中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
6期
1230-1232
,共3页
晋学飞%吴冰%谭九峰%孙利波
晉學飛%吳冰%譚九峰%孫利波
진학비%오빙%담구봉%손리파
膀胱癌%生存素%Livin%zeste同源物-2
膀胱癌%生存素%Livin%zeste同源物-2
방광암%생존소%Livin%zeste동원물-2
Bladder cancer%Survivin%Livin%Enhancer of zeste homolog-2
目的 探讨膀胱癌组织中凋亡抑制基因生存素(Survivin)、Livin和zeste同源物-2(EZH-2)基因的表达及其相关性.方法 随机选取经病理检查明确诊断为膀胱癌的患者83例作为观察组,另选膀胱良性病变患者50例作为对照组,取观察组患者的膀胱癌组织和对照组患者正常的膀胱黏膜作为检测标本,利用免疫组织化学方法对标本中凋亡抑制蛋白Survivin、Livin和EZH-2蛋白染色,观察凋亡抑制基因Survivin、Livin和EZH-2基因的表达,并分析与临床资料的关系.对所有观察组患者进行为期5年的门诊随访.结果 膀胱癌组织中,Survivin、Livin和EZH-2的阳性表达率分别为74.70％、69.88％和83.13％,3种蛋白的阳性表达率均显著高于正常膀胱组织(P＜0.05);Survivin和Livin的阳性表达与性别、TNM分期、病理分级和肿瘤数目无明显相关(P＞0.05),而与组织来源和肿瘤复发明显相关(P＜0.05);EZH-2的阳性表达与性别、肿瘤数目无明显相关(P＞0.05),而与组织来源、TNM分期、病理分级和肿瘤复发明显相关(P＜0.05);Survivin和Livin的表达呈正相关(r=0.744,P＜0.01),Survivin和EZH-2的表达呈正相关(r=0.717,P＜0.05),Livin和EZH-2的表达呈正相关(r=0.683,P＜0.05).结论 Survivin、Livin和EZH-2在膀胱癌组织中的表达显著高于正常膀胱组织,且与肿瘤的发生发展有关,其阳性率的检测有助于判断其预后.
目的 探討膀胱癌組織中凋亡抑製基因生存素(Survivin)、Livin和zeste同源物-2(EZH-2)基因的錶達及其相關性.方法 隨機選取經病理檢查明確診斷為膀胱癌的患者83例作為觀察組,另選膀胱良性病變患者50例作為對照組,取觀察組患者的膀胱癌組織和對照組患者正常的膀胱黏膜作為檢測標本,利用免疫組織化學方法對標本中凋亡抑製蛋白Survivin、Livin和EZH-2蛋白染色,觀察凋亡抑製基因Survivin、Livin和EZH-2基因的錶達,併分析與臨床資料的關繫.對所有觀察組患者進行為期5年的門診隨訪.結果 膀胱癌組織中,Survivin、Livin和EZH-2的暘性錶達率分彆為74.70％、69.88％和83.13％,3種蛋白的暘性錶達率均顯著高于正常膀胱組織(P＜0.05);Survivin和Livin的暘性錶達與性彆、TNM分期、病理分級和腫瘤數目無明顯相關(P＞0.05),而與組織來源和腫瘤複髮明顯相關(P＜0.05);EZH-2的暘性錶達與性彆、腫瘤數目無明顯相關(P＞0.05),而與組織來源、TNM分期、病理分級和腫瘤複髮明顯相關(P＜0.05);Survivin和Livin的錶達呈正相關(r=0.744,P＜0.01),Survivin和EZH-2的錶達呈正相關(r=0.717,P＜0.05),Livin和EZH-2的錶達呈正相關(r=0.683,P＜0.05).結論 Survivin、Livin和EZH-2在膀胱癌組織中的錶達顯著高于正常膀胱組織,且與腫瘤的髮生髮展有關,其暘性率的檢測有助于判斷其預後.
목적 탐토방광암조직중조망억제기인생존소(Survivin)、Livin화zeste동원물-2(EZH-2)기인적표체급기상관성.방법 수궤선취경병리검사명학진단위방광암적환자83례작위관찰조,령선방광량성병변환자50례작위대조조,취관찰조환자적방광암조직화대조조환자정상적방광점막작위검측표본,이용면역조직화학방법대표본중조망억제단백Survivin、Livin화EZH-2단백염색,관찰조망억제기인Survivin、Livin화EZH-2기인적표체,병분석여림상자료적관계.대소유관찰조환자진행위기5년적문진수방.결과 방광암조직중,Survivin、Livin화EZH-2적양성표체솔분별위74.70％、69.88％화83.13％,3충단백적양성표체솔균현저고우정상방광조직(P＜0.05);Survivin화Livin적양성표체여성별、TNM분기、병리분급화종류수목무명현상관(P＞0.05),이여조직래원화종류복발명현상관(P＜0.05);EZH-2적양성표체여성별、종류수목무명현상관(P＞0.05),이여조직래원、TNM분기、병리분급화종류복발명현상관(P＜0.05);Survivin화Livin적표체정정상관(r=0.744,P＜0.01),Survivin화EZH-2적표체정정상관(r=0.717,P＜0.05),Livin화EZH-2적표체정정상관(r=0.683,P＜0.05).결론 Survivin、Livin화EZH-2재방광암조직중적표체현저고우정상방광조직,차여종류적발생발전유관,기양성솔적검측유조우판단기예후.
Objective To investigate the expression of survivin,livin and enhancer of zeste homolog-2 (EZH-2) in bladder cancer tissues and their correlation with bladder cancer.Methods Eighty-three patients pathological diagnosed as bladder cancer were randomly selected as the observation group.Fifty patients with bladder benign lesions served as the control group.The bladder cancer tissues from observation group and the normal bladder mucosa sanples from control group were obtained detection of survivin,livin and EZH-2.The relationship between the expression of survivin,livin and EZH-2 with the clinical data was analyzed.All patients were subjected to 5-year outpatient follow-up.Results In the bladder cancer tissues,the positive expression rate of survivin,livin and EZH-2 was 74.70％,69.88％ and 83.13％ respectively,significantly higher than in normal bladder tissues correspondingly (P ＜ 0.05).The positive expression of survivin and livin was not related with sex,TNM stage,pathological grading and the number of tumor (P ＞ 0.05),but related to tissue origin and tumor recurrence (P ＜ 0.05).The positive expression of EZH-2 had no correlation with the number of tumor and gender (P ＞ 0.05),but related with tissue origin,TNM staging,pathological grading and recurrence of the tumor (P ＜ 0.05).There was a positive correlation between survivin and livin (r =0.744,P ＜ 0.01),or between survivin and EZH-2 (r =0.717,P ＜ 0.05),or between livin and EZH-2 (r =0.683,P ＜ 0.05).Conclusion The expression of survivin,livin and EZH-2 in bladder cancer tissues was significantly higher than that in normal bladder tissue,and related to the occurrence and development of tumor.The detection of the positive expression rate of survivin,livin and EZH-2 can evaluate the prognosis.
