CAJ | 학술논문

为明确用茉莉酸不同处理方式对林木诱导抗虫性的影响，以长白落叶松（ Larix olgensis）幼苗为试验材料，分别用浓度为1．00、0．10、0．01 mmol · L－1的茉莉酸进行全株或局部喷施诱导，分析不同处理对其针叶内SOD、PPO、PAL、CI、TI 5种防御蛋白活力的影响。结果表明：用外源茉莉酸局部喷施长白落叶松，可以系统诱导增强其防御蛋白的活性，且诱导效果与全株喷施处理的诱导效果相近。0．10、1．00 mmol· L－1的茉莉酸在1～10 d内即可诱导产生较强的诱导抗性，而在15～25 d时其诱导的抗性强度显著低于0．01 mmol· L－1浓度的诱导效果。说明高浓度茉莉酸能快速诱导长白落叶松产生较强的化学防御能力，低浓度茉莉酸诱导长白落叶松化学防御的速度相对迟缓，但其持效时间长于高浓度茉莉酸诱导效果。在害虫控制实践中，可根据害虫的生物特性，选择合适浓度的茉莉酸，采用局部喷施方式施用，将更为经济和便于操作。
위명학용말리산불동처리방식대림목유도항충성적영향，이장백락협송（ Larix olgensis）유묘위시험재료，분별용농도위1．00、0．10、0．01 mmol · L－1적말리산진행전주혹국부분시유도，분석불동처리대기침협내SOD、PPO、PAL、CI、TI 5충방어단백활력적영향。결과표명：용외원말리산국부분시장백락협송，가이계통유도증강기방어단백적활성，차유도효과여전주분시처리적유도효과상근。0．10、1．00 mmol· L－1적말리산재1～10 d내즉가유도산생교강적유도항성，이재15～25 d시기유도적항성강도현저저우0．01 mmol· L－1농도적유도효과。설명고농도말리산능쾌속유도장백락협송산생교강적화학방어능력，저농도말리산유도장백락협송화학방어적속도상대지완，단기지효시간장우고농도말리산유도효과。재해충공제실천중，가근거해충적생물특성，선택합괄농도적말리산，채용국부분시방식시용，장경위경제화편우조작。
We used Larix olgensis seedlings to test the effects of different jasmonic acid ( JA) treatments on the induced tree re-sistance against insect attacks.We sprayed three concentrations of JA (1, 0.1 and 0.01 mmol/L), respectively, on the whole larch seedling or on part of the seedling plant , and measured their effects on enzyme activity of following major de-fense proteins (superoxide dismutase, SOD, Polyphenol oxidase, PPO, phenylalanine ammonia-lyase, PAL, chymotryp-sin inhibitor, CI, trypsin inhibitor, TI).The topical spray of exogenous jasmonic acid on part of L.olgensis seedling plant enhanced its induced defense system by increasing activities of the defense proteins at similar levels , so did the whole plant application.Jasmonic acid resulted in a strong induced resistance at 0.1or 1 mmol/L within 1-10 d of application, but their effects were significantly lower than that at the low application concentration (0.01 mmol/L) winthin 15-25 d.The high concentrations of JA could increase the induced resistance level of larch seedlings strongly and quickly , whereas, low concentration might induce the resistance slowly but sustain the resistance level longer than the higher concentrations .In practice, partial spray of low concentrations of jasmonic acid on L.olgensis seedlings or trees might be an economical and efficient option.