浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
6期
538-541
,共4页
刘国涛%陈巧%金丽明%张勤
劉國濤%陳巧%金麗明%張勤
류국도%진교%금려명%장근
大鼠%肠粘连%丹参多酚酸盐%聚维酮碘%白细胞介素-1β%转化生长因子-β1
大鼠%腸粘連%丹參多酚痠鹽%聚維酮碘%白細胞介素-1β%轉化生長因子-β1
대서%장점련%단삼다분산염%취유동전%백세포개소-1β%전화생장인자-β1
rats%intestinal adhesion%salvianolate%povidone-iodine%IL-1β%TGF-β1
目的：观察丹参多酚酸盐联合聚维酮碘对大鼠术后肠粘连的影响，探讨其可能作用机制。方法选用SD雄性大鼠50只，随机分为对照组、模型组、丹参多酚酸盐组、聚维酮碘组（PVP-I组）及丹参多酚酸盐联合PVP-I组（联合组），每组10只。除对照组外，其余各组均制作肠粘连模型。对照组和模型组每天腹腔注射5%葡萄糖溶液；丹参多酚酸盐组、PVP-I组和联合组每天分别腹腔注射丹参多酚酸盐、PVP-I和二者联合，连续注射8天。大鼠于术后第7天开腹，肉眼观察各组大鼠术后粘连分级情况；酶联免疫吸附法（ELISA）检测各组大鼠血清和粘连组织白细胞介素-1β（IL-1β）、转化生长因子-β1（TGF-β1）水平。结果丹参多酚酸盐组及联合组肠粘连程度明显低于模型组（P<0.05）；丹参多酚酸盐组、PVP-I组及联合组血清和粘连组织IL-1β表达水平均显著降低于模型组[（64.82±10.55）pg/mL、（70.93±16.06）pg/mL、（62.37±3.32）pg/mL vs（75.75±6.67）pg/mL，（178.90±17.28）pg/mL、（190.76±34.16）pg/mL、（168.40±25.14）pg/mL vs（220.78±18.31）pg/mL，P<0.05或P<0.01）。丹参多酚酸盐组、PVP-I组及联合组血清和粘连组织TGF-β1表达水平均显著降低于模型组[（36.77±5.25）pg/mL、（42.67±3.40）pg/mL、（21.90±1.15）pg/mL vs（54.93±4.75）pg/mL，（639.62±118.31）pg/mL、（645.32±56.95）pg/mL、（609.87±64.88）pg/mL vs（759.87±178.42）pg/mL，P<0.05或P<0.01）。结论丹参多酚酸盐、PVP-I及二者联合均能降低大鼠术后肠粘连程度，这可能与其降低血清和粘连组织中IL-1β和TGF-β1表达水平有关。
目的：觀察丹參多酚痠鹽聯閤聚維酮碘對大鼠術後腸粘連的影響，探討其可能作用機製。方法選用SD雄性大鼠50隻，隨機分為對照組、模型組、丹參多酚痠鹽組、聚維酮碘組（PVP-I組）及丹參多酚痠鹽聯閤PVP-I組（聯閤組），每組10隻。除對照組外，其餘各組均製作腸粘連模型。對照組和模型組每天腹腔註射5%葡萄糖溶液；丹參多酚痠鹽組、PVP-I組和聯閤組每天分彆腹腔註射丹參多酚痠鹽、PVP-I和二者聯閤，連續註射8天。大鼠于術後第7天開腹，肉眼觀察各組大鼠術後粘連分級情況；酶聯免疫吸附法（ELISA）檢測各組大鼠血清和粘連組織白細胞介素-1β（IL-1β）、轉化生長因子-β1（TGF-β1）水平。結果丹參多酚痠鹽組及聯閤組腸粘連程度明顯低于模型組（P<0.05）；丹參多酚痠鹽組、PVP-I組及聯閤組血清和粘連組織IL-1β錶達水平均顯著降低于模型組[（64.82±10.55）pg/mL、（70.93±16.06）pg/mL、（62.37±3.32）pg/mL vs（75.75±6.67）pg/mL，（178.90±17.28）pg/mL、（190.76±34.16）pg/mL、（168.40±25.14）pg/mL vs（220.78±18.31）pg/mL，P<0.05或P<0.01）。丹參多酚痠鹽組、PVP-I組及聯閤組血清和粘連組織TGF-β1錶達水平均顯著降低于模型組[（36.77±5.25）pg/mL、（42.67±3.40）pg/mL、（21.90±1.15）pg/mL vs（54.93±4.75）pg/mL，（639.62±118.31）pg/mL、（645.32±56.95）pg/mL、（609.87±64.88）pg/mL vs（759.87±178.42）pg/mL，P<0.05或P<0.01）。結論丹參多酚痠鹽、PVP-I及二者聯閤均能降低大鼠術後腸粘連程度，這可能與其降低血清和粘連組織中IL-1β和TGF-β1錶達水平有關。
목적：관찰단삼다분산염연합취유동전대대서술후장점련적영향，탐토기가능작용궤제。방법선용SD웅성대서50지，수궤분위대조조、모형조、단삼다분산염조、취유동전조（PVP-I조）급단삼다분산염연합PVP-I조（연합조），매조10지。제대조조외，기여각조균제작장점련모형。대조조화모형조매천복강주사5%포도당용액；단삼다분산염조、PVP-I조화연합조매천분별복강주사단삼다분산염、PVP-I화이자연합，련속주사8천。대서우술후제7천개복，육안관찰각조대서술후점련분급정황；매련면역흡부법（ELISA）검측각조대서혈청화점련조직백세포개소-1β（IL-1β）、전화생장인자-β1（TGF-β1）수평。결과단삼다분산염조급연합조장점련정도명현저우모형조（P<0.05）；단삼다분산염조、PVP-I조급연합조혈청화점련조직IL-1β표체수평균현저강저우모형조[（64.82±10.55）pg/mL、（70.93±16.06）pg/mL、（62.37±3.32）pg/mL vs（75.75±6.67）pg/mL，（178.90±17.28）pg/mL、（190.76±34.16）pg/mL、（168.40±25.14）pg/mL vs（220.78±18.31）pg/mL，P<0.05혹P<0.01）。단삼다분산염조、PVP-I조급연합조혈청화점련조직TGF-β1표체수평균현저강저우모형조[（36.77±5.25）pg/mL、（42.67±3.40）pg/mL、（21.90±1.15）pg/mL vs（54.93±4.75）pg/mL，（639.62±118.31）pg/mL、（645.32±56.95）pg/mL、（609.87±64.88）pg/mL vs（759.87±178.42）pg/mL，P<0.05혹P<0.01）。결론단삼다분산염、PVP-I급이자연합균능강저대서술후장점련정도，저가능여기강저혈청화점련조직중IL-1β화TGF-β1표체수평유관。
Objective To investigate the effects of salvianolate and povidone-iodine on postoperative intestinal adhesion in rats. Methods Fifty male SD rats were randomly divided into 5 groups: blank control group, model group, salvianolate group, povidone-iodine group and salvianolate combined with povidone-iodine group(combination group), with 10 rats in each group. A intestinal adhesion modeling was established in rats, and then each group were given the corresponding drugs(intraperitoneal injection of 5% glucose solution, 4mL/kg; salvianolate 10.9mg/kg;0.625% povidone-iodine, 4mL/kg) for 8d. On the 7th day, rats underwent open surgery in abdomen to observe and grade intestinal adhesion by macroscopy. The level of interleukin-1β and transform growth factor-β1 in blood sam-ples and adhesive tissue samples was determined by ELISA. Results Compared with model group, the grade of intestinal adhesion in salvianolate group and combination group was significantly lower (P<0.05);the level of IL-1βin blood samples and tissue samples in salvianolate group, povidone-iodine group and combination group were low-er(blood: 75.75±6.67pg/mL vs 64.82±10.55pg/mL, 70.93±16.06pg/mL, and 62.37±3.32pg/mL; tissue: 220.78±18.31pg/mL vs 178.90±17.28pg/mL, 190.76±34.16pg/mL, 168.40±25.14pg/mL; P<0.05 or P<0.01); the level of TGF-β1 in blood samples and tissue samples in salvianolate group, povidone-iodine group and combination group were lower (blood: 54.93±4.75pg/mL vs 36.77±5.25pg/mL, 42.67±3.40pg/mL, and 21.90±1.15pg/mL; tissue: 759.87±178.42pg/mL vs 639.62 ±118.31pg/mL, 645.32 ±56.95pg/mL, 609.87 ±64.88pg/mL; P<0.05 or P<0.01). Conclusion Both of sal-vianolate and povidone-iodine and their combination can effectively prevent intestinal adhesion after abdominal op-eration in rats, which may be related to reduction of IL-1β and TGF-β1 in peripheral blood and tissues.
