武汉轻工大学学报
武漢輕工大學學報
무한경공대학학보
Journal of Wuhan Polytechnic University
2015年
2期
43-46
,共4页
陈中强%翟莹%尚萍萍%左正宇%李琦%刘志国
陳中彊%翟瑩%尚萍萍%左正宇%李琦%劉誌國
진중강%적형%상평평%좌정우%리기%류지국
NAFLD%胆固醇%脂质聚积
NAFLD%膽固醇%脂質聚積
NAFLD%담고순%지질취적
NAFLD%cholesterol%lipid accumulation
研究胆固醇含量对高脂诱导的非酒精性脂肪肝病( NAFLD)的影响。对成年SD雄性大鼠随机分为正常组( CON)和高脂饮食诱导NAFLD组( HF),HF组下分HFC0、HFC1、HFC2和HFC5组,分别对应饲料中添加0%、1%、2%和5%的胆固醇,各组于饲养4周和8周结束时断头取血,检测血清TC、TG含量和ALT、AST活性,对8周的肝组织切片HE染色。结果显示与对照组相比,随着胆固醇摄入量增加,饲喂8周的大鼠体重、肝重及肝比重增加明显;肝形态改变明显,细胞脂质聚积和炎性浸润显著;HFC2、HFC5组于4周时血TC含量、ALT及AST升高显著。研究结果表明:高胆固醇饮食可加重肝脂质聚积、肝功能异常,并促进了非酒精性脂肪肝病的发展。
研究膽固醇含量對高脂誘導的非酒精性脂肪肝病( NAFLD)的影響。對成年SD雄性大鼠隨機分為正常組( CON)和高脂飲食誘導NAFLD組( HF),HF組下分HFC0、HFC1、HFC2和HFC5組,分彆對應飼料中添加0%、1%、2%和5%的膽固醇,各組于飼養4週和8週結束時斷頭取血,檢測血清TC、TG含量和ALT、AST活性,對8週的肝組織切片HE染色。結果顯示與對照組相比,隨著膽固醇攝入量增加,飼餵8週的大鼠體重、肝重及肝比重增加明顯;肝形態改變明顯,細胞脂質聚積和炎性浸潤顯著;HFC2、HFC5組于4週時血TC含量、ALT及AST升高顯著。研究結果錶明:高膽固醇飲食可加重肝脂質聚積、肝功能異常,併促進瞭非酒精性脂肪肝病的髮展。
연구담고순함량대고지유도적비주정성지방간병( NAFLD)적영향。대성년SD웅성대서수궤분위정상조( CON)화고지음식유도NAFLD조( HF),HF조하분HFC0、HFC1、HFC2화HFC5조,분별대응사료중첨가0%、1%、2%화5%적담고순,각조우사양4주화8주결속시단두취혈,검측혈청TC、TG함량화ALT、AST활성,대8주적간조직절편HE염색。결과현시여대조조상비,수착담고순섭입량증가,사위8주적대서체중、간중급간비중증가명현;간형태개변명현,세포지질취적화염성침윤현저;HFC2、HFC5조우4주시혈TC함량、ALT급AST승고현저。연구결과표명:고담고순음식가가중간지질취적、간공능이상,병촉진료비주정성지방간병적발전。
To research the effect of cholesterol content on the nonalcoholic fatty liver disease ( NAFLD) induced by high fat.Method:The adult male SD rats were randomly divided into the normalgroup ( CON) and high-fat diet group (HF).Then the HF group was divided into HFC0, HFC1, HFC2 and HFC5 group, and 0%, 1%, 2%and 1%cholesterol were added into the five HFC groups correspondingly.All rats were beheaded at the 4th and 8th week, and the organ blood was collected to detect the contentsof the totalcholesterol (TC), the total triglyceride (TG) and the activities of Alanineaminotransferase (AST), Alanine transaminase(ALT).The 8th weeks’ liver tis-sues embedded inparaffin were stained with hematoxylin and eosin ( H&E) to judge inflammatorysituation.Result:After 8 weeks’ feeding,compared with the CON group, the body weight, liver weight and the proportion of liver and body weight of the HFC group rats increased significantly with the increase of cholesterol intake .And the liver mor-phology changes, lipid accumulation and inflammatory infiltration could be easily observed on HFD liver cells.At the 4th week, the content of TC, activities of ALT and AST increased significantly in the HFC2 and HFC5 group. Conclusion:The results showed the high cholesterol diet can aggravate hepatic lipid accumulation, liver dysfunctio-nand promote the development of nonalcoholic fatty liver disease.