CAJ | 학술논문

目的：：探讨金凤颗粒对痛风性关节炎兔关节软骨MMP-1/TIMP-1表达的影响，明确治疗痛风性关节炎作用机制。方法：采用乙胺丁醇、腺嘌呤联合灌胃+MSU关节腔注射法复制家兔痛风性关节炎模型。成模后，随机分为模型组，金凤颗粒高、中、低剂量组(19．6、98、4．9 g/kg)，别嘌醇组(0．028 g/kg)，痛风定组(0．448 g/kg)，另设空白组。连续7 d给药。末次给药2 h后，取各组兔的关节软骨组织，观察其病理形态学改变；并采用免疫细胞化学技术检测其MMP-1、TIMP-1表达，计算MMP-1/TIMP-1比值。结果：金凤颗粒可明显减轻兔关节软骨的炎性细胞浸润、充血、肿胀和坏死，可显著抑制MMP-1的表达(P<0．05)、促进TIMP-1的活化(P<0．01)，并显著降低MMP-1/TIMP-1比值(P<0．05)。结论：金凤颗粒可有效抑制痛风性关节炎的病理损害，其作用机制可能与其抑制MMP-1的表达、上调TIMP-1的表达，调节MMP-1/TIMP-1平衡有关，进而发挥抗炎作用。
목적：：탐토금봉과립대통풍성관절염토관절연골MMP-1/TIMP-1표체적영향，명학치료통풍성관절염작용궤제。방법：채용을알정순、선표령연합관위+MSU관절강주사법복제가토통풍성관절염모형。성모후，수궤분위모형조，금봉과립고、중、저제량조(19．6、98、4．9 g/kg)，별표순조(0．028 g/kg)，통풍정조(0．448 g/kg)，령설공백조。련속7 d급약。말차급약2 h후，취각조토적관절연골조직，관찰기병리형태학개변；병채용면역세포화학기술검측기MMP-1、TIMP-1표체，계산MMP-1/TIMP-1비치。결과：금봉과립가명현감경토관절연골적염성세포침윤、충혈、종창화배사，가현저억제MMP-1적표체(P<0．05)、촉진TIMP-1적활화(P<0．01)，병현저강저MMP-1/TIMP-1비치(P<0．05)。결론：금봉과립가유효억제통풍성관절염적병리손해，기작용궤제가능여기억제MMP-1적표체、상조TIMP-1적표체，조절MMP-1/TIMP-1평형유관，진이발휘항염작용。
Objective:To explore the effect of Jinfeng particles on the expression of MMP-1/TIMP-1 in cartilage of gout arthritis rabbit′s articular,to explicit treatment mechanism of gouty arthritis. Methods:Perfusion of ethambutol and adenine,inject MSU into joint cavity of rabbits to copy the model of gouty arthritis. After successful mode,were randomly divided into model group,Jinfeng granules in high,medium and low dose group(19. 6,9. 8,4. 9 g/kg),allopurinol group(0. 028 g/kg),Tongfengding group(0. 448 g/kg) ,another blank group. Administered for 7 days. 2 h after the last administration,were taken and painted articular cartilage tissue,to observe the changes of the pathological morphology;and the use of immune cell detection of the expression of TIMP-1,MMP-1,MMP-1/TIMP-1 ratio calculation. Results: Jinfeng granule could obviously reduce the articular cartilage of rats with inflammatory cells infiltration,hyperemia, swelling and necrosis, could significantly inhibit the expression of MMP-1 ( P< 0. 05 ) , stimulate TIMP-1 activation(P< 0. 01),and significantly reduce the ratio of MMP-1/TIMP-1(P< 0. 05). Conclusion: Jinfeng particles can effectively inhibit the pathological damage of gouty arthritis,upside the expression of TIMP-1 and its mechanism may be related to the inhibition of MMP-1,regulated the balance of MMP-1/TIMP-1,and then exert anti-inflammatory effects.