CAJ | 학술논문

目的：探讨香烟提取物（CSE）是否对非洲绿猴肾成纤维细胞（COS-7）表面血栓调节蛋白（TM）荧光蛋白表达的影响。方法：成功构建TM-绿色荧光蛋白（GFP）质粒后，使其转染至COS-7细胞，用制备好的5％ CSE孵育（5％ CSE组）；将无血清最低必需培养基（MEM ，单纯培养基）加入一定体积的PBS进行同步培养作为对照组；采用流式细胞仪计数方法检测不同时间点COS-7表面TM荧光蛋白表达量的变化。结果：与对照组比较，5％CSE在1h、6h对COS-7表面TM荧光蛋白表达没有明显变化[1h ：（134.99±18.41）比（146.61±12.06），6h （116.89±27.28）比（123.89±39.24）， P均＞0.05]。结论：5％香烟提取物对非洲绿猴肾成纤维细胞表面血栓调节蛋白荧光表达没有影响。
목적：탐토향연제취물（CSE）시부대비주록후신성섬유세포（COS-7）표면혈전조절단백（TM）형광단백표체적영향。방법：성공구건TM-록색형광단백（GFP）질립후，사기전염지COS-7세포，용제비호적5％ CSE부육（5％ CSE조）；장무혈청최저필수배양기（MEM ，단순배양기）가입일정체적적PBS진행동보배양작위대조조；채용류식세포의계수방법검측불동시간점COS-7표면TM형광단백표체량적변화。결과：여대조조비교，5％CSE재1h、6h대COS-7표면TM형광단백표체몰유명현변화[1h ：（134.99±18.41）비（146.61±12.06），6h （116.89±27.28）비（123.89±39.24）， P균＞0.05]。결론：5％향연제취물대비주록후신성섬유세포표면혈전조절단백형광표체몰유영향。
Objective:To explore whether cigarette smoking extract (CSE) has influence on fluorescence protein ex-pression of thrombomodulin (TM) on surface of African green monkey kidney fibroblast cells (COS-7) or not . Methods:When TM-green fluorescent protein (GFP) plasmid was successfully constructed ,COS-7 cells were trans-fected by it ,then incubated by prepared 5% CSE (5% CSE group) ,PBS of certain volume was added to serum-free minimal essential medium (MEM ,simple medium) ,which was cultured at the same time and treated as control group .Flow cytometry counting method was used to detect change of TM-GFP expression amount on COS-7 surface at different time point .Results:Compare with control group ,there were no significant difference in the expression of TM-GFP on COS-7 surface at 1h and 6h in 5% CSE group [1h :(134.99 ± 18.41) vs .(146.61 ± 12.06) ,6h :(116.89 ± 27.28) vs .(123.89 ± 39.24) ,P>0.05 both] .Conclusion:The 5% cigarette smoking extract has no influ-ence on fluorescence expression of thrombomodulin on surface of African green monkey kidney fibroblast cells .