东南大学学报(医学版)
東南大學學報(醫學版)
동남대학학보(의학판)
JOURNAL OF SOUTHEAST UNIVERSITY(MEDICAL SCIENCE EDITION)
2015年
3期
383-386
,共4页
李志刚%郝林%范涛%庞昆%韩从辉
李誌剛%郝林%範濤%龐昆%韓從輝
리지강%학림%범도%방곤%한종휘
肿瘤坏死因子凋亡诱导配体%前列腺癌%膀胱癌%增殖%凋亡
腫瘤壞死因子凋亡誘導配體%前列腺癌%膀胱癌%增殖%凋亡
종류배사인자조망유도배체%전렬선암%방광암%증식%조망
tumor necrosis factor-related apoptosis ligand%prostate cancer%bladder cancer%proliferation%apoptosis
目的:研究重组并纯化的肿瘤坏死因子凋亡诱导配体( TRAIL)蛋白对前列腺癌及膀胱癌细胞增殖及凋亡的影响。方法:将不同浓度的TRAIL蛋白与培养的前列腺癌细胞( PC-3细胞)和膀胱癌细胞(5637细胞)共孵育24 h后,采用细胞增殖实验及流式细胞术检测肿瘤细胞的增殖及凋亡。结果:PC-3细胞株中,与未处理组比较,TRAIL蛋白浓度在20、40、80、160 ng · ml -1时细胞增殖率均有显著性差异;而在5637细胞株中,与未处理组比较,TRAIL蛋白浓度在5、10、20、40 ng · ml -1时细胞增殖率均有显著性差异。流式细胞实验结果也证实,随TRAIL蛋白浓度的增高,两种肿瘤细胞凋亡率增加。结论:重组并纯化TRAIL蛋白可通过抑制前列腺癌及膀胱癌细胞的增殖,诱导其凋亡而产生抗肿瘤的作用。
目的:研究重組併純化的腫瘤壞死因子凋亡誘導配體( TRAIL)蛋白對前列腺癌及膀胱癌細胞增殖及凋亡的影響。方法:將不同濃度的TRAIL蛋白與培養的前列腺癌細胞( PC-3細胞)和膀胱癌細胞(5637細胞)共孵育24 h後,採用細胞增殖實驗及流式細胞術檢測腫瘤細胞的增殖及凋亡。結果:PC-3細胞株中,與未處理組比較,TRAIL蛋白濃度在20、40、80、160 ng · ml -1時細胞增殖率均有顯著性差異;而在5637細胞株中,與未處理組比較,TRAIL蛋白濃度在5、10、20、40 ng · ml -1時細胞增殖率均有顯著性差異。流式細胞實驗結果也證實,隨TRAIL蛋白濃度的增高,兩種腫瘤細胞凋亡率增加。結論:重組併純化TRAIL蛋白可通過抑製前列腺癌及膀胱癌細胞的增殖,誘導其凋亡而產生抗腫瘤的作用。
목적:연구중조병순화적종류배사인자조망유도배체( TRAIL)단백대전렬선암급방광암세포증식급조망적영향。방법:장불동농도적TRAIL단백여배양적전렬선암세포( PC-3세포)화방광암세포(5637세포)공부육24 h후,채용세포증식실험급류식세포술검측종류세포적증식급조망。결과:PC-3세포주중,여미처리조비교,TRAIL단백농도재20、40、80、160 ng · ml -1시세포증식솔균유현저성차이;이재5637세포주중,여미처리조비교,TRAIL단백농도재5、10、20、40 ng · ml -1시세포증식솔균유현저성차이。류식세포실험결과야증실,수TRAIL단백농도적증고,량충종류세포조망솔증가。결론:중조병순화TRAIL단백가통과억제전렬선암급방광암세포적증식,유도기조망이산생항종류적작용。
Objective: To study the effect of purified tumor necrosis factor-related apoptosis ligand on the proliferation and apoptosis of prostate cancer cell (PC-3 cell) and bladder cancer cell(5637 cell).Methods:PC-3 and 5637 cell line were incubated with different concentrations of TRAIL protein for 24 h.Then cell proliferation and flow cytometry were employed to detect the PC-3 and 5637 proliferation and apoptosis .Results:The significant difference of the cell proliferation rate was found in PC-3 cells when the TRAIL protein concentration was 20,40, 80, 160 ng · ml-1 compared with untreated group .In 5637 cells, the cell proliferation rate had significant difference when the TRAIL protein concentration was 5, 10, 20, 40 ng · ml -1 compared with untreated group . Flow cytometry results also confirmed that the apoptosis rate of two kinds of tumor cell increased with the TRAIL protein dose dependently .Conclusion:Purified tumor necrosis factor-related apoptosis ligand protein can perform the anti-tumor role by inhibiting proliferation and inducing the apoptosis of the prostate cancer and bladder cancer cells .