其血浆代谢异常产物有关，可通过追踪监测加以排除。
목적：：탐토비종합정형진악렬환인혈청병형간염병독항체(항-HCV)화학발광법( CLIA)출현가양성적원인。방법：선취4050례비종합정형진악렬환인작위병례조，이동기적8547례수술환인위대조조，선채용CLIA검측항-HCV；유반응성양본채용면역인적법( RIBA)급형광정량PCR( FQ-PCR)학증；병례조유반응성병례우채용CLIA진행항-HCV추종감측지전음；분석비교기전음전후혈청면역구단백G( IgG)、류풍습인자( RF)급자신항체( ANA)등검측결과적차이。결과：병례조항-HCV유반응성양본적검출솔급가양성솔균고우대조조，대비차이유통계학의의( P<0．05)；병례조검출적42례항-HCV약반응성양본균위가양성，경1개월~19개월추종감측균전위음성，전음전후혈청IgG、RF、ANA등검측결과，대비차이무통계학의의(P>0．05)。결론：비종합정형진악렬병례항-HCV CLIA법출현가양성적원인가능여기혈장대사이상산물유관，가통과추종감측가이배제。
Objective:To investigate the causes of false-positive anti-HCV in non-syndromic cleft lip and palate with serum chemiluminescence test. Methods:We performed a hospital-based,case-control study of 4 050 patients with non-syndromic cleft lip and palate and 8 547 control patients without cleft lip and palate to evaluate associations between positive anti-HCV and non-syndromic cleft lip and palate. CLIA test were used for detect anti-HCV in control group. RIBA and FQ-PCR were used for confirming the result of the positive samples of CLIA test. CLIA test were used for detect anti-HCV in case group until CLIA test was negative. IgG,RF and ANA were used for case-control comparisons. Results:The detection rate of anti-HCV were higher in the case group than that of control group (P<0. 05). The false-positive rate was 1 percent based on testing of 4 050 patients. These samples were negative after opration 1-19 months. There were no change among IgG, RF and ANA in the false-positivepatients. Conclusion: False-positive anti-HCV in non-syndromic cleft lip was possible associated with abnormal plasma metabolic product. It can be excluded by track monitoring.