CAJ | 학술논문

目的：研究SF／PLCL纺丝材料复合骨髓间充质干细胞修复兔桡骨骨缺损的能力。方法：取兔骨髓间充质干细胞（ BMSCs ）并制成BMSCs／藻酸钙水凝胶。选择8只6月龄雄性新西兰兔，于双侧桡骨中段制备15 mm大段骨缺损模型。其中实验组双侧植入SF／PLCL后注入BMSCs／藻酸钙水凝胶；对照组单纯行双侧桡骨缺损手术。所有兔于术后12周行CT检查观察骨缺损修复情况，之后处死兔，大体及组织学观察骨修复情况。结果：术后12周CT及组织学检查显示：实验组纺丝材料塌陷，材料部分降解，纤维细胞长入材料内部；骨缺损断端有骨组织沿套管材料生长，但骨缺损未完成桥接。对照组缺损区域软组织填充，骨缺损断端封闭。结论：SF／PLCL套管材料虽具有良好的生物相容性，但尚不足以用于修复兔桡骨骨缺损，其成骨能力及降解能力有待进一步提升。
목적：연구SF／PLCL방사재료복합골수간충질간세포수복토뇨골골결손적능력。방법：취토골수간충질간세포（ BMSCs ）병제성BMSCs／조산개수응효。선택8지6월령웅성신서란토，우쌍측뇨골중단제비15 mm대단골결손모형。기중실험조쌍측식입SF／PLCL후주입BMSCs／조산개수응효；대조조단순행쌍측뇨골결손수술。소유토우술후12주행CT검사관찰골결손수복정황，지후처사토，대체급조직학관찰골수복정황。결과：술후12주CT급조직학검사현시：실험조방사재료탑함，재료부분강해，섬유세포장입재료내부；골결손단단유골조직연투관재료생장，단골결손미완성교접。대조조결손구역연조직전충，골결손단단봉폐。결론：SF／PLCL투관재료수구유량호적생물상용성，단상불족이용우수복토뇨골골결손，기성골능력급강해능력유대진일보제승。
Objective: To study the osteogenic potential of SF/PLCL electrospinning material composited with bone mesenchymal stem cells ( BMSCs ) in repairing the radius defection in rabbits .Methods: BMSCs were obained from rabbits and then mixed for BMSCs/Alginate hydrogel .Eight 6-month-old male New Zealand white rabbits were recruited and 15 mm defect was created in both sides of the radius .In the experimental group , SF/PLCL spinning composited with BMSCs/alginate hydrogel was implanted into the bone defect .In the control group , only bilateral surgery of radial defect was performed .Twelve weeks postoperatively , CT scan reconstruction were used to evaluate the bone formation .Then the rabbits were sacrificed and the specimens were harvested for histologic study .The specimens were embedded in paraffin , followed by decalcification and then stained by hematoxylin and eosin ( HE ) for histomorphometric analysis to evaluate the bone repair .Results:Twelve weeks postoperatively , CT scan reconstruction and HE staining showed that SF /PLCL spinning degraded partly in the experimental group , and the bone formed along the casing stump , but the bone defect was not fully bridged;While in the control group , the defect was filled with soft tissue , and the ends of bone defects were closed .Conclusion:SF/PLCL casing material in this study shows good biocompatibility , but its osteogenic ability and degradation need to be further refined .