CAJ | 학술논문

选取海州常山（ Clerodne drum trichotomum Thumb．）花粉为研究对象，研究了海州常山花粉活力测定的方法、最佳培养基配方及其最佳培养条件；探究了花期及花粉采集时间对海州常山花粉萌发的影响。结果表明：染色法及无机酸培养法不适宜于海州常山花粉活力的测定，最佳花粉活力测定法为液体萌发法；海州常山花粉萌发最佳培养基配方为150 g· L－1蔗糖＋200 mg· L－1硼酸＋150 mg·L －1氯化钙；其中硼酸及氯化钙pH值为5．5条件下花粉萌发效果更佳；花粉最适宜培养条件为干燥9，h 于25℃光照条件下液体培养8 h；花粉活力最强时间为花后1～2 d的08：00。
선취해주상산（ Clerodne drum trichotomum Thumb．）화분위연구대상，연구료해주상산화분활력측정적방법、최가배양기배방급기최가배양조건；탐구료화기급화분채집시간대해주상산화분맹발적영향。결과표명：염색법급무궤산배양법불괄의우해주상산화분활력적측정，최가화분활력측정법위액체맹발법；해주상산화분맹발최가배양기배방위150 g· L－1자당＋200 mg· L－1붕산＋150 mg·L －1록화개；기중붕산급록화개pH치위5．5조건하화분맹발효과경가；화분최괄의배양조건위간조9，h 우25℃광조조건하액체배양8 h；화분활력최강시간위화후1～2 d적08：00。
This experiment was conducted with C.trichotomum pollen to study the method of pollen activity assay , the best cul-ture medium formula and its optimal culture conditions and the effects of flowering and pollen collecting time on the pollen germination.The best methods of liquid staining and inorganic acid culture were not suitable for pollen activity assay , and the best method was liquid culture .The best culture medium formula for pollen germination was 150 g· L-1 sucrose+boric acid 200 mg· L-1+150 mg· L-1 calcium chloride .The effects of pollen germination would be better , if pH of boric acid and calcium chloride was5 .5.The appropriate culture conditions was as follows:drying pollen for 9 h, then putting it at 25℃with light liquid culture for 8 h.When C.trichotomumwas flowering for 1-2 d, the activity of pollen was the strongest .