CAJ | 학술논문

通过比较测定酵母菌发酵上清液中残存的氨氮含量的方法，从假丝酵母（ Candida ）、酿酒酵母（Saccharomyces cerevisiae）、马克斯克鲁维酵母（Kluyveromyces marxianus）和东方伊萨酵母（ Issatchenkia orientalis）4个属共56株耐高温酵母菌中筛选得到6株氨氮同化率为70％以上的酵母菌株，其中酿酒酵母1株，假丝酵母属5株；通过进一步复筛，获得1株氨氮同化率高达81．1％的产朊假丝酵母（ C．utilis） SG607。不同属的酵母菌以及同种酵母菌不同菌株之间对氨氮的同化率均有明显差异。发酵实验结果表明，在培养基碳／氮比为15：1，发酵时间为24 h时，产朊假丝酵母对氨氮的同化率最高。
통과비교측정효모균발효상청액중잔존적안담함량적방법，종가사효모（ Candida ）、양주효모（Saccharomyces cerevisiae）、마극사극로유효모（Kluyveromyces marxianus）화동방이살효모（ Issatchenkia orientalis）4개속공56주내고온효모균중사선득도6주안담동화솔위70％이상적효모균주，기중양주효모1주，가사효모속5주；통과진일보복사，획득1주안담동화솔고체81．1％적산원가사효모（ C．utilis） SG607。불동속적효모균이급동충효모균불동균주지간대안담적동화솔균유명현차이。발효실험결과표명，재배양기탄／담비위15：1，발효시간위24 h시，산원가사효모대안담적동화솔최고。
In this study, six yeast strains of ammonia assimilation rate more than 70% were screened out from 56 strains of high temperature resistant yeast including Canad ida, Saccharomyces cerevisiae, Kluyveromyces marxianus and Issatchenkia orientalis by the comparison of residual ammonia nitrogen content in the supernatant of yeast fer-mentation.One of them belong to Saccharomyces cerevisiae, the other five strains belong to Candida tropicali. Through further screening, one strain of Candida utilis named SG607 with ammonia nitrogen assimilation rate as high as 81.1%was screened out.There were significantly different on assimilation rate of ammonia among different genera of yeasts and different strains of one yeast species.The fermentation experiment results showed that the Can-dida tropicali strain could reached the highest rate of ammonia assimilation when the ratio of carbon and nitrogen in the medium was 15:1, and the fermentation time was 24h.