CAJ | 학술논문

目的：：探讨小鼠辐射损伤后T细胞亚群免疫重建特点及山茱萸的调节作用。方法：采用X射线2．6 Gy单次全身照射建立小鼠辐射损伤模型及山茱萸实验模型,分别在辐射前后,检测小鼠血常规变化；应用流式细胞术检测CD3+、CD4+和CD8+ T细胞及其Th1、Tc1、Th2、Th17和Treg亚群的比例。结果：辐射后第3天,外周血和脾脏中淋巴细胞总数及T细胞(包括CD4+、CD8+)比例显著降低(P<0．05)；T细胞在辐射后第5天开始重建,其中以CD8+T细胞为主,CD4+T细胞在第8天恢复重建。但T细胞分泌IFN-γ能力(Th1/Tc1)低于正常对照组(P<0．05)。相反,Th2、Th17、Treg亚群比例显著增高(P<0．05)。与照射组相比,山茱萸处理小鼠Th1亚群比例明显上调(P<0．05),Th2、Th17、Treg亚群的比例或数量显著下降(P<0．05)。结论：X射线单次照射后,CD8+T细胞免疫重建早于CD4+T细胞,但IFN-γ分泌能力较弱。山茱萸可显著上调Th1比例,抑制Th2、Th17、Treg增殖,改善辐射诱导T细胞亚群失衡,增强辐射损伤后Th1类细胞亚群的免疫重建优势。
목적：：탐토소서복사손상후T세포아군면역중건특점급산수유적조절작용。방법：채용X사선2．6 Gy단차전신조사건립소서복사손상모형급산수유실험모형,분별재복사전후,검측소서혈상규변화；응용류식세포술검측CD3+、CD4+화CD8+ T세포급기Th1、Tc1、Th2、Th17화Treg아군적비례。결과：복사후제3천,외주혈화비장중림파세포총수급T세포(포괄CD4+、CD8+)비례현저강저(P<0．05)；T세포재복사후제5천개시중건,기중이CD8+T세포위주,CD4+T세포재제8천회복중건。단T세포분비IFN-γ능력(Th1/Tc1)저우정상대조조(P<0．05)。상반,Th2、Th17、Treg아군비례현저증고(P<0．05)。여조사조상비,산수유처리소서Th1아군비례명현상조(P<0．05),Th2、Th17、Treg아군적비례혹수량현저하강(P<0．05)。결론：X사선단차조사후,CD8+T세포면역중건조우CD4+T세포,단IFN-γ분비능력교약。산수유가현저상조Th1비례,억제Th2、Th17、Treg증식,개선복사유도T세포아군실형,증강복사손상후Th1류세포아군적면역중건우세。
Objective:To explore the characteristics of immunological reconstitution of T-cell subsets and the role of Cornus,a Chinese medicinal herb on T lymphocytes in mice after irradiation (IR). Methods:Irradiated model mice were exposed to a single dose of X-ray radiation (2. 6 Gy) with or without Cornus treatments. Bloodroutine was examined before or after irradiation. CD3+,CD4+, CD8+ T cells and Th1, Tc1, Th2, Th17, Treg from spleen or peripheral blood were determined by flow cytometry. Results: Total lymphocytes and CD3+T cells including CD4+T and CD8+T cells,were significantly reduced 3 days after irradiation (P<0. 05). The re-constitution of CD3+ T cells ( especially CD8+T cells ) started from 5 days post irradiation, CD4+T cells increased 8 days after irradiation. However,the production of IFN-γ by Th1 or Tc1 cells were evidently decreased compared with control group even 8 days post irradiation (P<0. 05). In contrast to Th1,Th2/Th17/Treg were observably increased in irradiation group (P<0. 05). But the percentage of Th1 was obviously increased, and Th2, Th17, Treg were markedly decreased in cornus treated mice compared with irradiated mice (P<0. 05). Conclusion: The immunological reconstitution of T lymphocytes started from 5 days after total irradiation with rapid recovery of CD8+ T cells but not CD4+T cells. Cornus effectively improved the imbalance of T cell subsets by promoting the proliferation of Th1 and suppressing Th17 and Treg.