CAJ | 학술논문

目的探讨12周四氯化碳腹腔注射诱导小鼠肝纤维化的效果.方法每周3次10％四氯化碳200μl腹腔注射,诱导C57BL/6小鼠肝脏纤维化.注射8、12周时处死小鼠,通过大体标本与石蜡切片镜检确定肝纤维化程度.结果注射8周小鼠肝表面有颗粒感,镜下汇管区纤维向小叶内延伸,呈中度肝纤维化,注射12周小鼠肝表面出现结节,镜下有纤维隔伴假小叶形成,为重度肝纤维化.且12周四氯化碳腹腔注射对小鼠精神、体质量无明显影响,对照组体质量为29.0g,实验组为(26.8 ±0.7)g,差异无统计学意义(P＞0.05),且实验中无小鼠死亡.结论腹腔四氯化碳注射是一种安全有效的小鼠肝纤维化模型建立方法.
목적 탐토12주사록화탄복강주사유도소서간섬유화적효과.방법 매주3차10％사록화탄200μl복강주사,유도C57BL/6소서간장섬유화.주사8、12주시처사소서,통과대체표본여석사절편경검학정간섬유화정도.결과 주사8주소서간표면유과립감,경하회관구섬유향소협내연신,정중도간섬유화,주사12주소서간표면출현결절,경하유섬유격반가소협형성,위중도간섬유화.차12주사록화탄복강주사대소서정신、체질량무명현영향,대조조체질량위29.0g,실험조위(26.8 ±0.7)g,차이무통계학의의(P＞0.05),차실험중무소서사망.결론 복강사록화탄주사시일충안전유효적소서간섬유화모형건립방법.
Objective To determine the hepatic fibrosis-induced effect of 12 week intraperitoneal injection of carbon tetrachloride in mice.Methods The hepatic fibrosis in C57BL/6 mice was induced by intraperitoneal injection of 200 μl 10％ carbon tetrachloride three times every week.At 8th and 12th week,the mice were executed.The severity of hepatic fibrosis was identified by gross observation and microscopic examination.Results Livers of mice executed at week 8 had rough surface and fibers extended to the hepatic lobule,and moderate hepatic fibrosis was induced.In the mice executed at week 12,there were nodules on the liver surface,and the structure of hepatic lobules disordered and false lobules formed,suggesting the successful induction of hepatic fibrosis.Moreover,intraperitoneal injection of 12 week carbon tetrachloride had no significant influence on activity and mean weight of mice.Mean weight in control group and experimental group was 29.0 g and (26.8 ± 0.7) g respectively,P ＞ 0.05.Conclusion Intraperitoneal injection of carbon tetrachloride in mice is a safe and effective method for building hepatic fibrosis model.