心血管康复医学杂志
心血管康複醫學雜誌
심혈관강복의학잡지
JOURNAL OF CARDIOVASCULAR REHABILITATION MEDICINE
2015年
3期
231-235
,共5页
王聿杰%齐国先%黄带发%郑晓伟
王聿傑%齊國先%黃帶髮%鄭曉偉
왕율걸%제국선%황대발%정효위
半胱氨酸天冬氨酸蛋白酶3%糖尿病%大鼠%细胞凋亡
半胱氨痠天鼕氨痠蛋白酶3%糖尿病%大鼠%細胞凋亡
반광안산천동안산단백매3%당뇨병%대서%세포조망
Caspase 3%Diabetes mellitus%Rats%Apoptosis
目的：探讨慢性氯化钴处理对GK糖尿病大鼠血糖及心肌细胞凋亡的影响。方法：应用体重200～250 g的雄性GK糖尿病大鼠及其对照雄性Wistar大鼠，制作大鼠在体心梗模型，制作前测量血糖，手术成功后大鼠被分为六组：Wistar假手术组、Wistar心梗组、Wistar心梗＋氯化钴组、GK假手术组、GK心梗组和GK心梗＋氯化钴组，每组至少存活6只。3周后，测量血糖，剪取心脏，检测缺血坏死区Caspase-3凋亡基因的表达。结果：（1）术后3周，与GK假手术组及心梗组相比， GK心梗＋氯化钴处理组血糖降低[（27.73±2.58） mmol／L、（27.87±3.18） mmol／L比（16.3±2.15） mmol／L]， P均＜0.05；（2）与 GK心梗组相比， GK 心梗＋氯化钴处理组Caspase-3光密度值[（0.84±0.03）比（0.70±0.03）]及mRNA表达[（0.64±0.03）比（0.52±0.03）]显著减少，P均＜0.05；（3） Wistar心梗组和Wistar心梗＋氯化钴组血糖、Caspase-3光密度值及mRNA表达未见明显差异（P均＞0.05）。结论：慢性氯化钴处理可降低GK糖尿病大鼠血糖，减少Caspase-3凋亡基因在缺血坏死区的表达。
目的：探討慢性氯化鈷處理對GK糖尿病大鼠血糖及心肌細胞凋亡的影響。方法：應用體重200～250 g的雄性GK糖尿病大鼠及其對照雄性Wistar大鼠，製作大鼠在體心梗模型，製作前測量血糖，手術成功後大鼠被分為六組：Wistar假手術組、Wistar心梗組、Wistar心梗＋氯化鈷組、GK假手術組、GK心梗組和GK心梗＋氯化鈷組，每組至少存活6隻。3週後，測量血糖，剪取心髒，檢測缺血壞死區Caspase-3凋亡基因的錶達。結果：（1）術後3週，與GK假手術組及心梗組相比， GK心梗＋氯化鈷處理組血糖降低[（27.73±2.58） mmol／L、（27.87±3.18） mmol／L比（16.3±2.15） mmol／L]， P均＜0.05；（2）與 GK心梗組相比， GK 心梗＋氯化鈷處理組Caspase-3光密度值[（0.84±0.03）比（0.70±0.03）]及mRNA錶達[（0.64±0.03）比（0.52±0.03）]顯著減少，P均＜0.05；（3） Wistar心梗組和Wistar心梗＋氯化鈷組血糖、Caspase-3光密度值及mRNA錶達未見明顯差異（P均＞0.05）。結論：慢性氯化鈷處理可降低GK糖尿病大鼠血糖，減少Caspase-3凋亡基因在缺血壞死區的錶達。
목적：탐토만성록화고처리대GK당뇨병대서혈당급심기세포조망적영향。방법：응용체중200～250 g적웅성GK당뇨병대서급기대조웅성Wistar대서，제작대서재체심경모형，제작전측량혈당，수술성공후대서피분위륙조：Wistar가수술조、Wistar심경조、Wistar심경＋록화고조、GK가수술조、GK심경조화GK심경＋록화고조，매조지소존활6지。3주후，측량혈당，전취심장，검측결혈배사구Caspase-3조망기인적표체。결과：（1）술후3주，여GK가수술조급심경조상비， GK심경＋록화고처리조혈당강저[（27.73±2.58） mmol／L、（27.87±3.18） mmol／L비（16.3±2.15） mmol／L]， P균＜0.05；（2）여 GK심경조상비， GK 심경＋록화고처리조Caspase-3광밀도치[（0.84±0.03）비（0.70±0.03）]급mRNA표체[（0.64±0.03）비（0.52±0.03）]현저감소，P균＜0.05；（3） Wistar심경조화Wistar심경＋록화고조혈당、Caspase-3광밀도치급mRNA표체미견명현차이（P균＞0.05）。결론：만성록화고처리가강저GK당뇨병대서혈당，감소Caspase-3조망기인재결혈배사구적표체。
Objective:To explore the influence of chronic cobalt chloride (CoCl2 ) treatment on blood glucose and car-diomyocyte apoptosis in diabetic rats .Methods:In vivo MI model of rat was established using male GK diabetic rats and male Wistar rats (all weighing 200g~250g) .Blood glucose level was measured before model establishment .Af-ter successful operation ,rats were divided into six groups:Wistar sham operation group ,Wistar MI group ,Wistar MI + CoCl2 group ,GK sham operation group ,GK MI group and GK MI + CoCl2 group ,six rats survived in each group at least .After three weeks ,blood glucose level was measured and heart was excised ,expression of Caspase-3 apoptosis gene in ischemic necrosis area was measured .Results:(1) Compared with GK sham operation group and GK MI group on three weeks after operation ,there was significant reduction in blood glucose level [ (27.73 ± 2.58) mmol/L ,(27.87 ± 3.18) mmol/L vs .(16.3 ± 2.15) mmol/L] in GK MI + CoCl2 group ,P<0.05 both;(2) Compared with GK MI group ,there were significant reductions in optical density [ (0.84 ± 0.03) vs .(0.70 ± 0.03)] and mRNA ex-pression [ (0.64 ± 0.03) vs .(0.52 ± 0.03)] of Caspase-3 in GK MI + CoCl2 group ,P<0.05 both;(3) There were no significant difference in blood glucose level ,optical density and mRNA expression of Caspase-3 in Wistar MI group and Wistar MI + CoCl2 group , P>0.05 all .Conclusion:Chronic cobalt chloride treatment can decrease blood glucose level and reduce expression of Caspase-3 apoptosis gene in ischemic necrosis area in diabetic rats .
