CAJ | 학술논문

目的观察鼠类肉瘤病毒癌基因(KRAS)和表皮生长因子受体(EGFR)在非小细胞肺癌(NSCLC)原发病灶和转移淋巴结中的表达,探讨KRAS和EGFR在NSCLC淋巴结转移中的作用.方法收集经手术切除且病理证实的186例NSCLC患者,记录其性别、年龄、肿瘤性质、浸润程度、淋巴结转移情况及TNM分期等各项临床相关资料.将肿瘤标本行免疫组织化学染色,并提取肿瘤细胞DNA行聚合酶链反应(PCR)扩增和测序,观察EGFR和KRAS在NSCLC原发病灶和转移淋巴结中的表达及变异.结果 (1)EGFR蛋白在NSCLC组织中表达阳性率为34.41％(64例),KRAS在NSCLC组织中表达阳性率为36.56％(68例).EGFR和KRAS阳性表达与淋巴结转移明显相关(P＜0.05).(2)原发病灶与淋巴结转移病灶间KRAS基因突变率差异有统计学意义(P＜0.05),EGFR突变率差异无统计学意义(P＞0.05).结论 EGFR和KRAS在NSCLC组织中高表达,且其表达与变异与NSCLC淋巴结转移密切相关.
목적 관찰서류육류병독암기인(KRAS)화표피생장인자수체(EGFR)재비소세포폐암(NSCLC)원발병조화전이림파결중적표체,탐토KRAS화EGFR재NSCLC림파결전이중적작용.방법 수집경수술절제차병리증실적186례NSCLC환자,기록기성별、년령、종류성질、침윤정도、림파결전이정황급TNM분기등각항림상상관자료.장종류표본행면역조직화학염색,병제취종류세포DNA행취합매련반응(PCR)확증화측서,관찰EGFR화KRAS재NSCLC원발병조화전이림파결중적표체급변이.결과 (1)EGFR단백재NSCLC조직중표체양성솔위34.41％(64례),KRAS재NSCLC조직중표체양성솔위36.56％(68례).EGFR화KRAS양성표체여림파결전이명현상관(P＜0.05).(2)원발병조여림파결전이병조간KRAS기인돌변솔차이유통계학의의(P＜0.05),EGFR돌변솔차이무통계학의의(P＞0.05).결론 EGFR화KRAS재NSCLC조직중고표체,차기표체여변이여NSCLC림파결전이밀절상관.
Objective To investigate epidermal growth factor receptor (EGFR) and kirsten rat sarcoma viral oncogene (KRAS) expression in non-small cell lung cancer (NSCLC) and lymphatic metastases,and to explore the roles of EGFR and KRAS in NSCLC lymphatic metastases.Methods By collecting surgically resected and pathologically confirmed cases of NSCLC,get 186 cases of complete clinical data,extraction of tumor cell DNA,polymerase chain reaction (PCR) amplification and sequencing.To observe EGFR and KRAS expression in NSCLC and lymphatic metastasis,and to analyse with SPSS 14.0 statistical software.Results Positive rate of EGFR gene expression in NSCLC was 34.41％ (64 cases).EGFR gene expression was positively correlated with the differentiation and lymphaden metastases.Positive rate of KRAS expression in gastric cancer was 36.56％ (68 cases).KRAS expression was positively correlated with the lymphaden metastases and clinical stages.The mutation rate of KRAS had significant difference in NSCLC and lymph node metastasis,but the mutation rate of EGFR had no significant difference.Conclusion EGFR and KRAS were high expression in NSCLC tissue,and it may give some guideline for the clinical diagnosis,illness evaluation,treatment and prognosis of gastric cancer.