CAJ | 학술논문

目的：建立高效液相色谱（HPLC‐DAD）方法，对鲜洋葱中的主要黄酮类化合物进行含量测定。方法采用色谱柱Shimadzu VP‐ODS （250mm ×4．6 mm ，5μm），以乙腈－水为流动相，梯度洗脱，柱温30℃，流速1．0 ml／min ，检测波长350 nm。首先根据紫外光谱图，确定黄酮类化学成分。而后以芦丁为对照品建立标准曲线，对洋葱中黄酮类化学成分进行定量研究。结果建立的高效液相色谱分析方法能够满足洋葱中主要黄酮类化学成分准确测定的要求，芦丁在5．0～502．5μg／ml范围内有良好的线性关系（r＝0．9995），重复性（RSD＜4.1％），日内精密度和日间精密度（RSD＜3．3 ％），加样回收率（95．1％～103．2 ％），检出限（0．3μg／ml）和定量限（1．0μg／ml）。含量测定表明洋葱中主要黄酮类化学成分的含量为2．8 mg／g。结论该方法简便可行，测定结果准确，能够满足洋葱中主要黄酮类化学成分准确测定的需要。
목적：건립고효액상색보（HPLC‐DAD）방법，대선양총중적주요황동류화합물진행함량측정。방법채용색보주Shimadzu VP‐ODS （250mm ×4．6 mm ，5μm），이을정－수위류동상，제도세탈，주온30℃，류속1．0 ml／min ，검측파장350 nm。수선근거자외광보도，학정황동류화학성분。이후이호정위대조품건립표준곡선，대양총중황동류화학성분진행정량연구。결과건립적고효액상색보분석방법능구만족양총중주요황동류화학성분준학측정적요구，호정재5．0～502．5μg／ml범위내유량호적선성관계（r＝0．9995），중복성（RSD＜4.1％），일내정밀도화일간정밀도（RSD＜3．3 ％），가양회수솔（95．1％～103．2 ％），검출한（0．3μg／ml）화정량한（1．0μg／ml）。함량측정표명양총중주요황동류화학성분적함량위2．8 mg／g。결론해방법간편가행，측정결과준학，능구만족양총중주요황동류화학성분준학측정적수요。
Objective To establish a high performance liquid chromatography coupled with a diode array detector (HPLC‐DAD) method for the determination of major flavonoids in onion .Methods The chromatographic separation was performed in a SHIMADZU VP‐ODS (250mm × 4.6mm ,5 μm) column .The mobile phase was composed of wa‐ter (A) and acetonitrile (B) using gradient elution . The column temperature was 30℃ with the flow rate 1.0 ml/min ,and UV absorbance was detected at a wavelength of 350 nm . Firstly , the flavonoids were identified by maximum UV absorption wavelength .Then ,the rutin was used as a standard substance to establish the standard curve to determine the major flavonoids in onion .Results The HPLC‐DAD method was established and validated to determine the major flavonoids .The calibration curve was linear in the range of 5 .0~502 .5μg/ml with good linear‐ity ( r=0.9995) ,repeatability(RSD<4 .1% ) ,intra‐day and inter‐day precision(RSD<3 .3% ) ,recovery(95 .1% ~103.2% ) ,limit of detection (0 .3μg/ml) ,and limit of quantification (1 .0μg/ml) .The content of flavonoids in on‐ion was 2 .8 mg/g .Conclusion The method is convenient ,feasible ,and accurate for the determination of major fla‐vonoids in onion .