CAJ | 학술논문

观察并分析了荧光增白剂和偶联Alexa 488荧光素的小麦凝集素2种荧光染色方法对松杨栅锈菌（ Melampsora larici-populina）无性发育不同阶段的染色效果。结果表明：偶联Alexa 488荧光素的小麦凝集素染色处理后能够较清楚地观察到锈菌的夏孢子、萌发的芽管、附着胞、气孔下囊、侵染菌丝、吸器母细胞及吸器等不同发育阶段的结构，荧光增白剂处理后不易观察到锈菌的侵染菌丝、吸器母细胞及吸器等结构。因此，偶联Alexa 488荧光素的小麦凝集素荧光染色方法可以作为研究松杨栅锈菌与寄主杨树互作的一种组织病理学方法。
관찰병분석료형광증백제화우련Alexa 488형광소적소맥응집소2충형광염색방법대송양책수균（ Melampsora larici-populina）무성발육불동계단적염색효과。결과표명：우련Alexa 488형광소적소맥응집소염색처리후능구교청초지관찰도수균적하포자、맹발적아관、부착포、기공하낭、침염균사、흡기모세포급흡기등불동발육계단적결구，형광증백제처리후불역관찰도수균적침염균사、흡기모세포급흡기등결구。인차，우련Alexa 488형광소적소맥응집소형광염색방법가이작위연구송양책수균여기주양수호작적일충조직병이학방법。
We used two fluorescence staining methods , Calcofluor white and wheat germ agglutinin ( WGA) conjugated to the fluorophorealexa 488, to observe the vegetative cycle of Melampsora larici-populina ( MLP ) .The urediniospore , germ tube, appressoria, substomatal vesicle, infection hypha, haustorial mother cell and haustorium could be observed by stai-ning with WGA-alexa 488, which was a usefully histological method to study the mechanism of the interaction between MLP and poplar.However, infection hyphae, haustorial mother cell and haustorium were not easy to be observed after stai -ning with Calcofluor white .