CAJ | 학술논문

文冠果（Xanthoceras sorbifolia Bunge）根际土壤和根系内生真菌的多样性与土壤养分变化具有相关性。以人工文冠果林为研究对象，采集根际土壤和细根并提取DNA，采用Illumina Miseq高通量测序技术分析真菌rDNA的ITS1区域，同时测定了根际土壤的N、P和有机质等因子，以分析文冠果林根际土壤真菌和根系共生真菌与环境因子的关系。结果表明：①测序共获得clean reads有238．8104万个（土壤209．2113万个，根系295．9910万个）；在97％的相似度水平下，共获得1115个操作分类单元（ OTUs）：根际土壤真菌1028个，根系共生真菌514个，其中有59个OTUs鉴定为菌根真菌。在10～12年生林中，病原真菌数量较高，主要为镰刀菌属（ Fusa rium）、链格孢属（ Atl ernaria）及青霉属（ Penicillium），可能与林地土壤肥力退化有关。②不同林龄文冠果根际土壤理化性质差异显著，1～2年生林土壤肥力较高，全氮、速效氮、全磷、速效磷质量分数分别为110．00、345．6、230．00、5．18 mg· kg－1，均高于5～7年生林和10～12年生林。随土壤肥力降低根内真菌OTUs个数递减。③1～2年生林根际土壤和根系内生真菌丰富度指数Chao1、均匀度指数ACE均最高。真菌群落多样性指数Simpson与全氮、速效磷质量分数呈正相关关系。可见，不同林龄文冠果人工林真菌群落存在差异，文冠果根际真菌群落与土壤环境因子之间具有相关性。
문관과（Xanthoceras sorbifolia Bunge）근제토양화근계내생진균적다양성여토양양분변화구유상관성。이인공문관과림위연구대상，채집근제토양화세근병제취DNA，채용Illumina Miseq고통량측서기술분석진균rDNA적ITS1구역，동시측정료근제토양적N、P화유궤질등인자，이분석문관과림근제토양진균화근계공생진균여배경인자적관계。결과표명：①측서공획득clean reads유238．8104만개（토양209．2113만개，근계295．9910만개）；재97％적상사도수평하，공획득1115개조작분류단원（ OTUs）：근제토양진균1028개，근계공생진균514개，기중유59개OTUs감정위균근진균。재10～12년생림중，병원진균수량교고，주요위렴도균속（ Fusa rium）、련격포속（ Atl ernaria）급청매속（ Penicillium），가능여임지토양비력퇴화유관。②불동림령문관과근제토양이화성질차이현저，1～2년생림토양비력교고，전담、속효담、전린、속효린질량분수분별위110．00、345．6、230．00、5．18 mg· kg－1，균고우5～7년생림화10～12년생림。수토양비력강저근내진균OTUs개수체감。③1～2년생림근제토양화근계내생진균봉부도지수Chao1、균균도지수ACE균최고。진균군락다양성지수Simpson여전담、속효린질량분수정정상관관계。가견，불동림령문관과인공림진균군락존재차이，문관과근제진균군락여토양배경인자지간구유상관성。
We studied the soil fungal community and root-associated fungal community in the Xanthoceras sorbifolia Bunge plan-tation with different ages .We amplified and sequenced the inter-generic spacer 1 in the Fungal Ribosomal DNA on an Illu-mina Miseq platform, and analyzed the soil N , alkali-hydrolysable N, total P and available P .In total, 1115 OTUs con-tained 1028 fungi in the rhizosphere soil and 514 root-associated fungi , 59 of which belonged to mycorrhizal fungi .The dominant species belonged to Ascomycetes and Basidiomycetes species .There were the differences in soil physical-chemical characteristics among forests differed in age , specifically, the soil from the 2-a seedling nurse had higher total N , alkali-hydrolysable N, total P and available P than those of 5-a and 10-a forest.A high proportion of pathogenic fungi in 10-a for-est was dominated by Fusarium Alternaria and Penicillium.By correlation analysis , the diversity of soil fungi was positively correlated with total N and available P .There were different characteristics of fungi community in different ages of Xantho-ceras sorbifolia forest plantation , and the fungal diversity was significantly related to environmental factors .