CAJ | 학술논문

目的：探讨壬基酚对卵巢癌细胞 HO8910增殖的作用。方法 HO8910细胞在有酚红 RPMI1640（含10%新生牛血清）培养液中常规培养，实验前1天改为无酚红 RPMI1640（含5% CDT - FBS）中继续培养，1×10-5 mol/ L，1×10-6 mol/ L，1×10-7 mol/ L 的 NP 和1×10-11 mol/ L 的 E2处理细胞24 h 后，MTT 法和流式细胞术检测对HO8910细胞的增殖作用。结果1×10-5 mol/ L，1×10-6 mol/ LNP 组和 E2组细胞增殖率和细胞增殖指数均有明显增加。结论壬基酚对 HO8910细胞发挥雌激素样作用，有促进卵巢癌细胞增殖的作用。
목적：탐토임기분대란소암세포 HO8910증식적작용。방법 HO8910세포재유분홍 RPMI1640（함10%신생우혈청）배양액중상규배양，실험전1천개위무분홍 RPMI1640（함5% CDT - FBS）중계속배양，1×10-5 mol/ L，1×10-6 mol/ L，1×10-7 mol/ L 적 NP 화1×10-11 mol/ L 적 E2처리세포24 h 후，MTT 법화류식세포술검측대HO8910세포적증식작용。결과1×10-5 mol/ L，1×10-6 mol/ LNP 조화 E2조세포증식솔화세포증식지수균유명현증가。결론임기분대 HO8910세포발휘자격소양작용，유촉진란소암세포증식적작용。
Objective:To investigate the effect of nonylphenol on ovarian cancer HO8910 cells proliferation. Method:HO8910 cells was maintained in RPMI1640 medium with 10% neonatal bowine serum. 1d before the beginning of the exper-iment ,the cells were be seeded in phenol red - free 1640 medium containing 5% charcoal dextran - treated FBS,after 1 × 10 - 5 mol/ L,1 × 10 - 6 mol/ L and 1 × 10 - 7 mol/ L of NP and 1 × 10 - 7 mol/ L E2 treatment for 24 h,the proliferation of HP8910 was analyzed by MTT and flow sytometer. Results:Cell proliferation rate and Cell proliferation index increased sig-nificantly in 1 × 10 - 5 mol/ L,1 × 10 - 6 mol/ LNP groups and E2 group. Conclusion:NP produce similar effects on HO8910 with E2 ,maybe promote the proliferation ovarian cancer cell.